Abstract
By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5′-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
This is a preview of subscription content, log in via an institution to check access.
Access this article
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
Similar content being viewed by others
References
Allen, R. (1974). Polyacrylamide gel electrophoresis with discontinuous buffers at a constant pH. In Electrophoresis and Isoelectric Focusing in Polyacrylamide Gel, R. Allen, H. Maurer, eds. (Berlin: Walter de Gruyter), pp. 105–114.
Buchberg, A.M., Camper, S.A. (1993). Mouse Chromosome 11. Mamm. Genome 4 (Suppl.), S164-S175.
Davidson, R., Gerald, P. (1976). Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somat. Cell Genet. 2, 165–176.
Echard, G., Yerle, M., Millan, D., Archibald, A.L. (1993). The gene map of the pig (Sus scrofa domestica I.). In Genetic Maps. Locus Maps of Complex Genomes, 6th ed. S.J. O'Brien, ed. pp. 4.241–4.249.
Gradov, A., Pack, S.D., Sukoyan, M., Rubtsov, N., Bochkarev, M., Serov, O. (1985). Regional assignment of the genes for TK1, GALK, ALDC, and ESD on chromosome 8 in the American mink by chromosome-mediated transfer. Mol. Gen. Genet. 200, 433–438.
Kaftanovskaya, H.M., Serov, O.L. (1992). High-resolution G-banding of sheep chromosomes. J. Hered. 83, 92–99.
Leong, M.M.L., Lin, C.C., Ruth, R.F. (1983). Assignment of superoxide dismutase (SOD-1) gene to chromosome no. 9 of domestic pig. Can. J. Genet. Cytol. 25, 233–238.
Mandahl N., Fredga, K. (1975). Q, G and C-band patterns of the mink chromosomes. Hereditas 81, 211–220.
Matyakhina, L.D., Zhdanova, N.S., Matveeva, N.M., Pack, S.D., Serov, O.L. (1992). Monosomic hybrid clones containing particular human 15, 21 and X chromosomes. Genetika (Russian) 27, 125–131.
O'Brien, S.L., Peters, J., Searle, A., Womack, J., Graves, J.A. (1993). Report of the Committee on Comparative Gene Mapping. Genome Prior. Rep. 1, 758–809.
Pack, S.D., Zhdanova, N.S., Sukoyan, M.A., Serov, O.L. (1989). Chromosomal and regional localization of the genes for UMPH2, APRT, PEPD, PEPS, PSP, and PGP in mink: comparison with man and mouse. Cytogenet. Cell Genet. 50, 127–131.
Rubtsov, N.B., Gradov, A.A., Serov, O.L. (1982). Chromosome localization of the loci GOT1, PP, NP, SOD1 in the American mink (Mustela vison). Theor. Appl. Genet. 63, 331–336.
Ryttman, H., Trebo, P., Gustavsson, I., Gahne, R., Tuncja, R. (1986). Further data on chromosomal assignments of pig enzyme loci LDHA, LDHB, MPI, PEPB, and PGM1, using somatic cell hybrids. Anim. Genet. 17, 323–333.
Ryttman, H., Trebo, P., Gustavsson, I. (1988). Regional assignment of NP and MPI on chromosome 7 in pig, Sus scrofa. Anim. Genet. 19, 197–200.
Standard karyotype of domestic pig. Committee for Standardized Karyotype of the Domestic pig. (1988). Heredity 109, 151–157.
Thomsen, P.D., Bosma, A.A., Kaufman, U., Harbitz, I. (1991). The porcine PGD gene is preferentially lost from chromosome 6 in pigrodent somatic cell hybrids. Hereditas 115, 63–67.
Wilson, D., Smallow, D., Povey, S. (1986). Assignment of the human gene for uridine 5′-monophosphate phosphohydrolase (UMPH2) to the long arm of chromosome 17. Ann. Hum. Genet. 50, 223–227.
Yerle, M., Galman, O., Echard, G. (1991). The high-resolution GTG-banding pattern of pig chromosomes. Cytogenet. Cell Genet. 56, 45–47.
Zhdanova, N.S., Urzhenko, A.V., Gradov, A.A. (1984). Obtaining and chromosomal analysis of the American mink (Mustela vision) clonal culture cells resistant to 5-bromodeoxyuridine. Tsitologia (Russian) 25, 177–181.
Zijlstra, C., Bosma, A.A., de Haan, N.A., Thomsen, P.D., Tucker, E.M. (1992). Characterization of pig-rodent somatic cell hybrids. Proc. 10th Eur. Colloquium on Cytogenetics of Domestic Animal. Utrecht Univ., The Netherlands, August 18–21, 1992, pp. 290–294.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhdanova, N.S., Astakhova, N.M., Kuznetsov, S.B. et al. Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12. Mammalian Genome 5, 781–784 (1994). https://doi.org/10.1007/BF00292013
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00292013