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Mitotic index and size of symbiotic algae in Caribbean Reef corals

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Abstract

Size and frequency of division were determined for zooxanthellae from nine scleractinian coral species collected in February, 1983 at Discovery Bay, Jamaica, from four depths over a 51 m bathymetric range. Mean diameters of zooxanthellae ranged from 6.4 to 12.6 μm. Small zooxanthellae were found in Madracis mirabilis, Eusmilia fastigiata and Dendrogyra cylindrus whereas larger cells were seen in Agaricia agaricites, Porites astreoides, Montastrea cavernosa and Acropora cervicornis. Zooxanthellae division was not phased over a diel cycle. The percentage of zooxanthellae in a paired stage of cytokinesis (mitotic index or MI) was highly variable and ranged from 1.1% to 14.1%. Values measured in E. fastigiata and D. cylindrus were greater than in the other corals. MI was higher in branch tips of A. cervicornis than in branch bases. Daily average MI was negatively correlated with mean cell diameter and for a majority of the coral species increased with habitat depth. MI values were used to estimate specific growth rates and generation times for zooxanthellae in vivo for comparison with other dinoflagellates.

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References

  • Apstein C (1911) Biologische Studie über Ceratium tripos var. subsalsa. Ostf Wiss Meeresunters NF 12:137–162

    Google Scholar 

  • Bak RPM (1976) The growth of coral colonies and the importance of crustose coralline algae and burrowing sponges in relation with carbonate accumulation. Neth J Sea Res 10:285–337

    Google Scholar 

  • Blank RJ, Trench RK (1985) Speciation and symbiotic dinoflagellates. Science 229:656–658

    Google Scholar 

  • Blasco D, Packard TT, Garfield PC (1982) Size dependence of growth rate, respiratory transport system activity, and chemical composition in marine diatoms in the laboratory. J Phycol 18:58–63

    Google Scholar 

  • Chang SS, Prezelin BB, Trench RK (1983) Mechanisms of photoadaptation in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum. Mar Biol 76:219–229

    Google Scholar 

  • Chisholm SW (1981) Temporal patterns of cell division in unicellular algae. In: Platt T (ed) Physiological bases of phytoplankton ecology. Can Bull Fish Aquat Sci 210:150–181

  • Davies PS (1984) The role of zooxanthellae in the nutritional energy requirements of Pocillopora eydouxi. Coral Reefs 2:181–186

    Google Scholar 

  • Deane EM, O'Brien RW (1978) Isolation and axenic culture of Gymnodinium microadriaticum from Tridacna maxima. Br Phycol J 13:189–195

    Google Scholar 

  • Domotor SL, D'Elia CF (1984) Nutrient uptake kinetics and growth of zooxanthellae maintained in laboratory culture. Mar Biol 80:93–101

    Google Scholar 

  • Domotor SL, D'Elia CF (1986) Cell-size distributions of zooxanthellae in culture and symbiosis. Biol Bull 170:519–526

    Google Scholar 

  • Doyle WL, Doyle MM (1940) The structure of zooxanthellae. Pap Tortugas Lab 32:127–142

    Google Scholar 

  • Drew EA (1972) The biology and physiology of algal-invertebrate symbiosis. I. The density of algal cells in a number of hermatypic hard corals and alcyonarians from various depths. J Exp Mar Biol Ecol 9:71–75

    Google Scholar 

  • Dustan P (1979) Distribution of zooxanthellae and photosynthetic chloroplast pigments of the reef-buiding coral Montastrea annularis Ellis and Solander in relation to depth on a West Indian coral reef. Bull Mar Sci 29:79–95

    Google Scholar 

  • Fitt WK, Trench RK (1983) The relation of diel patterns of cell division to diel patterns of motility in the symbiotic dinoflagellate Symbiodinium microadriaticum Freudenthal in culture. New Phytol 94:421–432

    Google Scholar 

  • Gladfelter EH (1983) Spatial and temporal patterns of mitosis in the cells of the axial polyp of the reef coral Acropora cervicornis. Biol Bull 165:811–815

    Google Scholar 

  • Glynn PW (1983) Extensive “bleaching” and death of reef corals on the Pacific coast of Panama. Environ Conserv 10:149–154

    Google Scholar 

  • Glynn PW (1984) Widespread coral mortality and the 1982/1983 El Nino warming event. Environ Conserv 11:133–146

