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Activation of mouse ribosomal RNA genes at the 2-cell stage

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Summary

The Ag-AS method, developed by Goodpasture and Bloom (1975) stains transcriptionally active nucleolus organizer regions (NORs) on the chromosomes and in the interphase nuclei. Metaphases and interphase nuclei of early mouse embryos (unfertilized eggs, pronucleus stages, 2-, 4-, 8-cell stages, and morulae) were subjected to silver-staining. First staining of a single chromosome bearing an NOR was observed at the 2-cell stage. At the 4-cell stage 4–6 chromosomes, and at the 8-cell stage invariably all the 6 chromosomes known to bear NORs, respond positively to silver-staining. These results indicate that during mouse embryogenesis ribosomal RNA genes start to function at the 2-cell stage. The polar body does not respond to silver-staining, which supports the view that the polar body genome remains inactive.

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References

  • Bernstein, R. M., Mukherjee, B. B.: Cytoplasmic control of nuclear activity in preimplantation mouse embryos. Develop. Biol. 34, 47–65 (1973)

    PubMed  Google Scholar 

  • Bigger, T. R. L., Savage, J. R. K.: Location of nucleolar organizing regions on the chromosomes of the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus). Cytogenet. Cell Genet. 16, 495–504 (1976)

    PubMed  Google Scholar 

  • Bloom, S. E., Goodpasture, C.: An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes. Hum. Genet. 34, 199–206 (1976)

    PubMed  Google Scholar 

  • Bramwell, M. E., Handmaker, S. D.: Ribosomal RNA synthesis in human-mouse hybrid cells. Biochim. Biophys. Acta 232, 580–583 (1971)

    PubMed  Google Scholar 

  • Brinster, R. L.: Parental glucose phosphate isomerase activity in three-day mouse embryos. Biochem. Genet. 9, 187–191 (1973)

    PubMed  Google Scholar 

  • Brown, D. D., Littna, E.: RNA synthesis during the development of Xenopus laevis, the South African clawed toad. Mol. Biol. 8, 669–687 (1964)

    Google Scholar 

  • Chapman, V. M., Adler, D., Labarca, C. Wudl, L.: Genetic variation of β-glucuronidase expression during early embryogenesis. Ciba Found. Symp. 40 (new series), 124–131 (1976)

    Google Scholar 

  • Church, R. B., Schultz, G. A.: Differential gene activity in the pre- and postimplantation mammalian embryo. Curr. Top. Dev. Biol. 8, 179–202 (1974)

    PubMed  Google Scholar 

  • Cooper, H. L.: Studies on RNA metabolism during lymphocyte activation. Transplant. Rev. 11, 3–38 (1972)

    PubMed  Google Scholar 

  • Croce, C. M., Talavera, A., Basilico, C. Miller, O. J.: Suppression of production of mouse 28S ribosomal RNA in mouse-human hybrids segregating mouse chromosomes. Proc. nat. Acad. Sci. (Wash.) 74, 694–697 (1977)

    Google Scholar 

  • Ellem, K. A., Gwatkin, R. B. L.: Patterns of nucleic acid synthesis in the early mouse embryo. Develop. Biol. 18, 311–330 (1968)

    PubMed  Google Scholar 

  • Eliceiri, G. L., Green, H.: Ribosomal RNA synthesis in human-mouse hybrid cells. J. molec. Biol. 41, 253–260 (1969)

    PubMed  Google Scholar 

  • Engel, W., Franke, W.: Maternal storage in the mammalian oocyte Curr. Top. Pathol. 62, 29–52 (1976)

    PubMed  Google Scholar 

  • Epstein, C. J.: Expression of the mammalian X-chromosome before and after fertilization. Science 175, 1467–1468 (1972)

    PubMed  Google Scholar 

  • Epstein, C. J.: Gene expression and macromolecular synthesis during preimplantation embryonic development. Biol. Reprod. 12, 82–105 (1975)

    PubMed  Google Scholar 

  • Erickson, R. P.: Androgen-modified expression compared with Y-linkage of male specific antigen. Nature 265, 59–61, (1977)

    PubMed  Google Scholar 

  • Galdieri, M., Monesi, V.: Ribosomal RNA in mouse spermatocytes. Exp. Cell Res. 85, 287–295 (1974)

    PubMed  Google Scholar 

  • Goodpasture, C., Bloom, S. E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma (Berl.) 53, 37–50 (1975)

    Google Scholar 

  • Henderson, A. S., Eicher, E. M., Yu, M. T., Atwood, K. C.: The chromosomal location of ribosomal DNA in the mouse. Chromosoma (Berl.) 49, 155–160 (1974)

