Abstract
In order to investigate wheter colchicine affects crossing-over, rye anthers of an inbred line of rye forming bridges and fragments at anaphase I produced by erroneous chiasmata, and anthers of plants heterozygous for a conspicuous heterochromatin band, were cultured in a medium with colchicine. Anthers planted at zygotene did not show bridges at AI in the inbred line. In the heterozygotes no difference between associated chromatids in respect to the heterochromatin band, resulting from crossingover, were observed. In anthers planted at pachytene both bridges and chromosomes showing difference between associated chromatids were observed at a stage equivalent to AI with the same frequency as in anaphase I cells of untreated anthers. This demonstrates that crossing-over or a prerequisite to crossing-over is established at zygotene, and also that absence of chiasmate association at later stages is not due to precocious slipping off of chiasmata.
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de la Peña, A., Puertas, M.J., Cermeño, M.C. et al. Evidence of crossing-over inhibition in rye anthers cultured with colchicine. Chromosoma 72, 151–155 (1979). https://doi.org/10.1007/BF00293231
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DOI: https://doi.org/10.1007/BF00293231