Abstract
We present here the fine genetic mapping of the proximal part of mouse Chromosome (Chr) 12 between D12Mit54 and D12Mit4. This chromosomal region contains three loci, Pax9, Tcf3a, and Acrodysplasia (Adp), which seem to play an important role in pattern formation during mouse embryogenesis. The Adp mutation, which was created by transgene integration, causes skull, paw, and tail deformities. Pax9, which is expressed in the face, paws, and tail, once qualified as a possible candidate for the Adp locus. We analyzed 997 interspecific backcross progeny for recombination between the markers D12Mit54 and D12Mit4; we recovered 117 recombinants, which were further typed for Pax9, Tcf3a, Adp, D12Mit88, D12Nds1, D12Mit36, and D12Mit34. This study represents the first instance in which all the above loci have been included in a single analysis, thereby allowing unambiguous determination of the genetic order and distance between D12Mit54 and D12Mit4. From our results, we conclude that the Adp locus is distinct from either Pax9 or Tcf3a.
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Avraham, K.B., Prezioso, V.R., Chen, W.S., Lai, E., Sladek, F.M., Zhong, W., Darnell, J.E., Jr., Jenkins, N.A., Copeland, N.G. (1992). Murine chromosomal location of four hepatocyte-enriched transcription factors: HNF-3α, HNF-3β, HNF-3γ, and HNF-4. Genomics 13, 264–268.
Balling, R., Deutsch, U., Gruss, P. (1988). Undulated, a mutation affecting the development of the mouse skeletop, has a point mutation in the paired box of Pax1. Cell 55, 531–535.
Church, G.M., Gilbert, W. (1984). Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991–1995.
DeLoia, J.A., Solter, D. (1990). A transgene insertional mutation at an imprinted locus in the mouse genome. Development, Supplement, 73–79.
Dietrich, W., Katz, H., Lincoln, S.E., Shin, H-S, Friedman, J., Dracopoli, N.C., Lander, E.S. (1992). A genetic map of the mouse suitable for typing interspecific crosses. Genetics 131, 423–447.
Epstein, D.J., Vekemans, M., Gros, P. (1991). Splotch (Sp2h), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3. Cell 67, 767–774.
Hill, R.E., Favor, J., Hogan, B.L.M., Ton, C.C.T., Saunders, G.F., Hanson, I.M., Prosser, J., Jordan, T., Hastie, N.D., van Heyningen, V. (1991). Mouse small eye results from mutation in a paired-like homeobox-containing gene. Nature 354, 522–525.
Lai, E., Prezioso, V.R., Smith, E., Litvin, O., Costa, R.H., Darnell, J.E., Jr., (1990). HNF-3A, a hepatocyte-enriched transcription factor of novel structure is regulated transcriptionally. Genes Dev 4, 1427–1436.
Lai, E., Prezioso, V.R., Tao, W., Chen, W.S., Darnell, J.E., Jr. (1991). Hepatocyte nuclear factor 3⋏ belongs to a gene family in mammals that is homologus to the Drosophila homeotic gene fork head. Genes Dev. 5, 416–427.
Law, M.F., Byrne, J.C., Howley, P.M. (1983). A stable bovine papilloma virus hybrid plasmid that expresses a dominant selective trait. Mol. Cell. Biol. 3, 2110–2115.
Meisler, M.H., (1992). Insertional mutation of ‘classical’ and novel genes in transgenic mice. Trends Genet. 8, 341–344.
Monaghan, A.P., Kaestner, K.H., Grau, E., Schütz, G. (1993). Postimplantation expression patterns indicate a role for the mouse forkhead/HNF-3α, β and γ genes in determination of the definitive endoderm, chordamesoderm and neuroectoderm. Development 199, 567–578.
Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).
Sasaki, H., Hogan, B.L.M. (1993). Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Development 118, 47–59.
Sasaki, H., Hogan, B.L.M. (1994). HNF-3β as a regulator of floor plate development. Cell 76, 103–115.
Wallin, J., Mizutani, Y., Imai, K., Miyashita, N., Moriwaki, K., Taniguchi, M., Koseki, H., Balling, R. (1993). A new Pax gene, Pax-9, maps to Chromsome 12. Mamm. Genome 4, 354–358.
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Watanabe, T., Tarttelin, E., Neubüser, A. et al. Fine genetic mapping defines the genetic order of Pax9, Tcf3a, and Acrodysplasia (Adp). Mammalian Genome 5, 768–770 (1994). https://doi.org/10.1007/BF00292010
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DOI: https://doi.org/10.1007/BF00292010