Summary
The trunk musculature of adult zebrafishes contains three major fiber types: adult red, intermediate, and white; and two minor populations: red muscle rim and scattered intermediate fibers.
In this paper, the post hatching development of these muscle fiber types was studied by means of immunohistochemistry, using anti-myosin sera. Just hatched larvae contain two muscle fiber populations: embryonic red and white which give rise to the red muscle rim and the intermediate fibers respectively. Adult red fibers arise post hatching as a new separate population with distinct myosin properties.
The differentiation of these fiber types occurs within the first four weeks after fertilization, when the adult pattern of peripheral axon bundles has become established.
Differences in the muscle fiber type composition between the midbody and the tail myotomes become apparent in two month old fries. The number of scattered intermediate fibers increases from rostral to caudal, the opposite holds for the red muscle rim fibers. The red and intermediate area is triangular in the midbody; in the tail part it is stretched out along the lateral surface of the myotomes. These changes are considered as adaptations to improve the efficiency of the swiming performance.
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References
Boddeke R, Slijper EJ, Stelt A van der (1959) Histological characteristics of the body musculature of fishes in connection with their mode of life. Proc K Ned Akad Wet Ser C 62:576–588
Bone Q, Kiceniuk J, Jones DR (1979) On the role of the different fibre types in fish myotomes at intermediate swimming speeds. Fish Bull 76:691–699
Carpene E, Vegetti A (1981) Increase of muscle fibres in the lateralis muscle (white portion) of mugulidae (Pisces, Teleostei). Experientia 37:191–193
Flood PR, Kryvi H, Gulyaev D (1981) The origin and early differentiation of red and white muscle fibres in the sturgeon (Acipenser stellatus) (In preparation)
Gauthier GF (1980) Distribution of myosin isoenzymes in adult and developing muscle fibers. In: Pette D (ed) Plasticity of muscle. De Gruyter, Berlin, pp 83–96
Guth L, Samaha FJ (1972) Erroneous interpretations which may result from application of the “myofibrillar ATP-ase histochemical procedure” to developing muscle. Exp Neurol 34:465–475
Hisaoka KK, Battle HI (1958) The normal developmental stages of the zebrafish Brachydanio rerio (Hamilton Buchanan). J Morphol 102:311–327
Johnston IA, Ward PS, Goldspink G (1975) Studies on the swimming musculature of the rainbowtrout. I. Fibre types. J Fish Biol 7:451–458
Johnston IA, Davison W, Goldspink G (1977) Energy metabolism of carp swimming muscle. J Comp Physiol 114:203–216
Kimmel CB, Patterson J, Kimmel RO (1974) The development and behavioural characteristics of the startle response in the zebrafish. Dev Psychobiol 7:47–60
Liem RKH, Yen SH, Salomon GD, Shelanski ML (1978) Intermediate filaments in nervous tissue. J Cell Biol 79:637–645
Mosse PRL, Hudson RCL (1977) The functional roles of different muscle fibre types identified in the myotomes of marine teleosts: a behavioural anatomical and histochemical study. J Fish Biol 11:417–430
Müller W (1974) Temporal progress of muscle adaptation to endurance training in hindlimb muscles of young rats. Cell Tissue Res 156:61–87
Nag AC, Nursall JR (1972) Histogenesis of white and red muscle fibres of trunk muscles of a fish Salmo gairdneri. Cytobios 6:227–246
Perry SV, Dooth GK, (1980) Biochemical aspects of muscle development and differentiation. In: Goldspink DF (ed). Development and specialization of skeletal muscle. Cambridge Un Press, Cambridge
Pool CW (1980) An immune-and enzyme-histochemical determination of striated muscle fibre characteristics. Thesis Univ Amsterdam, Rodopi, Amsterdam pp 142–167
Proctor C, Mosse PRL, Hudson RCL (1980) A histochemical and ultrastructural study of the development of the propulsive musculature of the brown trout, Salmo trutta L., in relation to its swimming behaviour. J Fish Biol 16:309–329
Raamsdonk W van, Stelt A van der, Diegenbach PC, Berg W van de, Bruyn H de, Dijk J van, Mijzen P (1974) Differentiation of the musculature of the teleost Brachydanio rerio. I. Myotome shape and movements in the embryo. Z Anat Entwickl Ges 145:321–342
Raamsdonk W van, Pool CW, Heyting C (1977) Detection of antigens and antibodies by an immuno-peroxidase method applied on thin longitudinal sections of SDS-polyacrylamide gels. J Immunol Meth 17:337–348
Raamsdonk W van, Pool CW, Kronnie G te (1978) Differentiation of muscle fiber types in the teleost Brachydanio retio. Anat Embryol 153:137–155
Raamsdonk W van, teKronnie G, Pool CW, Laarse W van de (1980) An immune histochemical and enzymic characterization of the muscle fibers in myotomal muscle of the teleost Brachydanio rerio, Hamilton Buchanan. Acta Histochem 67:200–216
Rubinstein NA, Kelly AM (1981) Development of muscle fiber specialization in the rat hindlimb. J Cell Biol 90:128–144
Salmons S, Henriksson J (1981) The adaptive response of skeletal muscle to increased use. Muscle and Nerve 4:94–105
Sternberger LA (1979) Immunocytochemistry. John Wiley and Sons, New York
Waterman RE (1969) Development of the lateral musculature in the teleost Brachydanio rerio: a fine-structural study. Am J Anat 125:457–494
Weatherley AH, Gill HS, Rogers SC (1980) The relationship between mosaic muscle fibres and size in rainbow trout (Salmo gairdneri). J Fish Biol 17:603–611
Willemse JJ, Berg PG van de (1978) Growth of striated muscle fibre in the m-lateralis of the european eel Anguilla anguilla (L.) (Pisces, Teleostei). J Anat 125:447–460
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van Raamsdonk, W., van't Veer, L., Veeken, K. et al. Differentiation of muscle fiber types in the teleost Brachydanio rerio, the zebrafish. Anat Embryol 164, 51–62 (1982). https://doi.org/10.1007/BF00301878
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DOI: https://doi.org/10.1007/BF00301878