Summary
Variants in structure and content of the human Y chromosome were investigated by cytogenetic methods and by restriction endonuclease analysis. It is shown that most or all of the 2.1 kb male-specific reiterated sequence is located in the distal part of the brightly fluorescing heterochromatin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bostock CJ, Gosden JR, Mitchell AR (1978) Localisation of a male-specific DNA fragment to a sub-region of the human Y-chromosome. Nature 272:324–328
Caspersson T (1973) Procedure for the study of the reproducibility of normal mammalian banding patterns and for analysis of aberrant chromosome patterns. In: Caspersson T, Zech L (eds) Chromosome identification: Techniques and applications in biology and medicine. Nobel Symposium 23: 50–55
Caspersson T, Zech L, Johannsson C, Modest EJ (1970) Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma 30:215–227
Cooke H (1976) Repeated sequence specific to human males. Nature 262:182–186
Cooke HJ, Noel B (1979) Confirmation of Y/autosome translocations using recombinant DNA. Hum Genet 50:39–44
Jalal SM, Pfeiffer RA, Pathak S, Hsu TC (1974) Subdivision of the human Y chromosome. Hum Genet 24:59–65
Kim MA, Bier L, Pawlowitzki IH, Pfeiffer RA (1971) Human Y chromosome with two fluorescing bands after staining with quinacrine derivatives. Hum Genet 13:238–240
Kinross, J, Fraccaro M, Scappaticci, S, Tiepolo L, Zuffardi O, Pawlowitzki IA, Jones KW (1978) BSu restriction of DNA from cases exhibiting sex-chromosome abnormalities. Cytogenet Cell Genet 20:59–69
Kirby KS (1968) Isolation of nucleic acids with phenolic solvents. In: Grossman L, Moldave K (eds) Methods in Enzymology Vol 12 B. Academic Press, New York, pp 87–112
McKay RDG, Heritage J, Bobrow M, Cooke HJ (1978) Endonuclease analysis of Y chromosome DNA. Cytogenet Cell Genet 22:357–358
Pfeiffer RA (1974) Cell cultures from blood and bone marrow. In: Schwarzacher HG, Wolf U (eds) Methods in human cytogenetics. Springer, Berlin Heidelberg New York, pp 1–37
Schmid M (1979) Demonstration of Y/autosomal translocations using distamycin A. Hum Genet 53:107–109
Schwarzacher HG (1974) Preparation of metaphase chromosomes. In: Schwarzacher HG, Wolf U (eds) Methods in human cytogenetics. Springer, Berlin Heidelberg New York, pp 71–81
Schweizer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes by prestaining with a DNA binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332
Szabo P, Kunkel L, Yu LC, George D, Smith KK (1980) Chromosomal distribution of DNA sequences derived from the human Y chromosome in human higher primates. Cytogenet Cell Genet 25:212–213
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schmidtke, J., Schmid, M. Regional assignment of a 2.1-kb repetitive sequence to the distal part of the human Y heterochromatin. Hum Genet 55, 255–257 (1980). https://doi.org/10.1007/BF00291774
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00291774