Summary
HRS60.1, a monomer unit (184 bp) of a highly repeated nuclear DNA sequence of Nicotiana tabacum, has been cloned and sequenced. Following BamHI digestion of tobacco DNA, Southern hybridization with HRS60.1 revealed a ladder of hybridization bands corresponding to multiples of the basic monomer unit. If the tobacco DNA was digested with restriction endonucleases which have no target site in HRS60.1, the larger part of DNA homologous to HRS60.1 remained as uncleaved “relic” DNA. These results suggest a tandem arrangement of this DNA repeat unit. Four other clones of tobacco nuclear DNA cross-hybridized with HRS60.1, thus forming a “HRS60-family”. Sequencing their inserts has shown their strong mutual homology. HRS60-family comprised about 2% of the nuclear genome of N. tabacum. Computer comparisons with other tandem plant-repeated DNA sequences could not detect any other homologous sequence.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Appels R, Dennis ES, Smyth DR, Peacock WJ (1981) Two repeated DNA sequences from the heterochromatic region of rye (Secale cereale) chromosomes. Chromosoma 84:265–277
Barnes SR, James AM, Jamieson G (1985) The organisation, nucleotide sequence, and chromosomal distribution of a satellite DNA from Allium cepa. Chromosoma 92:185–192
Bedbrook JR, O'Dell M, Thompson RD, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560
Benslimane AA, Dron M, Hartmann C, Rode A (1986) Small tandemly repeated sequences of higher plants likely originate from a tRNA gene. Nucleic Acids Res 14:8111–8119
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Brennicke A, Hemleben V (1983) Sequence analysis of the cloned Cucumis melo highly repetitive satellite DNA. Z Naturforsch Teil C 38:1062–1065
Capesius I (1983) Sequence of the cryptic satellite DNA from the plant Sinapis alba. Biochim Biophys Acta 739:276–280
Deumling B (1981) Sequence arrangement of a highly methylated satellite DNA of a plant Scilla: a tandemly repeated inverted repeat. Proc Natl Acad Sci USA 78:338–342
Flavell RB (1983) Repeated sequences and genome architecture. In: Cifferi O, Dure III L (eds) Structure and functions of plant genomes. Plenum Press, New York, pp 1–14
Grellet F, Delcasso D, Panabieres F, Delseny M (1986) Organization and evolution of a higher plant alphoid-like satellite DNA sequence. J Mol Biol 187:495–507
Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–580
Karlovská L, Brzobohatý B, Reich J, Koukalová B, Bezděk M (1987) Transfer of Drosophila melanogaster transposable genetic element mdg-4 into plant cells. Folia Biol (Prague) 33:40–49
Kato A, Yakura K, Tanifuji S (1984) Sequence analysis of Vicia faba repeated DNA, the FokI repeat element. Nucleic Acids Res 12:6415–6426
Leclerk RF, Siegel A (1987) Characterization of repetitive elements in several Cucurbita species. Plant Mol Biol 8:497–507
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Martinez-Zapater JM, Estelle MA, Somerville OR (1986) A highly repeated DNA sequence in Arabidopsis thaliana. Mol Gen Genet 204:417–423
Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Peacock W, Dennis ES, Rhoades MM, Pryor AJ (1981) Highly repeated DNA sequence limited to knob heterochromatin in maize. Proc Natl Acad Sci USA 78:4490–4494
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Thomas P (1980) Hybridization of denaturated DNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77:5201–5205
Walbot V, Goldberg R (1979) Plant genome organization and its relationship to classical plant genetics. In: Hall TC, Davies J (eds) Nucleic acids in plants. CRC Press, Boca Raton/FL, pp 3–40
Wu T, Wu R (1987) A new rice repetitive DNA shows sequence homology to both 5S and tRNA. Nucleic Acids Res 15:5913–5923
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp 18 and pUC19 vectors. Gene 33:103–119
Author information
Authors and Affiliations
Additional information
Communicated by Yu. Gleba
Rights and permissions
About this article
Cite this article
Koukalová, B., Reich, J., Matyášek, R. et al. A BamHI family of highly repeated DNA sequences of Nicotiana tabacum . Theoret. Appl. Genetics 78, 77–80 (1989). https://doi.org/10.1007/BF00299757
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00299757