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Nucleotide polymorphism in chloroplast dna of Nicotiana debneyi

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Summary

EcoR1 restriction endonuclease analysis of chloroplast DNA isolated from several distinct populations of Nicotiana debneyi has revealed a naturally occurring polymorphism. The chloroplast DNA of seven of the nine populations analysed possessed an additional EcoRl site. The origin of the additional restriction endonuclease fragments was confirmed by hybridisation of [32 P]-cRNA to fractionated EcoRl restricted chloroplast-DNA fragments adsorbed to nitrocellulose filters. Reciprocal f1 hybrids between plants carrying the variant chloroplast-DNA's confirmed maternal inheritance of chloroplast-DNA.

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Literature

  • Appels, R.; Driscoll, C.; Peacock, W.J.: Heterochromatin and highly repeated DNA sequences in rye (Secale cereale). Chromosoma 70, 67–89 (1978)

    Google Scholar 

  • Atchison, B.A.; Whitfield, P.R.; Bottomley, W.: Comparison of chloroplast DNAs by specific fragmentation with EcoRl endonuclease. Molec. gen. Genet. 148, 263–269 (1976)

    Google Scholar 

  • Belliard, G.; Pelletier, G.; Vedel, F.; Quetier, F.: Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Molec. gen. Genet. 165, 231–237 (1978)

    Google Scholar 

  • Bogorad, L.: Evolution of organelles and eukaryotic genomes. Science 188, 891–898 (1975)

    Google Scholar 

  • Brewer, G.J.; Sing, C.F.: An introduction to isozyme techniques. London, New York: Acad. Press 1970

    Google Scholar 

  • Brown, A.H.D.; Nevo, E.; Zohary, D.; Dagan, O.: Genetic variation in natural populations of wild barley (Hordeum spontaneum). Genetica 49, 97–108 (1978)

    Google Scholar 

  • Burbidge, N.T.: The Australian species of Nicotiana L. (Solanaceae). Austr. J. Bot. 8, 342–380 (1960)

    Google Scholar 

  • Buzzo, K.; Fouts, D.L.; Wolstenholme, D.R.: EcoRl cleavage site variants of mitochondrial DNA molecules from rats. Proc. nat. Acad. Sci. (Wash) 75, 909–913 (1978)

    Google Scholar 

  • Chen, K.; Johal, S.; Wildman, S.G.: Phenotypic markers for chloroplast DNA genes in higher plants and their use in biochemical genetics. In: Nucleic acids and protein in plants (ed. Bogorad, L.; Weil, J.H.), pp. 183–194. New York: Plenum Press 1977

    Google Scholar 

  • Frankel, R.; Scowcroft W.R.; Whitfeld, P.R.: Chloroplast DNA variation in male-sterile lines of Nicotiana. Molec. gen. Genet. 169, 129–135 (1979)

    Google Scholar 

  • Goodspeed, T.H.: The Genus Nicotiana. Chronica Botanica (Waltham, Mass) 1954

  • Holder, A.A.: Peptide mapping of the ribulose bisphosphate carboxylase large subunit from the genus Oenothera. Carlsberg. Res. Comm. 43, 391–399 (1978)

    Google Scholar 

  • Kung, S.: Expression of chloroplast genomes in higher plants. Ann. Rev. Plant Physiol. 28, 401–437 (1977)

    Google Scholar 

  • Langridge, J.; Langridge, P.; Bergquist, P.L.: Extraction of nucleic acids from agarose gels. Nucleic Acid. Res. (1979, in press)

  • Levings, C.S.; Pring, D.R.: Restriction endonuclease analysis of mitochondrial DNA from normal and Texas cytoplasmic malesterile maize. Science 193, 158–160 (1976)

    Google Scholar 

  • Potter, S.S.; Newbold, J.E.; Hutchison, C.A.; Edgell, M.H.: Specific cleavage analysis of mammalian mitochondrial DNA. Proc. nat. Acad. Sci. (Wash) 72, 4496–4500 (1975)

    Google Scholar 

  • Pring, D.R.; Levings, C.S.: Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89, 212–136 (1978)

    Google Scholar 

  • Quetier, F.; Vedel, F.: Heterogeneous populations of mitochondrial DNA molecules in higher plants. Nature 268, 365–368 (1977)

    Google Scholar 

  • Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. molec. Biol. 98, 503–518 (1975)

    Google Scholar 

  • Symons, R.H.: Synthesis of [α-32P] ribo- and deoxyribonucleoside 51 -triphosphates. Methods in enzymology 29, 102–115 (1974)

    Google Scholar 

  • Upholt, W.B.: Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res. 4, 1257–1265 (1977)

    Google Scholar 

  • Vedel, F.; Quetier, F.; Bayen, M.: Specific cleavage of chloroplast DNA from higher plants by EcoRl restriction nuclease. Nature 263, 440–442 (1976)

    Google Scholar 

  • Wettstein, D. von; Poulsen, C.; Holder, A.A.: Ribulose-l,5-bisphosphate carboxylase as a nuclear and chloroplast marker. Theor. Appl. Genet. 53, 193–197 (1978)

    Google Scholar 

  • Whitfeld, P.R.; Spencer, D.: Buoyant density of tobacco and spinach chloroplast DNA. Biochim. biophys. Acta 157, 333–343 (1968)

    Google Scholar 

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Authors and Affiliations

  1. CSIRO Division of Plant Industry, Canberra City, Australia

    W. R. Scowcroft

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  1. W. R. Scowcroft
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Communicated by G. Melchers and D. von Wettstein

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Scowcroft, W.R. Nucleotide polymorphism in chloroplast dna of Nicotiana debneyi . Theoret. Appl. Genetics 55, 133–137 (1979). https://doi.org/10.1007/BF00295439

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  • DOI: https://doi.org/10.1007/BF00295439

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