Summary
A study of linkage between Becker muscular dystrophy and four X chromosome-specific DNA polymorphisms in 17 kindreds has indicated that this gene is located in Xp, as already anticipated by single pedigree analysis. In particular the DXS43 and DXS9 loci, identified by probes D2 and RC8, respectively, are closely linked to each other and are both located at approximately 15 cM from the Becker locus. These linkage data, together with the previously established linkage between Becker and the DXS7 locus identified by probe L 1.28, indicate that the Becker gene is located in the same region where Duchenne has been mapped and also yield information about relative genetic distances among different DNA polymorphisms of the X chromosome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aldridge J, Kunkel L, Bruns G, Tantravahi U, Laland M, Brewster T, Moreau E (1984) A strategy to reveal high frequency RFLP along the human X chromosome. Am J Hum Genet 36:546–564
Brooke MH, Griggs RC, Mendel JR, Fenichel GM, Schumate J, Pellegrino RI (1981) Clinical trials in Duchenne dystrophy: The design of the protocol. Muscle Nerve 4:186–197
Davies KE, Pearson PL, Harper PS, Murray JM, O'Brien T, Sarfarazi M, Williamson R (1983) Linkage analysis of two cloned DNA sequences flanking the Duchenne muscular dystrophy locus on the short arm of the human X chromosome. Nucleic Acids Res 11:2303–2312
Drayna D, Davies K, Hartley D, Mandel JL, Camerino G, Williamson R, White R (1984) Genetic mapping of the human X chromosome by using restriction fragment length polymorphisms. Proc Natl Acad Sci USA 81:2836–2839
Emery AEH (1976) Methodology in medical genetics. An introduction to statistical methods. Churchill Livingstone, Edinburgh London
Greenstein RM, Reardon MP, Chan TS, Middleton AB, Mulivor RA, Greene RE, Coriell LL (1980) An (X;11) translocation in a girl with Duchenne muscular dystrophy. Cytogenet Cell Genet 27:268
Kingston HM, Thomas NST, Pearson PL, Sarfarazi M, Harper PS (1983) Genetic linkage between Becker muscular dystrophy and a polymorphic DNA sequence on the short arm of the X chromosome. J Med Genet 20:255–258
Kingston HM, Sarfarazi M, Thomas NST, Harper PS (1984) Localisation of the Becker muscular dystrophy gene on the short arm of the X chromosome by linkage to cloned DNA sequences. Hum Genet 67:6–17
Kunkel LM, Smith KD, Boyer SH, Borgaonkar DS, Wachtel SS, Miller OJ, Breg WR, Jones HW Jr, Rary JM (1977) Analysis of human Y-chromosome specific reiterated DNA in chromosome variants. Proc Natl Acad Sci USA 74:1245–1249
Murray JM, Davies KE, Harper PS, Meredith L, Mueller CR, Williamson R (1982) Linkage relationship of a cloned DNA sequence on the short arm of the X chromosome to Duchenne muscular dystrophy Nature 300:69–71
Page D, De Martinville B, Barker D, Whyman A, Ahite R, Francke U, Botstein D (1982) Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes. Proc Natl Acad Sci USA 79:5352–5356
Pearson PL, Wieacher P, Backer B, Prins HA (to be published) Clin Genet
Roncuzzi L, Fadda S, Mochi M, Prosperi L, Sangiorgi S, Santamaria R, Sbarra D, Besana D, Morandi L, Rocchi M, Romeo G (1985) Mapping of X-linked Becker muscular dystrophy through crossovers identified by DNA polymorphisms and by haplotype characterization in somatic cell hybrids. Am J Hum Genet 37:407–417
Skinner R, Smith C, Emery AH (1974) Linkage between the loci for benign (Becker type) X-borne muscular dystrophy and deutan colour blindness. J Med Genet 11:317–320
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Verellen-Dumoulin C, Freund M, De Meyer R, Laterre C, Frédéric J, Thompson MW, Markovic VD, Worton RG (1984) Expression of an X-linked muscular dystrophy in a female due to translocation involving Xp21 and non-random inactivation of the normal X chromosome. Hum Genet 67:115–119
Zatz M, Itskan SB, Sanger R, Frota-Pessoa O, Saldanha PH (1974) New linkage data for the X-linked types of muscular dystrophy and G6PD variants, colour blindness and Xg blood groups. J Med Genet 11:321–327
Zatz M, Vianna Morgante AM, Campos P, Diamant AJ (1981) Translocation (X;6) in a female with Duchenne muscular dystrophy: Implications for the localization of the DMD locus. J Med Genet 18:442–447
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fadda, S., Mochi, M., Roncuzzi, L. et al. Definitive localization of Becker muscular dystrophy in Xp by linkage to a cluster of DNA polymorphisms (DXS43 and DXS9). Hum Genet 71, 33–36 (1985). https://doi.org/10.1007/BF00295664
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00295664