Abstract
Aerobic mycelium of wild-type Mucor rouxii accumulated about ten times higher amounts of the carotenoid pigment β-carotene when grown continuously in the presence of light than the corresponding cultures grown in the dark. Carotenoid accumulation was dependent on light intensity, with the threshold located at about 10-2 W.m-2. Photocarotenogenesis in complex medium was more efficient with glucose as a carbon source. Carotenoid synthesis by M. rouxii mycelium was unaffected by both retinol acetate and retinal, which are stimulators of carotenogenesis in other zygomycetes. Carotenogenesis was significant in aerobic mycelium but was almost undetectable in anaerobic mycelium as well as in aerobic or anaerobic yeast cells. This suggested an involvement of oxygen in carotenoid synthesis by M. rouxii and the existence of developmental regulation of the expression or operation of the pathway.
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Literature Cited
Avalos J, Bejarano ER, Cerdá-Olmedo E (1993) Photoinduction of carotenoid biosynthesis. Methods Enzymol 214:283–296
Bartnicki-García S (1968) Control of dimorphism in Mucor by hexoses: inhibition of hyphal morphogenesis. J Bacteriol 96: 1586–1594
Bartnicki-García S, Nickerson WJ (1962a) Nutrition, growth and morphogenesis of Mucor rouxii. J Bacteriol 84:841–858
Bartnicki-García S, Nickerson WJ (1962b) Induction of yeast-like development in Mucor by carbon dioxide. J Bacteriol 84:829–840
Bramley PM, Mackenzie A (1988) Regulation of carotenoid biosynthesis. Curr Topics Cell Regul 29:291–343
Cerdá-Olmedo E (1987) Carotene. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp 199–222
Cerdá-Olmedo E (1989) Production of carotenoids with fungi. In: Vandamme E (ed) Biotechnology of vitamins, growth factor and pigment production. Londres: Elsevier Applied Science, pp 27–42
Davies BH (1965) Analysis of carotenoid pigments. In: Goodwin TW (ed) Chemistry and biochemistry of plant pigments. London: Academic Press, pp 489–532
Eslava AP, Alvarez MI, Cerdá-Olmedo E (1974) Regulation of carotene biosynthesis in Phycomyces by vitamin A and β-ionone. Eur J Biochem 48:617–623
Feofilova EP, Bekhtereva MN (1976) Effect of dimitin on carotene production by Blakeslea trispora. Mikrobiologiya 45: 557–558
Goodwin TW (1980) The biochemistry of carotenoids, Vol 1, 2nd ed. Chapman and Hall. London: Academic Press
Govind NS, Cerdá-Olmedo E (1986) Sexual activation of carotenogenesis in Phycomyces blakesleeanus. J Gen Microbiol 132:2775–2780
Harding RW (1974) The effect of temperature on photoinduced carotenoid biosynthesis in Neurospora crassa. Plant Physiol 54:142–147
Harding RW, Shropshire W (1980) Photocontrol of carotenoid biosynthesis. Annu Rev Plant Physiol 31:217–238
López-Díaz I, Cerdá-Olmedo E (1980) Relationship of carotenogenesis to other behavioural and regulatory responses in Phycomyces. Planta 150:134–139
Murillo FJ, Torres-Martínez S, Aragón CMG, Cerdá-Olmedo E (1981) Substrate transfer in carotene biosynthesis in Phycomyces. Eur J Biochem 119:511–516
Orlowski M (1991) Mucor dimorphism. Microbiol Rev 55:234–258
Ortíz-Castellanos ML, Gutiérrez-Corona JF (1988) The sensitive period for light and temperature regulation of sporangiophore development in Phycomyces. Planta 174:305–308
Rau W (1983) Photoregulation of carotenoid biosynthesis. In: Porter JW, Spurgeon SL (eds) Biosynthesis of isoprenoid compounds. New York: John Wiley, pp 123–157
Sypherd PS, Borgia PT, Paznokas JL (1978) The biochemistry of dimorphism in the fungus Mucor. Adv Microb Physiol 18:67–104
Tada M, Shiroishi M (1982) Mechanism of photoregulated carotenogenesis in Rhodotorula minuta I. Photocontrol of carotenoid production. Plant Cell Physiol 23:541–547
Terenzi HF, Storck R (1968) Stimulation of fermentation and yeast-like morphogenesis of Mucor rouxii by phenethyl alcohol. J Bacteriol 97:1248–1261
Thomas DM, Goodwin TW (1967) Studies on carotenogenesis in Blakeslea trispora. I. General observations on synthesis in mated and unmated strains. Phytochemistry 6:355–360
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Mosqueda-Cano, G., Gutiérrez-Corona, J.F. Environmental and developmental regulation of carotenogenesis in the dimorphic fungus Mucor rouxii . Current Microbiology 31, 141–145 (1995). https://doi.org/10.1007/BF00293544
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DOI: https://doi.org/10.1007/BF00293544