Abstract
Two new genes, palH and palI, where mutations mimic the effects of acidic growth pH have been identified in Aspergillus nidulans. A palH mutation is phenotypically indistinguishable from mutations in the palA, palB, palC, and palF genes, whereas palI mutations differ only in that they allow some growth at pH 8. Mutations in palA, B, C, F, and H are epistatic to a palI mutation and the significance of this epistasis is discussed. Additionally, palE and palB mutations have been shown to be allelic. Thus, a total of six genes where mutations mimic acidic growth conditions has been identified.
References
Arst HN Jr (1988) Localisation of several chromosome I genes of Aspergillus nidulans: implications for mitotic recombination. Mol Gen Genet 213:545–547]
Arst HN Jr, Cove DJ (1973) Nitrogen metabolite repression in Aspergillus nidulans. Mol Gen Genet 126:111–141
Arst HN Jr, Tollervey DW, Sealy-Lewis HM (1982) A possible regulatory gene for the molybdenum-containing cofactor in Aspergillus nidulans. J Gen Microbiol 128:1083–1093
Caddick MX, Brownlee AG, Arst HN Jr (1986) Regulation of gene expression by pH of the growth medium in Aspergillus nidulans. Mol Gen Genet 203:346–353
Clutterbuck AJ (1974) Aspergillus nidulans In: King RC (ed) Handbook of genetics, vol I. Plenum, New York, pp 447–510
Clutterbuck AJ (1993) Aspergillus nidulans In: O'Brien SJ (ed) Genetic maps. Locus maps of complex genomes, vol 3 (6th edn) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 3.71–3.84
Cove DJ (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta 113:51–56
Cove DJ (1976) Chlorate toxicity in the fungus Aspergillus nidulans. Studies of mutants altered in nitrate assimilation. Mol Gen Genet 146:147–159
Dorn G (1965) Genetic analysis of the phosphatases in Aspergillus nidulans. Genet Res 6:13–26
Dorn GL (1967) A revised map of the eight linkage groups of Aspergillus nidulans. Genetics 56:619–631
Espeso EA, Tilburn J, Arst HN Jr, Peñalva MA (1993) pH regulation is a major determinant in expression of a fungal penicillin biosynthetic gene. EMBO J 12:3947–3956
Hall HK, Karem KL, Foster JW (1994) Molecular responses of microbes to environmental pH stress. Adv Microb Physiol, in press
Käfer E (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19:33–131
Kudla B, Caddick MX, Langdon T, Martinez-Rossi NM, Bennett CF, Sibley S, Davies RW, Arst HN Jr (1990) The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger. EMBO J 9:1355–1364
McCully KS, Forbes E (1965) The use of p-fluorophenylalanine with master strains of Aspergillus nidulans for assigning genes to linkage groups. Genet Res 6:352–359
Olson ER (1993) Influence of pH on bacterial gene expression. Mol Microbiol 8:5–14
Rossi A, Arst HN Jr (1990) Mutants of Aspergillus nidulans able to grow at extremely acidic pH acidify the medium less than wild type when grown at more moderate pH. FEMS Microbiol Lett 66:51–53
Shah AJ, Tilburn J, Adlard MW, Arst HN Jr (1991) pH regulation of penicillin production in Aspergillus nidulans. FEMS Microbiol Lett 77:209–212
Stankovich M, Platt A, Caddick MX, Langdon T, Shaffer PM, Arst HN Jr (1993) C-terminal truncation of the transcriptional activator encoded by areA in Aspergillus nidulans results in both loss-of-function and gain-of-function phenotypes. Mol Microbiol 7:81–87
Author information
Authors and Affiliations
Additional information
Communicated by C. A. M. J. J. van den Hondel
Rights and permissions
About this article
Cite this article
Arst, H.N., Bignell, E. & Tilburn, J. Two new genes involved in signalling ambient pH in Aspergillus nidulans . Molec. Gen. Genet. 245, 787–790 (1994). https://doi.org/10.1007/BF00297286
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00297286