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Structure-activity relationships on hyperglycemia by representatives of the adipokinetic/hyperglycemic hormone family in Blaberus discoidalis cockroaches

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Summary

Several members of the adipokinetic/hyperglycemic neurohormone family from several different invertebrate species have been prepared by solid-phase peptide synthesis and assayed by a modified in vivo hyperglycemic bioassay in Blaberus discoidalis cockroaches. The hypertrehalosemic hormone (HrTH) is the endogenous hypertrehalosemic factor for B. discoidalis and was the most potent peptide in the assay. The more divergent the sequence of a family member from Blaberus HrTH, the less potent was the bioanalog. Manduca adipokinetic hormone is the most divergent peptide of the family and was totally inactive in the bioassay. Locusta adipokinetic hormone I had reduced maximum activity in the assay, which suggests that Ser5 is an important residue for the transduction of the hyperglycemic response. The direct relation between bioanalog similarity to Blaberus HrTH sequence and potency suggests that the hormone and target cell receptor for HrTH have evolved to maintain an “optimal fit”.

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Abbreviations

AKH :

adipokinetic hormone

HrGH :

hyperglycemic hormone

HrTH :

hypertrehalosemic hormone

RPCH :

red pigmentconcentrating hormone

CAH:

cardioacceleratory hormone. Hormone abbreviations are according to the convention of Gäde and Raina (1989) except that the genus names are not abbreviated

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  1. Laboratories for Invertebrate Neuroendocrine Research, Department of Entomology, Texas Agricultural Experiment Station, Texas A&M University, 77843, College Station, Texas, USA

    Timothy K. Hayes & Larry L. Keeley

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  1. Timothy K. Hayes
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  2. Larry L. Keeley
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Hayes, T.K., Keeley, L.L. Structure-activity relationships on hyperglycemia by representatives of the adipokinetic/hyperglycemic hormone family in Blaberus discoidalis cockroaches. J Comp Physiol B 160, 187–194 (1990). https://doi.org/10.1007/BF00300952

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