Abstract
A gene corresponding to a cDNA clone, SE60, encoding a low molecular weight sulfur-rich protein in soybean seeds was isolated from a soybean genomic library and characterized at the nucleotide level. The SE60 gene is interrupted by an intervening sequence of 694 by in size. The 5′ flanking region of the gene contained various regulatory sequences such as the RY repeat and CACA elements found in other seed protein genes of legumes. The SE60 gene encoded a pre-protein of 75 amino acids, having a signal sequence of 28 amino acids at the N-terminus. The mature protein of 47 amino acids was basic and cysteine-rich. Northern blot analysis suggested that the SE60 gene is expressed in a tissue-specific and developmentally regulated manner during soybean seed development. The SE60 genes form a small multigene family composed of about four members in the soybean genome.
This is a preview of subscription content, log in via an institution to check access.
References
Bloch C Jr, Richardson M (1991) A new family of small (5 kDa) protein inhibitors of insect α-amylases from seeds of sorghum (Sorghum bicolor (L.) Moench) have sequence homologies with wheat γ-purothionins. FEBS Lett 279:101–104
Bohlmann H (1994) The role of thionins in plant protection. Crit Rev Plant Sci 13:1–16
Bohlmann H, Apel K (1991) Thionins. Annu Rev Plant Physiol Plant Mol Biol 42:227–240
Choi Y, Choi YD, Lee JS (1993) Nucleotide sequence of a cDNA encoding a low molecular weight sulfur-rich protein in soybean seeds. Plant Physiol 101:699–700
Colilla FJ, Rocher A, Mendez E (1990) γ-Purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm. FEBS Lett 270:191–194
Derbyshire E, Wright DS, Boulter D (1976) Legumin and vicillin, storage proteins of legume seeds. Phytochemistry 15:3–24
Dickinson CD, Evans RP, Nielsen NC (1988) RY repeats are conserved in the 5′-flanking regions of legume seed-protein genes. Nucleic Acids Res 16:371
Goldberg RB (1986) Regulation of plant gene expression. Philos Trans R Soc Lond Series B 314:343–353
Goldberg RB, Barker SJ, Perez-Grau L (1989) Regulation of gene expression during plant embryogenesis. Cell 56:149–160
Gu Q, Kawata EE, Morse M-J, Wu H-M, Cheung AY (1992) A flower-specific cDNA encoding a novel thionin in tobacco. Mol Gen Genet 234:89–96
Ishibashi N, Yamauchi D, Minamikawa T (1990) Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination. Plant Mol Biol 15:59–64
Jofuku KD, Okamura JK, Goldberg RB (1987) Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region. Nature 328:734–737
Kim CH, Lee SY, Choi YD (1992) Molecular cloning of genomic DNA encoding the soybean glycinin subunit A2B1a. Mol Cells 2:155–163
Meinke DW, Chen J, Beachy RN (1981) Expression of storage-protein genes during soybean seed development. Planta 153:130–139
Oeda K, Salinas J, Chua N-H (1991) A tobacco bZip transcription activator (TAF-1) binds to a G box-like motif conserved in plant genes. EMBO J 10:1793–1802
Stiekema WJ, Heidekamp F, Dirkse WG, van Beckum J, de Haan P, ten Bosch C, Louwerse JD (1988) Molecular cloning and analysis of four potato tuber mRNAs. Plant Mol Biol 11:255–269
Von Heijne G (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14:4683–4690
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Choi, Y., Ahn, J.H., Choi, Y.D. et al. Tissue-specific and developmental regulation of a gene encoding a low molecular weight sulfur-rich protein in soybean seeds. Molec. Gen. Genet. 246, 266–268 (1995). https://doi.org/10.1007/BF00294691
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00294691