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Direct versus cultured preparation of bone marrow cells from 22 patients with acute myeloid leukemia

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Summary

The 24-h culture of bone marrow from patients with acute myeloblastic leukemia (AML) and acute promyelocytic leukemia (APL) gave more analyzable metaphase cells and improved chromosome morphology compared with direct preparations. Culture increased the proportion of cytogenetically abnormal cells, and in six bone marrows where the direct preparation failed, a result was obtained from the cultured preparation. The culture of bone marrow from patients with APL led to the detection of clones carrying the t(15;17) that were not found in direct preparations. Such sequestered clones were not found in AML and acute myelomonocytic leukemia (AMMoL). Cultured preparations were no better than direct preparations from AMMoL.

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References

  • Berger R, Bernheim A, Flandrin G (1980) Absence d'anomalic chromosomique et leucemie aigue: relations avec les cellules medullaires normales. CR Acad Sci [D] (Paris) 290:1557–1559

    Google Scholar 

  • Ford CE, Jacobs PA, Lajtha LG (1985) Human somatic chromosomes. Nature 181:1565–1568

    Google Scholar 

  • Knuutila S, Vuopio P, Borgstrom GH, de la Chapelle A (1980) Higher frequency of 5q-clone in bone marrow mitoses after culture than by a direct method. Scand J Haematol 25:358–362

    Google Scholar 

  • Knuutila S, Vuopio P, Elonen E, Siimes M, Kovanen R, Borgstrom GH, de la Chapelle A (1981) Culture of bone marrow reveals more cells with chromosomal abnormalities than the direct method in patients with hematologic disorders. Blood 58:369–375

    Google Scholar 

  • Nowell PC, Hungerford DA (1960) Chromosome studies in normal and leukemic human leukocytes. J Natl Cancer Inst 25:85–109

    Google Scholar 

  • Sakurai M, Sandberg AA (1973) Prognosis of acture myeloblastic leukemia: chromosomal correlation. Blood 41:93–104

    Google Scholar 

  • Sakurai M, Sandberg AA (1974) Chromosomes and causation of human cancer and leukemia. IX. Prognostic and therapeutic value of chromosomal findings in acute myeloblastic leukemia. Cancer 33:1548–1557

    Google Scholar 

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Authors and Affiliations

  1. Cancer Society of New Zealand, Cytogenetics Unit, Christchurch Hospital, Christchurch, New Zealand

    Peter H. Fitzgerald, Christine M. Morris & Lynette M. Giles

Authors
  1. Peter H. Fitzgerald
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  2. Christine M. Morris
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  3. Lynette M. Giles
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Fitzgerald, P.H., Morris, C.M. & Giles, L.M. Direct versus cultured preparation of bone marrow cells from 22 patients with acute myeloid leukemia. Hum Genet 60, 281–283 (1982). https://doi.org/10.1007/BF00303020

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  • DOI: https://doi.org/10.1007/BF00303020

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