Abstract
A number of integrational vectors were developed for use as genetic tools in the food-borne pathogen Campylobacter coli. Integration of the plasmids occurred following genetic recombination via a Campbell-like mechanism. For an integrative plasmid containing a DNA fragment internal to the C. coli catalase gene, the insertion was mutagenic and led to a catalase-deficient phenotype. A procedure for generating random mutations in the C. coli chromosome, with these suicide-plasmids, was developed. In addition, the construction and utility of an integrable plasmid for generating transcriptional fusions to a cat reporter gene is described.
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Dickinson, J.H., Grant, K.A. & Park, S.F. Targeted and random mutagenesis of the Campylobacter coli chromosome with integrational plasmid vectors. Current Microbiology 31, 92–96 (1995). https://doi.org/10.1007/BF00294282
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DOI: https://doi.org/10.1007/BF00294282