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Synaptonemal complex spreading in Allium cepa and A. fistulosum

I. The initiation and sequence of pairing

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Abstract

The general features and fine structure of homologous chromosome alignment and pairing have been investigated in two species of Allium (A. fistulosum and A. cepa), which have similar karyotypes but very different patterns of chiasma distribution. Although there is no support for the occurrence of a general pre-meiotic alignment of homologous chromosomes, both species show some alignment of homologues as an immediate prelude to synaptonemal complex (SC) formation. In both species pairing usually commences at sub-terminal sites and is succeeded by numerous separate intercalary initiations of pairing in interstitial and distal regions and then in proximal regions. The last parts to pair, in both species, are pericentromeric and telomeric regions. There is, therefore, no evident relationship between the sequence of pairing and chiasma distribution in these species. Regularly alternating convergences and divergences of aligned axial cores (ACs), termed multiple association sites, are frequently observed. It is proposed that these represent potential pairing initiation sites and from observations on their spatial distribution it is argued that they may be evenly distributed through most of the genome. Small spherical or ellipsoid nodules are found at association sites and between closely aligned ACs which persist in the SC segments present during zygotene, but most of them disappear abruptly at the end of zygotene. These are termed zygotene nodules (ZN) and it is proposed that they are involved in matching corresponding sites on homologous chromosomes as well as possibly having a recombinational role. Their composition, structure, mode of action and relationship to pachytene recombination nodules are at present unknown.

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  1. Department of Genetics, The University of Birmingham, B15 2TT, Birmingham, UK

    S. M. Albini & G. H. Jones

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  1. S. M. Albini
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  2. G. H. Jones
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Albini, S.M., Jones, G.H. Synaptonemal complex spreading in Allium cepa and A. fistulosum . Chromosoma 95, 324–338 (1987). https://doi.org/10.1007/BF00293179

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  • DOI: https://doi.org/10.1007/BF00293179

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