Summary
Catecholamines were found histochemically in the visceral yolk sac of the rat from embryonic day (ED) 10, i.e. before the amines become detectable in peripheral or central neurons of the fetus. Formaldehydeinduced fluorescence was confined to the apical part of the yolk sac epithelial cells. The specificity of histofluorescence has been confirmed by borohydride reduction, microspectrofluorimetry revealing an emission peak at 480 nm and administration of reserpine. The catecholamines present were identified by mass fragmentography using N,O-trifluoroacetyl derivatives. At ED 13 both dopamine and norepinephrine were present, while only dopamine was detected at ED 181/2. Maternal circulation or the epithelial cells themselves appear as possible sources of these catecholamines. The occurrence of amines in the yolk sac epithelium may reflect an intracellular role of these compounds, a barrier function of the epithelium or a step in a transport to the fetus where the amines might assume regulatory functions.
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References
Allan, I.J., Newgreen, D.F.: Catecholamine accumulation in neural crest cells and the primary sympathetic chain. The American J. of Anatomy 149, 413–421 (1977)
Asai, T.: Zur Entwicklung und Histophysiologie des Dottersacks der Nager mit Entypie des Keimfeldes. Anatomische Hefte 51, 467–642 (1914)
Beck, F., Lloyd, J.B., Griffiths, A.: A histochemical and biochemical study of some aspects of placental function in the rat using maternal injection of horse radish peroxidase. J. Anat. 101, 461–478 (1967)
Bourne, B.B.: Metabolism of amines in the brain of the chick during embryonic development. Life Sciences 4, 583–591 (1965)
Burden, H.W., Lawrence, I.F.: Presence of biogenic amines in early rat development. Am. J. Anat. 136, 251–257 (1973)
Buznikov, G.A., Kost, A.N., Kucherova, N.F., Mndzhoyan,, A.L., Suvorov, N.N., Berdysheva, L.V.: The role of neurohumors in early embryogenesis. III. Pharmacological analysis of the role of neurohumors in cleavage divisions. J. Embryol. Exp. Morphol. 23, 549–569 (1970)
Buznikov, G.A., Chudakova, I.V., Berdysheva, L.V., Vyazmina, N.M.: The role of neurohumors in early embryogenesis. II. Acetylcholine and catecholamine content in developing embryos of sea urchin. J. Embryol. Exp. Morphol. 20, 119–128 (1968)
Cegrell, L.: The occurrence of biogenic monoamines in the mammalian endocrine pancreas. Acta physiologica Scand. Suppl. 314 (1968)
Chan, S.T.H., Wong, P.Y.D.: Evidence of active sodium transport in the visceral, yolk sac of the rat in vitro. J. Physiol. 279, 385–394 (1978)
Corrodi, H., Hillarp, N.A., Jonsson, G.: Fluorescence methods for the histochemical demonstration of monoamines. 3. Sodium borohydride reduction of the fluorescent compounds as a specificity test. J. Histochem. Cytochem. 12, 582–586 (1964)
Curtius, H.Ch., Wolfensberger, M., Steinmann, B., Redweik, U., Siegfried, J.: Massfragmentography of Dopamine and 6-Hydroxydopamine. Application to the determination of Dopamin in Human brain biopsies from Caudate nucleus. J. of Chromatography 99, 529–540 (1974)
Falck, B., Owman, Ch.: 5-Hydroxytryptamine and related amines in endocrine cells. Advances in Pharmacology 6, 211–231 (1968)
Grosser, O.: Vergleichende Anatomie und Entwicklungsgeschichte der Eihäute und der Placenta. Wien und Leipzig: Wilhelm Braumüller 1909
Ignarro, L.J., Shideman, F.E.: The requirement of sympathetic innervation for the active transport of Norepinephrine by the heart. J. Pharmacol. Exp. Ther. 159, 59–65 (1968a)
