Summary
Viral leukemogenesis in mice is frequently initiated by proviral activation of a highly conserved cellular gene called Pim-1. Here we report the chromosomal localization of the human homologue by Southern blot analyses of DNAs obtained from human-rodent somatic cell hybrids. The single copy human homologue was assigned to the 6pter-q12 segment.
Similar content being viewed by others
References
Bishop JM, Varmus HE (1982) RNA tumor viruses. In: Weiss RA, Teich N, Varmus HE, Coffin J (eds) The molecular biology of tumor viruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 999–1009
Bodmer WF, Bodmer JG (1978) Evolution and function of the HLA system. Br Med Bull 34:309–316
Corcoran LM, Adams JM, Dunn AR, Cory S (1984) Murine T lymphomas in which the cellular myc oncogene has been activated by retroviral insertion. Cell 37:113–122
Croce CM, Thierfelder W, Erikson J, Nishikura K, Finan J, Lenoir GM, Nowell P (1983) Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a Cλ locus in Burkitt lymphoma cells. Proc Natl Acad Sci USA 80:6922–6926
Cuypers HT, Selten G, Quint W, Zijlsta M, Robanus-Maandag E, Boelens W, van Wezenbeek P, Melief C, Berns A (1984) Murine leukemia virus-induced T-cell lymphomagenesis: integration of proviruses in a distinct chromosomal region. Cell 37:141–150
Fung YKT, Lewis WG, Kung HJ, Crittenden LB (1983) Activation of the cellular oncogene c-erbB by LTR insertion; molecular basis for induction of erythroblastosis by avian leukosis virus. Cell 33:357–368
Geurts van Kessel AHM, ten Brinke H, Boere WAM, den Boer WC, de Groot PG, Hagemeijer A, Meera Khan P, Pearson PL (1981) Characterization of the Philadelphia chromosome by gene mapping. Cytogenet Cell Genet 30:83–91
Geurts van Kessel AHM, Tetteroo PAT, von dem Borne AEGKr, Hagemeijer A, Bootsma D (1983) Expression of human myeloid associated surface antigens in human-mouse myeloid cell hybrids. Proc Natl Acad Sci USA 80:3748–3751
Hayward WS, Neel BG, Astrin S (1981) Activation of a cellular onc gene by promotor insertion in ALV-induced lymphoid leukosis. Nature 290:475–480
Heisterkamp N, Stephenson JR, Groffen J, Hansen PF, de Klein A, Bartram CR, Grosveld G (1983) Localization of the c-able oncogene adjacent to a translocation breakpoint in chronic myelocytic leukemia. Nature 306:239–242
Hilkens J, Cuypers HTh, Selten G, Kroezen V, Hilgers J, Berns A (1986) Genetic mapping of the Pim-1 putative oncogene to mouse chromosome 17. Somatic Cell Mol Genet 12:1–8
Klein G (1983) Specific chromosomal translocations and the genesis of B-cell-derived tumors in mice and man. Cell 32:311–315
Leder P, Battey J, Lenoir G, Moulding C, Murphy W, Potter H, Stewart T, Taub R (1983) Translocation among antibody genes in human cancer. Science 222:765–771
Miyamoto K, Tomita N, Ishii A, Nonaka H, Kondo T, Tanaka T, Kitajima K (1984) Chromosome abnormalities of leukemia cells in adult patients with T-cell leukemia. JNCI 73:353–362
Nadeau JH, Taylor B (1984) Lengths of chromosomal segments conserved since divergence of man and mouse. Proc Natl Acad Sci USA 81:814–818
Neel BG, Hayward WS, Robinson HL, Fang J, Astrin SM (1981) Avian leukosis virus-induced tumors have common proviral integration sites and synthesize discrete new RNAs: oncogenesis by promotor insertion. Cell 23:323–334
Neil JC, Hughes D, McFarlane R, Wilkie NM, Onions DE, Lees G, Jarret O (1984) Transduction and rearrangement of the myc gene by feline leukaemia virus in naturally occurring T-cell leukemias. Nature 308:814–820
Nusse R, Varmus HE (1982) Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell 31:99–109
Payne GS, Bishop JM, Varmus HE (1982) Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas. Nature 295:209–214
Peters G, Brookes S, Smith R, Dickson C (1983) Tumorigenesis by mouse mammary tumor virus: evidence for a common region for provirus integration in mammary tumors. Cell 33:369–377
Robson EB, Lamm LU (1983) Report of the committee on the genetic constitution of chromosome 6. Cytogenet Cell Genet 37:47–70
Selten G, Cuypers HT, Zijlstra M, Melief C, Berns A (1984) Involvement of c-myc in MuLV-induced T cell lymphomas in mice: frequency and mechanisms of activation. EMBO J 3:3215–3222
Selten G, Cuypers HTh, Berns A (1985) Proviral activation of the putative oncogene Pim-1 in MuLV induced T-cell lymphomas. EMBO J 4:1793–1798
Swift RA, Shaller E, Witter RL, Kung HJ (1985) Insertion activation of c-myc by reticuloendotheliosis virus in chicken B lymphoma: nonrandom distribution and orientation of the proviruses. J Virol 54:869–872
Teich N, Wyke J, Mak T, Bernstein A, Hardy W (1982) Pathogenesis of retrovirus-induced disease. In: Weiss RA, Teich N, Varmus HE, Coffin J (eds) RNA tumor viruses. The molecular biology of tumor viruses. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 785–998
van Someren H, Westerveld A, Hagemeijer A, Mees JR, Meera Khan P, Zaalberg OB (1974) Human antigen and enzyme markers in man-Chinese hamster somatic cell hybrids: evidence for synteny between HL-A, PGMe, ME1 and IPO-B loci. Proc Natl Acad Sci USA 71:962–965
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cuypers, H.T., Selten, G., Berns, A. et al. Assignment of the human homologue of Pim-1, a mouse gene implicated in leukemogenesis, to the pter-q12 region of chromosome 6. Hum Genet 72, 262–265 (1986). https://doi.org/10.1007/BF00291892
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00291892