Abstract
A mutant considered to be defective in the conversion of n-6 to n-3 fatty acids (ω3-desaturation) was derived from a Δ5-desaturation-defective mutant (Mut44) of Mortierella alpina 1S-4, after treating its spores with N-methyl-N′-nitro-N-nitrosoguanidine. This mutant cannot produce 8(Z),11(Z),14(Z),17(Z)-eicosatetraenoic acid or any other n-3 fatty acids, of which about 10% was found in its parental strain upon cultivation at 12°C. The mutant's growth rate was comparable to that of the parental strain when grown at 28°C, but it became much slower when the mutant grew at 12°C, at which the lag phase for Mut44 was about 2 d but 5 d for the mutant.
Similar content being viewed by others
Abbreviations
- 18:3ω3:
-
9(Z),12(Z),15(Z)-octadecatrienoic acid
- 18:4ω3:
-
6(Z),9(Z),12(Z),15(Z)-octadecatetraenoic acid
- 20:4ω3:
-
8(Z),11(Z),14(Z),17(Z)-eicosatetraenoic acid
- AA:
-
arachidonic acid
- DHGA:
-
dihomo-γ-linolenic acid
- EPA:
-
5(Z),8(Z),11(Z),14(Z),17(Z)-eicosapentaenoic acid
- GLC:
-
gas-liquid chromatography
- MNNG:
-
N-methyl-N′-nitro-N-nitrosoguanidine
- PC:
-
phosphatidylcholine
References
ChristieWW, NobleRC, MooreJH (1970) Determination of lipid classes by a gas-chromatography procedure. Analyst 95: 940 to 944
FolchJ, LeesM, Sloane-StanleyGH (1957) A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509
GellermanJL, SchlenkH (1979) Methyl-directed desaturation of arachidonic acid to eicosapentaenoic acid in the fungus, Saprolegnia parasitica. Biochim Biophys Acta 573: 23–30
JareonkitmongkolS, KawashimaH, ShirasakaN, ShimizuS, YamadaH (1992a) Production of dihomo-γ-linolenic acid by a Δ5-desaturase-defective mutant of Mortierella alpina 1S-4. Appl Environ Microbiol 58: 2196–2200
JareonkitmongkolS, ShimizuS, YamadaH (1992b) Fatty acid desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. J Gen Microbiol 138: 997–1002
JareonkitmongkolS, ShimizuS, YamadaH (1993) Occurrence of two nonmethylene-interrupted Δ5-polyunsaturated fatty acids in a Δ6-desaturase-defective mutant of the fungus Mortierella alpina 1S-4. Biochim Biophys Acta 1167: 137–141
ShimizuS, ShinmenY, KawashimaH, AkimotoK, YamadaH (1988) Fungal mycelia as a novel source of eicosapentaenoic acid: activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature. Biochem Biophys Res Commun 150: 335–341
ShimizuS, AkimotoK, KawashimaH, ShinmenY, YamadaH (1989) Production of dihomo-γ-linolenic acid by Mortierella alpina 1S-4. J Am Oil Chem Soc 66: 237–241
ShimizuS, JareonkitmongkolS, KawashimaH, AkimotoK, YamadaH (1991) Production of a novel ω1-eicosapentaenoic acid by Mortierella alpina 1S-4 grown on 1-hexadecene. Arch Microbiol 156: 163–166
ShinmenY, ShimizuS, AkimotoK, KawashimaH, YamadaH (1989) Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production. Appl Microbiol Biotech 31: 11–16
YamadaH, ShimizuS, ShinmenY (1987) Production of arachidonic acid by Mortierella elongata 1S-5. Agric Biol Chem 51: 785–790
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jareonkitmongkol, S., Sakuradani, E. & Shimizu, S. Isolation and characterization of an ω3-desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. Arch. Microbiol. 161, 316–319 (1994). https://doi.org/10.1007/BF00303586
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00303586