    Google Scholar 

  • Gran HH (1912) Pelagic plant life. In: Murray J, Hjort J (eds) The depths of the ocean. MacMillan, London, pp 307–386

    Google Scholar 

  • Heller MD (1977) The phased division of the freshwater dinoflagellate Ceratium hirundinella and its use as a method of assessing growth in natural populations. Freshwater Biol 7:527–533

    Google Scholar 

  • Hoegh-Gulberg O, McCloskey LR, Muscatine L (1987) Expulsion of zooxanthellae by symbiotic cnidarians from the Red Sea. Coral Reefs 5:201–204

    Google Scholar 

  • Huston M (1985) Variation in coral growth rates with depth at Discovery Bay, Jamaica. Coral Reefs 4:19–27

    Google Scholar 

  • Johannes RE, Wiebe WJ (1970) A method for determination of coral tissue biomass and composition. Limnol Oceanogr 15:822–824

    Google Scholar 

  • Lewis DH, Smith DC (1971) The autotrophic nutrition of symbiotic marine coelenterates with special references to hermatypic corals. I. Movement of photosynthetic products between the symbionts. Proc R Soc London 178:111–129

    Google Scholar 

  • Lewis JB (1977) Processes of organic production on coral reefs. Biol Rev 52:305–347

    Google Scholar 

  • Lewis JB (1981) Estimates of secondary production of reef corals. Proc 4th Int Coral Reef Symp 2:369–374

    Google Scholar 

  • Loeblich AR III, Sherley JL (1979) Observations on the theca of the motile phase of free-living and symbiotic isolates of Zooxanthella microadriatica (Freudenthal) Comb. Nov. J Mar Biol Assoc UK 59:195–205

    Google Scholar 

  • McCloskey LR, Muscatine L (1984) Production and respiration in the Red Sea coral Stylophora pistillata as a function of depth. Proc R Soc London Ser B 222:215–230

    Google Scholar 

  • McDuff RE, Chisholm SW (1982) The calculation of in situ growth rates of phytoplankton populations from fractions of cells undergoing mitosis: a clarification. Limnol Oceanogr 27:783–788

    Google Scholar 

  • Meeson BW, Sweeney BZ (1982) Adaptation of Ceratium furca and Gonyaulax polyedra (Dinophyceae) to different temperatures and irradiances: growth rates and cell volumes. J Phycol 18:241–245

    Google Scholar 

  • Muscatine L (1980) Uptake, retention and release of dissolved inorganic nutrients by marine alga-invertebrate associations. In: Cook CB, Pappas PW, Rudolph ED (eds) Cellular interactions in symbiosis and parasitism. Ohio State University Press, Columbus, pp 229–244

    Google Scholar 

  • Muscatine L, Falkowski PG, Dubinsky Z (1983) Carbon budgets in symbiotic associations. In: Schenk HEA, Schwemmler W (eds) Endocytobiology. II. Intracellular space as oligogenetic ecosystem. de Gruyter, Berlin, pp 649–658

    Google Scholar 

  • Muscatine L, Falkowski PG, Porter JW, Dubinsky Z (1984) Fate of photosynthetic fixed carbon in light-and shade-adapted colonies of the symbiotic coral Stylophora pistillata. Proc R Soc London Ser B 222:181–202

    Google Scholar 

  • Muscatine L, McCloskey LR, Loya Y (1985) A comparison of the growth rates of zooxanthellae and animal tissue in the Red Sea coral Stylophora pistillata. Proc 5th Int Coral Reef Symp 6:119–123

    Google Scholar 

  • Muscatine L, Wilkerson FP, McCloskey LR (1986) Regulation of population density of symbiotic algae in a tropical marine jellyfish (Mastigias sp.) Mar Ecol Prog Ser 32:279–290

    Google Scholar 

  • Parker GT (1984) Photophysiology of the symbiotic sea anemone Aiptasia pulchella. PhD thesis, University of California, Los Angeles

  • Patton JS, Burris JE (1983) Lipid synthesis and extrusion by freshly isolated zooxanthellae (symbiotic algae). Mar Biol 75:131–136

    Google Scholar 

  • Porter JW (1976) Autotrophy, heterotrophy and resource partitioning in Caribbean reef-building corals. Am Nat 110:731–742

