Summary
The green alga Scenedesmus obliquus was immobilized in Ca-alginate beads. The cell growth after immobilization was studied by cell counting. The nitrite uptake was not affected by immobilization, except that a longer lag phase was observed in immobilized cells than in free ones. That result could be due to a barrier effect of the matrix against nitrite diffusion inside the beads. The treatment of cells by glycerol prior to their immobilization in a batch reactor induced an increase of nitrite uptake by the cells. This effect disappeared after a few runs. The glycerol effect on specific rates seemed also to decrease when the number of immobilized cells increased. This decrease can be related to the decrease of light efficiency as well as substrate accessibility when a high cell concentration was used. Several alternating runs of Tris-HCl buffer containing nitrite growth medium depleted in combined nitrogen were tested. Cellular growth occurred inside the beads up to a maximum followed by a decrease of cell number in the beads.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avissar YJ (1985) Induction of nitrate assimilation in the cyanobacterium Anabaena variabilis. Physiol Plant 63:105–108
Barbotin JN, Thomasset B (1980) Immobilized organelles and whole cells into protein foam structures: scanning and transmission electron microscope observations. Biochimie 62:359–366
Baudet C, Barbotin JN, Guespin-Michel J (1983) Growth and sporulation of Bacillus subtilis cells. Appl Environ Microbiol 45:297–301
Brouers N, Collard F, Jeanfils J, Loudèche R (1982) Long term stabilization of photobiological activities of immobilized algae. Photoproduction of hydrogen by immobilized “adapted” Scenedesmus cells. In: Hall DO, Paltz W (eds) Photochemical, photoelectrochemical and photobiological processes. Vol. 2, Reidel Publishing Company, Dordrecht, pp 171–178
Butler WL (1978) Energy distribution in the photochemical apparatus of photosynthesis. Ann Rev Plant Physiol 29:345–378
Butler WL, Kitajima M (1975) Energy transfer between photo-system II and photosystem I. Biochim Biophys Acta 396:72–85
Cho F, Govindjee (1970) Low temperature (4–77 K) spectros-copy of Chlorella, temperature dependence of energy transfer efficiency. Biochim Biophys 216:139–150
Dhulster P, Barbotin JN, Thomas D (1984) Culture and bioconversion of plasmid-harboring strain of immobilized E. coli. Appl Microbiol Biotechnol 20:87–93
Fukui S, Tanaka A (1982) Immobilized microbial cells. Ann Rev Microbiol 36:145–172
Hattori A (1982) Adaptative formation of nitrate reducing system in Anabaena cylindrica. Plant Cell Physiol 3:371–377
Herrero A, Flores E, Guerrero MG (1981) Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp strain 7119 and Nostoc sp strain 6719. J Bact 145:175–180
Hipkin CR, Syrett PJ (1973) Enzymatic determination of nitrate by use of frozen thawed Chlorella cells. New Phytol 72:47–49
Hipkin CR, Syrette PJ (1977) Nitrate reduction by whole cells of Ankistrodermus braunii and Chlamydomonas reinhardi. New Phytol 79:639–648
Jeanfils J, Collard F (1983) Effect of immobilizing Scenedesmus obliquus cells in a matrix on oxygen evolution and fluorescence properties. Appl Microbiol Biotechnol 17:254–257
Larreta Garde V, Thomasset B, Barbotin JN (1981) Electron microscopy evidence of an immobilized living cell system. Enz Microbiol Technol 3:216–218
Losada M, Guerrero MG (1979) The photosynthetic reduction of nitrate and its regulation. In: Barber J (ed) Photosynthesis in relation to model system. Elsevier, Amsterdam, pp 365–408
Manzano C, Candan P, Gomez-Moreno C, Relimpio AM, Losada M (1976) Ferredoxin dependent photosynthetic reduction of nitrate and nitrite by particles of Anacystis nidulans. Mol Cell Biochem 10:161–169
Mohan RR, Li MN (1975) Nitrate and nitrite reduction by liquid membrane encapsulated whole cells. Biotechnol Bioeng 17:1137–1156
Mohanty P, Braun BZ, Govindjee, Thornber JP (1972) Chlorophyll fluorescence characteristics of system I chlorophyll a protein complex and system II particles at room and liquid nitrogen temperature. Plant Cell Physiol 13:81–91
Murata N (1969) Control of excitation transfer in photosynthesis. Biochim Biophys Acta 189:171–181
Nilsson K, Birnbaum S, Flygare S, Linse L, Schröder V, Jeppson V, Lasson PO, Mosbach K, Brodelius P (1983) A general method for immobilization of cells which preserved viability. Appl Microbiol Biotechnol 17:319–326
Sironval C, Brouers M, Michel JM, Kuiper Y (1968) The reduction of protochlorophyllide into chlorophyllide. Photosynthetica 2:268–287
Snell PD, Snell CT (1949) Colorimetric methods of analysis. Vol. 3. Van Nostrand, New York, pp 804–805
Syrett PJ, Thomas EM (1973) The assay of nitrate reductase in whole cells of Chlorella: strain differences and the effect of cell walls. New Phytol 72:1307–1314
Trainor FR, Rosbowsky FG (1967) Control of unicell formation in a soil Scenedesmus. Canadian J Botany 45:1657–1664
Vennesland B, Guerrero MG (1979) Reduction of nitrate and nitrite. In: Pirson A, Zimmermann MH (eds) Encyclopedia of plant physiology. New series. Vol. 6, Springer Verlag, Berlin, pp 425–434
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jeanfils, J., Thomas, D. Culture and nitrite uptake in immobilized Scenedesmus obliquus . Appl Microbiol Biotechnol 24, 417–422 (1986). https://doi.org/10.1007/BF00294600
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00294600