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Induction and activation of procollagenase in rabbit synovial fibroblasts after treatment with active oxygen released by xanthine/xanthine oxidase

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Abstract

Treatment of rabbit synovial fibroblasts with active oxygen (AO) released by xanthine/xanthine oxidase resulted in an induction of procollagenase in these cells in concentrations ranging from 12.5 μg/ml xanthine plus 0.0025 U/ml xanthine oxidase to 50 μg/ml xanthine plus 0.01 U/ml xanthine oxidase. Preceding this there was an accumulation of poly(ADP-ribose) for the same concentration range of xanthine/xanthine oxidase. Furthermore, it was found that AO caused activation of the latent procollagenase to the active enzyme in concentrations ranging from 0.1 μg/ml xanthine plus 0.00002 U/ml xanthine oxidase to 1 μg/ml xanthine plus 0.0002 U/ml xanthine oxidase. It is suggested that poly(ADP-ribosyl)ation participates in the induction of procollagenase by relaxing chromatin. Furthermore, it is proposed that AO activates latent procollagenase under physiological conditions.

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  1. H. Kröger

    Present address: Deutsches Rheumaforschungszentrum Berlin, Nordufer 20, D-13353, Berlin, Germany

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  1. Robert Koch-Institut, Nordufer 20, D-13353, Berlin, Germany

    W. Ehrlich, H. Huser & H. Kröger

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Ehrlich, W., Huser, H. & Kröger, H. Induction and activation of procollagenase in rabbit synovial fibroblasts after treatment with active oxygen released by xanthine/xanthine oxidase. Rheumatol Int 15, 131–136 (1995). https://doi.org/10.1007/BF00301769

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  • DOI: https://doi.org/10.1007/BF00301769

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