Skip to main content
Log in

The DNA content of the individual chromosomes of rye

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

The relative DNA content of individual chromosomes of Secale cereale L. was determined in 25 cells by microdensitometry of Feulgen stained preparations. The correlation value between relative DNA content and relative chromosome length was r=0.61 for all 328 chromosomes measured. However, the correlation coefficients calculated for individual cells as well as for mean values always approached 1. Taking into account the structure of rye chromosomes, this indicates that microdensitometric results may not be accurate when large quantities of heterochromatic DNA sequences are present in analyzed material.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature

  • Bennett, M.D.; Gustafson, J.P.; Smith, J.B. (1977): Variation in nuclear DNA in the genus Secale. Chromosoma 61, 149–176

    Google Scholar 

  • Carlson, W.R. (1978): The B chromosome of corn. Ann. Rev. Genet. 12, 5–23

    Google Scholar 

  • Darvey, N.L.; Gustafson, J.P. (1975): Identification of rye chromosomes in wheat-rye addition lines and triticale by heterochromatin bands. Crop Sci. 15, 239–243

    Google Scholar 

  • Giraldez, R.; Cermeno, M.C.; Orellana, J. (1979): Comparison of C-banding pattern in the chromosomes of inbred lines and open varieties of rye (Secale cereale L.). Z. Pflanzenzücht. 83, 40–48

    Google Scholar 

  • Gustafson, J.P. (1976): The evolutionary development of triticale: the wheat-rye hybrid. Evol. Biol. 9, 107–135

    Google Scholar 

  • Gustafson, J.P. (1982): Cytogenetics of Triticale. In: Cytogenetics of Crop Plants (eds. Swaminathan, M.S.; Gupta, R.K.; Sinha, N.) London: Macmillan (in press)

    Google Scholar 

  • Gustafson, J.P.; Bennett, M.D. (1976): Preferential selection for wheat-rye substitutions in 42-chromosome triticale. Crop Sci. 16, 688–693

    Google Scholar 

  • Gustafson, J.P.; Bennett, M.D. (1982): The effect of telomeric heterochromatin from Secale cereale L. on triticale (X Triticosecale Wittmack). I. The influence of several blocks of telomeric heterochromatin on early endosperm development and kernel characteristics at maturity. Can. J. Genet. Cytol. (in press)

  • Hennen, W.K.; Casperson, T. (1973): Identification of the chromosomes of rye by distribution patterns of DNA. Hereditas 74, 259–272

    Google Scholar 

  • Jones, J. (1980): Ph. D. thesis. University of Cambridge, England

  • Jones, R.N.; Rees, H. (1968): The influence of B-chromosomes upon the nuclear phenotype of rye. Chromosoma 24, 158–176

    Google Scholar 

  • Kranz, A.R.; Goethe, J.W. (1976): Karyotype analysis in meiosis; giemsa banding in the genus Secale L. Theor. Appl. Genet. 46, 101–107

    Google Scholar 

  • Lima-de-Faria, A. (1952): Pachytene analysis of the chromosome complement of rye. Chromosoma 5, 1–68

    Google Scholar 

  • Lukaszewski, A.J.; Apolinarska, B.; Lapinski, B.; Sodkiewicz, W. (1980): Chromosome elimination in the Triticum persicum X T. dicoccoides) X Secale cereale F1 hybrids. Hod. Rosl. Aklim. Nasien. 24, 365–372

    Google Scholar 

  • Merker, A. (1976): The cytogenetic effect of heterochromatin in hexaploid triticale. Hereditas 83, 215–222

    Google Scholar 

  • Nishikawa, K. (1970): DNA content of the individual chromosomes and genomes in wheat and its relatives. Seiken Ziho 22, 57–65

    Google Scholar 

  • Pieritz, W.J. (1970): Elimination von Chromosomen in Amphidiploiden Weizen-Roggen-Bastarden (Triticale). Z. Pflanzenzücht. 64, 90–109

    Google Scholar 

  • Radley, J.M. (1966): DNA content of individual chromosomes of Protomnodon bicolor. Exp. Cell Res. 41, 217–220

    Google Scholar 

  • Rees, H.; Hutchinson, J. (1973): Nuclear DNA variation due to B chromosomes. Cold Spring Harbor Symp. Quant. Biol. 38, 175–181

    Google Scholar 

  • Roupakias, D.G.; Kaltsikes, P.J. (1977): The effect of telomeric heterochromatin on chromosome pairing of hexaploid triticale. Can. J. Genet. Cytol. 19, 543–548

    Google Scholar 

  • Thomas, H.M. (1981): The giemsa C-band karyolypes of six Lolium species. Heredity 46, 263–267

    Google Scholar 

  • Verma, S.C.; Recs, H. (1974): Giemsa staining and the distribution of heterochromatin in rye chromosomes. Heredity 32, 118–122

    Google Scholar 

  • Wcimarck, A. (1975): Heterochromatin polymorphism in rye karyotypes as detected by the giemsa C-banding technique. Hereditas 79, 293–300

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Mcehclkc

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lukaszewski, A.J., Wozna, J., Apolinarska, B. et al. The DNA content of the individual chromosomes of rye. Theoret. Appl. Genetics 62, 145–148 (1982). https://doi.org/10.1007/BF00293347

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00293347

Key words

Navigation