    Google Scholar 

  • Hillman, N., Tasca, R. J.: Ultrastructural and autoradiographic studies of mouse cleavage stages. Amer. J. Anat. 126, 151–174 (1970)

    Google Scholar 

  • Howell, W. M., Denton, T. E., Diamond, J. R.: Differential staining of the satellite regions of human acrocentric chromosomes. Experientia (Basel) 31, 260–262 (1975)

    Google Scholar 

  • Knowland, J., Graham, C.: RNA synthesis at the two-cell stage of the mouse development. J. Embryol. exp. Morph. 27, 167–176 (1972)

    PubMed  Google Scholar 

  • Kozak, P. L., Quinn, P. J.: Evidence for dosage compensation of an X-linked gene in the 6-day embryo of the mouse. Develop. Biol. 45, 65–73 (1975)

    PubMed  Google Scholar 

  • Krco, C. J., Goldberg, E. H.: H-Y (male) antigen: detection on eight-cell mouse embryos. Science 193, 1134–1135 (1976)

    PubMed  Google Scholar 

  • Manes, C.: Genetic and biochemical activities in preimplantation embryos. In: The developmental biology of reproduction, C. L. Markert, J. Papaconstantinou, eds., pp 133–163. New York-San Francisco-London: Academic Press 1975

    Google Scholar 

  • Marshall, C. J., Handmaker, S. D., Bramwell, M. E.: Synthesis of ribosomal RNA in synkaryons and heterokaryons formed between human and rodent cells. J. Cell Sci. 17, 307–325 (1975)

    PubMed  Google Scholar 

  • Miller, D. A., Dev, V. G., Tantravahi, R. Miller, O. J.: Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp. Cell Res. 101, 235–243 (1976a)

    PubMed  Google Scholar 

  • Miller, O. J., Miller, D. A., Dev, V. G., Tantravahi, R., Croce, C. M.: Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. Proc. nat. Acad. Sci. (Wash.) 73, 4531–4535 (1976b)

    Google Scholar 

  • Muggleton-Harris, A. L., Johnson, M. H.: The nature and distribution of serologically detectable alloantigens on the preimplantation mouse embryo. J. Embryol. exp. Morph. 35, 59–72 (1976)

    PubMed  Google Scholar 

  • Pikó, L.: Expression of mitochondrial and nuclear genes during early development. In: The early development of mammals, M. Balls, A. E. Wild, eds., pp. 167–187. London-New York-Melbourne: Cambridge University Press 1975

    Google Scholar 

  • Purtell, M. J., Anthony, D. D.: Changes in ribosomal RNA processing paths in resting and phytohemagglutinin-stimulated guinea pig lymphocytes. Proc. nat. Acad. Sci. (Wash.) 72, 3315–3319 (1975)

    Google Scholar 

  • Tantravahi, R., Miller, D. A., Dev, V. G., Miller, O. J.: Detection of nucleolus organizer regions in chromosomes of human, chimpanzee, gorilla, orang-utan and gibbon. Chromosoma (Berl.) 56, 15–27, (1976)

    Article  Google Scholar 

  • Tarkowski, A. K.: An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 5, 394–400 (1966)

    Google Scholar 

  • Tata, J. R.: Hormonal regulation of growth and protein synthesis. Nature 219, 331–337 (1968)

    PubMed  Google Scholar 

  • Tres, L. L.: Nucleolar RNA synthesis of meiotic prophase spermatocytes in the human testis. Chromosoma (Berl.) 53, 141–151 (1975)

    Google Scholar 

  • Wolf, U., Engel, W.: Gene activation during early development of mammals. Humangenetik 15, 99–118 (1972)

    PubMed  Google Scholar 

  • Woodland, H. R., Graham, C. F.: RNA synthesis during early development of the mouse. Nature 221, 327–332 (1969)

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut für Humangenetik und Anthropologie, Albertstraße 11, D-7800, Freiburg, Germany

    W. Engel & Maria T. Zenzes

  2. Abteilung für Humangenetik der Universität, Oberer Engelsberg, D-6900, Ulm, Germany

    M. Schmid

Authors
  1. W. Engel
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  2. Maria T. Zenzes
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  3. M. Schmid
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Engel, W., Zenzes, M.T. & Schmid, M. Activation of mouse ribosomal RNA genes at the 2-cell stage. Hum Genet 38, 57–63 (1977). https://doi.org/10.1007/BF00295808

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  • DOI: https://doi.org/10.1007/BF00295808

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