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Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes

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Abstract

Human metaphase chromosomes were stained with silver following a pretreatment with a heated alkaline solution. The most conspicuous feature of the stained metaphases was the omission of silver staining in the secondary constrictions of chromosomes 1, 9 and 16, and on the distal Yq. Our evidence indicates that the negative silver binding is due to the preferential removal or alteration of non-histone proteins associated with these regions. The cytochemical significance of these findings is discussed.

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Authors and Affiliations

  1. Department of Biology, Samford University, 35209, Birmingham, Alabama, USA

    William Mike Howell & Thomas E. Denton

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  1. William Mike Howell
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  2. Thomas E. Denton
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Howell, W.M., Denton, T.E. Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes. Chromosoma 57, 165–169 (1976). https://doi.org/10.1007/BF00292914

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  • DOI: https://doi.org/10.1007/BF00292914

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