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Demonstration of the neural crest origin of type I (APUD) cells in the avian carotid body, using a cytochemical marker system

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Summary

The biogenic amines present in the carotid body Type 1 cells of two avian species (Japanese quail and chicken) were identified, by microspectrofluorometry of formaldehyde-induced fluorescence, as dopamine and 5-hydroxytryptamine respectively. These and other cytochemical properties establish the cells as members of the APUD series.

Grafts of the neural rhombencephalic primordium from 6 to 10-somite quail embryos were implanted in the appropriate region of chick embryos of the same age. After up to 11 days incubation the carotid bodies of the host were freeze-dried and treated with hot formaldehyde vapour. The carotid body Type 1 cells in the chick host were identified, by the presence of dopamine and the absence of 5-HT, as cells from the quail neural crest.

The dopamine phenotype in cells of quail origin thus provides a cytochemical marker which may be used for other allograft experiments. The present work confirms earlier findings, using a biological (nuclear chromatin) marker, which showed the avian carotid body to be of neural crest origin.

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Authors and Affiliations

  1. Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK

    A. G. E. Pearse, J. M. Polak, F. W. D. Rost, J. Fontaine, C. Le Lièvre & N. Le Douarin

  2. Laboratoire d'Embryologie de la Faculté des Sciences de Nantes, Cedex Nantes, France

    A. G. E. Pearse, J. M. Polak, F. W. D. Rost, J. Fontaine, C. Le Lièvre & N. Le Douarin

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  1. A. G. E. Pearse
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  2. J. M. Polak
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  4. J. Fontaine
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  6. N. Le Douarin
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Pearse, A.G.E., Polak, J.M., Rost, F.W.D. et al. Demonstration of the neural crest origin of type I (APUD) cells in the avian carotid body, using a cytochemical marker system. Histochemie 34, 191–203 (1973). https://doi.org/10.1007/BF00303435

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