Ignarro, L.J., Shideman, F.E.: Norepinephrine and epinephrine in the embryo and embryonic heart of the chick: uptake and subcellular distribution. J. Pharmacol. Exp. Ther., 159, 49–58 (1968b)
Kühnel, W.: Histologische Untersuchungen an den Embryonalhüllen der weißen Maus. Z. Zellforsch. 63, 194–207 (1964)
Lauder, J.M., Bloom, F.E.: Ontogeny of monoamine neurons in the Locus coeruleus, Raphe nuclei and Substantia nigra of the rat. J. Comp. Neurology 155, 469–482 (1974)
Lawrence, I.E., Burden, H.W.: Catecholamines and morphogenesis of the chick neural tube and notochord. Am. J. Anat. 137, 199–208 (1973)
Lichtensteiger, W.: Cyclic variations of catecholamine content in hypothalamic nerve cells during estrous cycle of the rat, with a concomitant study of the substantia nigra. J. Pharmacol. Exp. Ther. 165, 204–215 (1969)
Litwer, G.: Über die Sekretion und Resorption in den Dotterentodermzellen bei graviden weißen Mäusen. Z. Zellforsch. 8, 135–152 (1928)
McCarthy, L.P., Lee, W.C., Shideman, F.E.: Measurement of the inotropic effects of drugs on the innervated and non innervated embryonic chick heart. J. Pharmacol. Exp. Ther. 129, 315–321 (1960)
Mayersbach, H.: Zur Frage des Proteinüberganges von der Mutter zum Foeten. Z. Zellforschg. 48, 479–504 (1958)
Morgan, D.C., Sandler, M., Panigel, M.: Placental transfer of catecholamines in vitro and in vivo. Am. J. Obstet. Gynecol. 112, 1068–1075 (1972)
Mirkin, B., Sing, S.: Placental transfer of pharmacologically active molecules. In: Perinatal pharmacology and therapeutics (B.L. Mirkin, ed.). New York, San Francisco and London: Academic Press (1976)
Olson, L., Seiger, A.: Early ontogeny of central monoamine neurons in the rat: Fluorescence histochemical observations. Z. Anat. Entwicklungsgesch. 137, 301–316 (1972)
Payne, G.S., Deuchar, E.M.: An in vitro study of functions of embryonic membranes in the rat. J. Embryol. Exp. Morphol. 27, 533–542 (1972)
Pearse, A.G.E.: The cytochemistry and ultrastructure of polypeptide hormone — producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J. Histochem. Cytochem. 17, 303–313 (1969)
Petry, G., Zuleger, S.: Vergleichende Studie über den Bau der Eihüllen der Ratte. Z. Zellforschg. 47, 683–712 (1958)
Saarikoski, S.: Fate of noradrenaline in the human foetoplacental unit. Acta Physiologica Scand. Suppl. 421 (1974)
Schlumpf, M., Lichtensteiger, W., Shoemaker, W.J., Bloom, F.E.: Catecholamines in early development. In: Catecholamines: Basic and clinical Frontiers (E. Usdin, ed.) Oxford: Pergamon Press (in Press)
Seiger, A., Olson, L.: Late prenatal ontogeny of central monoamine neurons in the rat. Fluorescence histochemical observations. Zeitschr. Anat. Entwickl.-Gesch. 140, 281–318 (1973)
Slade, B.S.: An attempt to visualize protein transmission across the rabbit visceral yolk sac. J. Anat. 107, 531–545 (1970)
Wild, A.E.: Protein transmission across the rabbit foetal membranes. J. Embryol. Exp. Morphol. 24, 313–330 (1970)
Wislocki, G.B., Padykula, H.A.: Reichert's membrane and the yolk sac of the rat investigated by histochemical means. Am. J. Anat. 92, 117–153 (1953)
Wislocki, G.B., Deane, H.W., Dempsey, E.W.: The histochemistry of rodent's placenta. Am. J. Anat. 78, 281–344 (1946)
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Supported by grants from SNSF 3.231-0.77, Hartmann Müller Stiftung and Bruner Foundation
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Schlumpf, M., Lichtensteiger, W. Catecholamines in the yolk sac epithelium of the rat. Anat Embryol 156, 177–187 (1979). https://doi.org/10.1007/BF00300013
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DOI: https://doi.org/10.1007/BF00300013