Abstract
The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques. —Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern. Conversely, replacement of BrdU containing medium by another one with thymidine yields G-type replication patterns. A thymidine pulse during the first 4 h of S-phase results in R-type replication patterns; from 7–10 h after block removal it produces G-type pattern. In between, only faint red staining dots can be found indicating a marked decrease of replicational activity during the middle part of the S-phase.
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Calderon,D., Schnedl, W.: A comparison between quinacrine fluorescence banding and 3H-thymidine incorporation. Humangenetik 18, 63–70 (1973)
Couturier,J., Dutrillaux,B., Lejeune,J.: Etude des fluorescences spécifique des bandes R et des bandes Q des chromosomes humains. C.R. Acad. Sci. (Paris) 276, 339–342 (1973)
Dutrillaux, B.: Traitements discontinus par le BrdU et coloration par l'acridine orange: obtention de marquages R, Q et intermédiaires. Chromosoma (Berl.) 52, 261–273 (1975)
Dutrillaux, B., Couturier, J., Richer, C.-L., Viegas-Péquignot, E.: Sequence of DNA replication in 277 R- and Q-bands of human chromosomes using a BrdU treatment. Chromosoma (Berl.)58,51–61 (1976)
Epplen, J.T., Siebers, J.-W., Vogel, W.: DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique. Cytogenet. Cell Genet. 15, 177–185 (1975)
Ganner, E., Evans, H.J.: The relationship between pattern of DNA replication and of quinacrine fluorescence in the human chromosome complement. Chromosoma (Berl.) 35, 326–341 (1971)
Grzeschik, K.-H., Kim, M.A., Johannsmann, R.: Late replicating bands of human chromosomes demonstrated by fluorochrome and Giemsa staining. Humangenetik 29, 41–59 (1975)
Hamilton, A.J.: Cell population kinetics: A modified interpretation of the graph of labeled mitoses. Science 164, 952–954 (1969)
Kikuchi, Y., Sandberg, A.A.: Chronology and pattern of human chromosome replication. I. Blood leukocytes of normal subjects. J. nat. Cancer Inst. 32, 1109–1144 (1964)
Kim, M.A., Johannsmann, R., Grzeschik, K.-H.: Giemsa staining of the sites replicating DNA early in human lymphocyte chromosomes. Cytogenet. Cell Genet. 15, 363–371 (1975)
Latt,S.A.: Optical studies of metaphase chromosome organization. Ann. Rev. Biophys. Bioeng. 5, 1–37 (1976)
Remington,J.A., Klevecz, R.R.: Families of replicating units in cultured hamster fibroblasts. Exp. Cell Res. 76, 410–418 (1973)
Schmid, W.: DNA replication patterns of human chromosomes. Cytogenetics 2, 175–193 (1963)
Stubblefield,E.,Gay,M.: Quantitative tritium autoradiography of mammalian chromosomes. II. The kinetics of DNA synthesis in individual chromosomes of Chinese hamster fibroblasts. Chromosoma (Berl.) 31, 79–90 (1970)
Wolf, U.: Cell cultures from tissue explants: Culture and preparation of cells from amniotic fluid. In: Methods in human cytogenetics (H.G. Schwarzacher and U. Wolf, eds.). Berlin-Heidelberg-New York: Springer 1974
Zeuthen, E.: Thymine starvation by inhibition of uptake and synthesis of thymine compounds in Tetrahymena. Exp. Cell Res. 50, 37–46 (1968)
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Schempp, W., Vogel, W. Decrease of DNA synthesis in amniotic fluid cells during the middle part of S-phase revealed by differential chromosome staining after incorporation of BrdU. Chromosoma 67, 193–199 (1978). https://doi.org/10.1007/BF00293176
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DOI: https://doi.org/10.1007/BF00293176