    Google Scholar 

  • Porter JW, Muscatine L, Dubinsky Z, Falkowski PG (1984) Primary production and photoadaptation in light-and shade-adapted colonies of the symbiotic coral, Stylophora pistillata. Proc R Soc London 222:161–180

    Google Scholar 

  • Schoenberg DA, Trench RK (1980a) Genetic variation in Symbiodinium (=Gymnodinium) microadriaticum Freudenthal, and specificity in its symbiosis with marine invertebrates. I. Isoenzyme and soluble protein patterns of axenic cultures of Symbiodinium microadriaticum. Proc R Soc London 207:405–427

    Google Scholar 

  • Schoenberg DA, Trench RK (1980b) Genetic variation in Symbiodinium (=Gymnodinium) microadriaticum Freudenthal, and specificity in its symbiosis with marine invertebrates. II. Morphological variation in Symbiodinium microadriaticum. Proc R Soc London 207:429–444

    Google Scholar 

  • Smith GJ (1984) Ontogenetic variation in the symbiotic associations between zooxanthellae (Symbiodinium microadriaticum Freudenthal) and sea anemore (Anthozoa: Actinaria) hosts. PhD thesis, University of Georgia, Athens

    Google Scholar 

  • Smith GJ (1986) Ontogenetic influences on carbon flux in Aulactinia stelloides polyps (Anthozoa: Actinaria) and their endosymbiotic algae. Mar Biol 92:361–371

    Google Scholar 

  • Steele RD (1976) Light intensity as a factor in the regulation of the density of symbiotic zooxanthellac in Aiptasia tagetes. J Zool London 179:387–405

    Google Scholar 

  • Steen RG, Muscatine L (1984) Daily budgets of photosynthetically fixed carbon in symbiotic zoanthids. Biol Bull 167:477–487

    Google Scholar 

  • Swift E, Durbin EG (1972) The phased division and cytological characteristics of Pyrocystis sp. can be used to estimate doubling times of their populations in the sea. Deep-Sea Res 19:189–198

    Google Scholar 

  • Swift E, Stuart M, Meunier V (1976) The in situ growth rates of some deep-living oceanic dinoflagellates: Pyrocystis fusiformis and Pyrocystis noctiluca. Limnol Oceanogr 21:418–426

    Google Scholar 

  • Trench RK (1971) The physiology and biochemistry of zooxanthellae symbiotic with marine coelenterates. II. Liberation of fixed 14C by zooxanthellae in vitro. Proc R Soc London Ser B 177:237–250

    Google Scholar 

  • Weiler CS (1980) Population structure and in situ division rates of Ceratium in oligotrophic waters of the North Pacific central gyre. Limnol Oceanogr 25:610–619

    Google Scholar 

  • Weiler CS, Chisholm SW (1976) Phased cell division in natural populations of marine dinoflagellates from shipboard cultures. J Exp Mar Biol Ecol 25:239–247

    Google Scholar 

  • Wilkerson FP, Trench RK (1986) Uptake of dissolved inorganic nitrogen by the symbiotic clam Tridacna gigas and the coral Acropora sp. Mar Biol 93:237–246

    Google Scholar 

  • Wilkerson FP, Parker GT, Muscatine L (1983) Temporal patterns of cell division in natural populations of endosymbiotic algae. Limnol Oceanogr 28:1009–1014

    Google Scholar 

  • Yonge CM (1973) The nature of reef-building (hermatypic) corals. Bull Mar Sci 23:1–15

    Google Scholar 

  • Yonge CM, Nicholls AG (1931) Studies on the physiology of corals. IV. The structure, distribution and physiology of zooxanthellae. Sci Rep Great Barrier Reef Exped 1:135–176

    Google Scholar 

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Author notes

  1. F. P. Wilkerson

    Present address: Allan Hancock Foundation, University of Southern California, University Park, 90089-0371, Los Angeles, California, USA

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  1. Department of Biology, University of California, 90024, Los Angeles, California, USA

    F. P. Wilkerson, D. Kobayashi & L. Muscatine

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  1. F. P. Wilkerson
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  2. D. Kobayashi
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  3. L. Muscatine
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Wilkerson, F.P., Kobayashi, D. & Muscatine, L. Mitotic index and size of symbiotic algae in Caribbean Reef corals. Coral Reefs 7, 29–36 (1988). https://doi.org/10.1007/BF00301979

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