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Chromosomal localization of ribosomal 5 S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA

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Abstract

5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 107 dpm/μg with carrier-free iodine-125. After hybridization in situ of 125I-5S RNA and autoradiography only region B3c-e (containing two “normal” and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly “heterozygous” labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorporation in spite of a continuous synthesis of 5 S RNA in salivary gland cells.

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  1. Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, DDR-4325, Gatersleben, German Democratic Republic

    Helmut Bäumlein & Ulrich Wobus

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  1. Helmut Bäumlein
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  2. Ulrich Wobus
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Bäumlein, H., Wobus, U. Chromosomal localization of ribosomal 5 S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA. Chromosoma 57, 199–204 (1976). https://doi.org/10.1007/BF00292918

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  • DOI: https://doi.org/10.1007/BF00292918

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