Abstract
Clostridium thermocellum is an anaerobic thermophilic bacterium that produces enthanol from cellulosic substrates. When the organism was grown in continuous culture at dilution rates ranging from 0.04 to 0.25 h-1, growth yields on cellobiose were higher than on glucose, and even higher yields were observed on cellotetraose. However, differences in bacterial yield were much greater at slow growth rates, and it appeared that glucose-grown cells had a fourfold higher (0.41 g substrate/g protein/h) maintenance energy requirement than cellobiose-grown cultures. Cellobiose and glucose were co-utilized in dual substrate continuous culture, and this was in contrast to batch culture experiments which indicated that the organism preferred the disaccharide. These experiments demonstrate that carbohydrate utilization patterns in continuous culture are different from those in batch culture and that submaximal growth rates affect substrate preference and bioenergetic parameters. The mechanisms regulating carbohydrate use may be different in batch versus continuous culture.
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Literature Cited
Alexander JK (1968) Purification and specificity of cellobiose phosphorylase from Clostridium thermocellum. J Biol Chem 243:2899–2904
Alexander JK (1972) Cellodextrin phosphorylase from Clostridium thermocellum. Methods Enzymol 28:948–953
Bailey RW (1958) The reaction of pentoses with anthrone. Biochem J 68:669–674
Begiun P, Aubert J-P (1994) The biological degradation of cellulose. FEMS Microbiol Rev 13:25–58
Cotta MA, Russell JB (1982) Effect of peptides and amino acids on efficiency of ruminal bacterial protein synthesis in continuous culture. J Dairy Sci 65:226–234
Felix CR, Ljungdahl LG (1993) The cellulosome: the exocellular organelle of Clostridium. Annu Rev Microbiol 47:791–819
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Miller GL (1963) Cellodextrins. Methods Carbohydr Chem 3:134–139
Ng TK, Zeikus JG (1982) Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostridium thermohydrosulfuricum. J Bacteriol 150:1391–1399
Nochur SV, Roberts MF, Demain AL (1990) Mutation of Clostridium thermocellum in the presence of certain carbon sources. FEMS Microbiol Lett 71:199–204
Pirt SJ (1965) The maintenance energy of bacteria in growing cultures. Proc R Soc London Ser B 163:224–231
Russell JB, Baldwin RL (1978) Substrate preferences in rumen bacteria: evidence of catabolite regulatory mechanisms. Appl Environ Microbiol 36:319–329
Schafer ML, King KW (1965) Utilization of cellulose oligosac-charides by Cellvibrio gilvus. J Bacteriol 89:113–116
Strobel HJ, Dawson KA (1993) Xylose and arabinose utilization by the ruminal bacterium Butyrivibrio fibrisolvens. FEMS Microbiol Lett 113:291–296
Strobel HJ, Dawson KA, Caldwell FJ (1994) Cellodextrin and glucose utilization by the thermophilic bacterium Clostridium thermocellum. General meeting, Am. Soc. Microbiol., Las Vegas, Nevada, May 24–27, 1994
Thurston B, Dawson KA, Strobel HJ (1993) Cellobiose versus glucose utilization by the ruminal bacterium Ruminococcus albus. Appl Environ Microbiol 59:2631–2637
Van Kessel JS, Russell JB (1992) Energetics of arginine and lysine transport by whole cells and membrane vesicles of strain SR, a monensin-sensitive ruminal bacterium. Appl Environ Microbiol 58:969–975
Wiegel J, Ljungdahl LG (1986) The importance of thermophilic bacteria in biotechnology. Crit Rev Biotechnol 3:39–108
Zeikus JG (1980) Chemical and fuel production by anaerobic bacteria. Annu Rev Microbiol 34:423–464
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Published with the approval of the Director of the Kentucky Agricultural Experiment Station as journal article no. 95-07-064.
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Strobel, H.J. Growth of the thermophilic bacterium Clostridium thermocellum in continuous culture. Current Microbiology 31, 210–214 (1995). https://doi.org/10.1007/BF00298375
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DOI: https://doi.org/10.1007/BF00298375