Summary
The relative DNA content of individual chromosomes of Secale cereale L. was determined in 25 cells by microdensitometry of Feulgen stained preparations. The correlation value between relative DNA content and relative chromosome length was r=0.61 for all 328 chromosomes measured. However, the correlation coefficients calculated for individual cells as well as for mean values always approached 1. Taking into account the structure of rye chromosomes, this indicates that microdensitometric results may not be accurate when large quantities of heterochromatic DNA sequences are present in analyzed material.
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Literature
Bennett, M.D.; Gustafson, J.P.; Smith, J.B. (1977): Variation in nuclear DNA in the genus Secale. Chromosoma 61, 149–176
Carlson, W.R. (1978): The B chromosome of corn. Ann. Rev. Genet. 12, 5–23
Darvey, N.L.; Gustafson, J.P. (1975): Identification of rye chromosomes in wheat-rye addition lines and triticale by heterochromatin bands. Crop Sci. 15, 239–243
Giraldez, R.; Cermeno, M.C.; Orellana, J. (1979): Comparison of C-banding pattern in the chromosomes of inbred lines and open varieties of rye (Secale cereale L.). Z. Pflanzenzücht. 83, 40–48
Gustafson, J.P. (1976): The evolutionary development of triticale: the wheat-rye hybrid. Evol. Biol. 9, 107–135
Gustafson, J.P. (1982): Cytogenetics of Triticale. In: Cytogenetics of Crop Plants (eds. Swaminathan, M.S.; Gupta, R.K.; Sinha, N.) London: Macmillan (in press)
Gustafson, J.P.; Bennett, M.D. (1976): Preferential selection for wheat-rye substitutions in 42-chromosome triticale. Crop Sci. 16, 688–693
Gustafson, J.P.; Bennett, M.D. (1982): The effect of telomeric heterochromatin from Secale cereale L. on triticale (X Triticosecale Wittmack). I. The influence of several blocks of telomeric heterochromatin on early endosperm development and kernel characteristics at maturity. Can. J. Genet. Cytol. (in press)
Hennen, W.K.; Casperson, T. (1973): Identification of the chromosomes of rye by distribution patterns of DNA. Hereditas 74, 259–272
Jones, J. (1980): Ph. D. thesis. University of Cambridge, England
Jones, R.N.; Rees, H. (1968): The influence of B-chromosomes upon the nuclear phenotype of rye. Chromosoma 24, 158–176
Kranz, A.R.; Goethe, J.W. (1976): Karyotype analysis in meiosis; giemsa banding in the genus Secale L. Theor. Appl. Genet. 46, 101–107
Lima-de-Faria, A. (1952): Pachytene analysis of the chromosome complement of rye. Chromosoma 5, 1–68
Lukaszewski, A.J.; Apolinarska, B.; Lapinski, B.; Sodkiewicz, W. (1980): Chromosome elimination in the Triticum persicum X T. dicoccoides) X Secale cereale F1 hybrids. Hod. Rosl. Aklim. Nasien. 24, 365–372
Merker, A. (1976): The cytogenetic effect of heterochromatin in hexaploid triticale. Hereditas 83, 215–222
Nishikawa, K. (1970): DNA content of the individual chromosomes and genomes in wheat and its relatives. Seiken Ziho 22, 57–65
Pieritz, W.J. (1970): Elimination von Chromosomen in Amphidiploiden Weizen-Roggen-Bastarden (Triticale). Z. Pflanzenzücht. 64, 90–109
Radley, J.M. (1966): DNA content of individual chromosomes of Protomnodon bicolor. Exp. Cell Res. 41, 217–220
Rees, H.; Hutchinson, J. (1973): Nuclear DNA variation due to B chromosomes. Cold Spring Harbor Symp. Quant. Biol. 38, 175–181
Roupakias, D.G.; Kaltsikes, P.J. (1977): The effect of telomeric heterochromatin on chromosome pairing of hexaploid triticale. Can. J. Genet. Cytol. 19, 543–548
Thomas, H.M. (1981): The giemsa C-band karyolypes of six Lolium species. Heredity 46, 263–267
Verma, S.C.; Recs, H. (1974): Giemsa staining and the distribution of heterochromatin in rye chromosomes. Heredity 32, 118–122
Wcimarck, A. (1975): Heterochromatin polymorphism in rye karyotypes as detected by the giemsa C-banding technique. Hereditas 79, 293–300
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Lukaszewski, A.J., Wozna, J., Apolinarska, B. et al. The DNA content of the individual chromosomes of rye. Theoret. Appl. Genetics 62, 145–148 (1982). https://doi.org/10.1007/BF00293347
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DOI: https://doi.org/10.1007/BF00293347