Abstract
The course of DNA synthesis in the chromosomes was studied in synchronized human lymphocyte cultures, by means of the BrdU-Hoechst-Giemsa method. In comparing replication patterns and G-banding it was found that with regard to banding the process of DNA replication can be divided into two separate phases, an “early replication period” which is characterized by DNA synthesis in R bands of the autosomes and active X chromosome, and a “late replication period” which concerns the G-positive regions of the autosomes and all the bands of the heterochromatic X and Y chromosomes. No overlapping was found between the two phases mentioned. The possible role of regulatory mechanisms was discussed.
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Bianchi, N.O., Bianchi, M.S.A. de: DNA replication sequence of human chromosomes in blood cultures. Chromosoma (Berl.) 17, 273–280 (1965)
Camevali, F., Mariotti, D.: Variations of the length of S phase related to the time cells are blocked at the G1S interphase. Chromosoma (Berl.) 63, 33–37, 1977
Collins, J.M.: Rates of DNA synthesis during the S phase of HeLa cells. J. biol. Chem. 253, 8570–8577 (1978)
Dutrillaux, B., Couturier, J., Richer, C.L., Viegas-Pequignat, E.: Sequence of DNA replication in 277 R and Q bands of human chromosomes using a BUdR treatment. Chromosoma (Berl.) 58, 51–61 (1976)
Epplen, J.T., Siebers, J.W., Vogel, W.: DNA replication pattern of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique. Cytogenet. Cell Genet. 15, 177–191 (1975)
Farber, R.A., Davidson, R.L.: Altered pattern of replication of human chromosomes in a human fibroblasts — mouse cell hybrid. Proc. nat. Acad. Sci. (Wash.) 75, 1470–1474 (1978)
Grzeschik, K.H., Kim, M.A., Johannsmann, R.: Late replicating bands of human chromosomes demonstrated by fluorochrome and Giemsa staining. Humangenetic 29, 41–59 (1975)
Kim, M.A., Johannsmann, R., Grzeschik, K.H.,: Giemsa staining of the sites replicating DNA early in human lymphocyte chromosomes. Cytogenet. Cell Cenet. 15, 363–372 (1975)
Latt, S.A.: Fluorescence analysis of late DNA replication in human metaphase chromosomes. Somatic Cell Genet. 1, 293–302 (1975)
Rueckert, R.R., Mueller, G.C.: Studies on unbalanced growth in tissue cultures I. Induction and consequences of thymidine defficiency. Cancer Res. 20, 1584–1591 (1960)
Schempp, W., Vogel, W.: Decrease of DNA synthesis in amniotic fluid cells during the middle part of S phase, revealed by differential chromosome staining after incorporation of BrdU. Chromosoma (Berl.) 67, 193–199 (1978)
Schwarzacher, H.G., Wolf, U.: Methods in human cytogenetics. pp. 110–111, 114. Berlin, Heidelberg, New York: Springer 1974
Steffen, J.A., Stolzmann, W.M.: Studies on in vitro lymphocyte proliferation in cultures synchronized by the inhibition of DNA synthesis. Exp. Cell Res. 56, 453–460 (1969)
Stubblefield, E.: Analysis of the replication pattern of Chinese hamster chromosomes using 5-bromodeoxyuridine suppression of 33258 Hoechst fluorescence. Chromosoma (Berl.) 53, 209–221 (1975)
Taylor, J.H.: Increase in DNA replication sites in cells held at the beginning of the S phase. Chromosoma (Berl.) 62, 291–300 (1977)
Volpe, P., Eremenko, T.: A language of DNA modification during its replication. Proc. of the XIV International Congress of Genetics, part I, 194. Moscow 1978
Zakharov, A.F., Egolina, N.A.: Correlations between patterns of DNA replication and chromosome banding. Biol. Zbl. 95, 327–334 (1976)
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Schmidt, M. Two phases of DNA replication in human cells. Chromosoma 76, 101–110 (1980). https://doi.org/10.1007/BF00292230
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DOI: https://doi.org/10.1007/BF00292230