Skip to main content
SpringerLink
Log in

Independent loss of human hexosaminidases A and B in man-Chinese hamster somatic cell hybrids

  • Original Investigations
  • Published:
Humangenetik Aims and scope Submit manuscript

Summary

The presence or absence of human hexosaminidases A and B was studied in 105 man-Chinese hamster hybrid cell clones. The results show that these two isoenzymes behave as independent markers.

Zusammenfassung

Das Vorkommen oder Fehlen der menschlichen Hexosaminidasen A und B wurde in 105 Zellklonen aus Zellhybriden Mensch/Chinesischer Hamster untersucht. Die Ergebnisse zeigen, daß die beiden Isoenzyme sich als voneinander unabhängige genetische Marker verhalten.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Desnick, R. J., Snyder, P. D., Desnick, S. J., Krivit, W., Sharp, H. L.: Sandhoff's disease: Ultrastructural and biochemical studies. In: Sphingolipids, sphingolipidoses and allied disorders, B.W. Volk and S. M. Aronson (Eds.), pp. 351–371. New York-London: Plenum Press 1972.

    Google Scholar 

  • Hooghwinkel, G. J. M., Veltkamp, W. A., Overdijk, B., Lisman, J. W.: Electrophoretic separation of β-N-acetylhexosaminidases of human and bovine brain and liver and of Tay-Sachs brain tissue. Z. physiol. Chem. 353, 839–841 (1972).

    Google Scholar 

  • Meera Khan, P.: Enzyme electrophoresis on cellulose acetate gel: Zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybrids. Arch. Biochem. Biophys. 145, 470–483 (1971).

    Google Scholar 

  • O'Brien, J. S., Okada, S., Ho, M. W., Fillerup, D. L., Veath, M. L., Adams, K. Ganglioside storage diseases. Fed. Proc. 30, 956–969 (1971).

    Google Scholar 

  • Okada, S., O'Brien, J. S.: Tay-Sachs disease: Generalized absence of a beta-D-N-acetyl-hexosaminidase component. Science 165, 698–700 (1969).

    Google Scholar 

  • Okada, S., Veath, M. L., O'Brien, J. S.: Juvenile GM2 gangliosidosis: Partial deficiency of hexosaminidase A. J. Pediat. 77, 1063–1065 (1970).

    Google Scholar 

  • Robinson, D., Stirling, J. L.: N-acetyl-β-glucosaminidases in human spleen. Biochem. J. 107, 321–327 (1968).

    Google Scholar 

  • Sandhoff, K., Andreae, U., Jatzkewitz, H.: Deficient hexosaminidase activity in an exceptional case of Tay-Sachs disease with additional storage of kidney globoside in visceral organs. Life Sci. 7, 283–288 (1968).

    Google Scholar 

  • Sandhoff, K., Harzer, K., Wässle, W., Jatzkewitz, H.: Enzyme alterations and lipid storage in three variants of Tay-Sachs disease. J. Neurochem. 18, 2469–2489 (1971).

    Google Scholar 

  • Snyder, P. D., Jr., Krivit, W., Sweeley, C. C.: Generalized accumulation of neutral glycosphingolipids with GM2 ganglioside accumulation in the brain. J. Lipid Res. 13, 128–136 (1972).

    Google Scholar 

  • Tateson, R., Bain, A. D.: GM2 gangliosidosis: Consideration of the genetic defects. Lancet 1971 II, 612–613.

    Google Scholar 

  • Westerveld, A., Visser, R. P. L. S., Meera Khan, P., Bootsma, D.: Loss of human genetic markers in man-Chinese hamster somatic cell hybrids. Nature (Lond.) New Biol. 234, 20–24 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medical Biological Laboratory TNO, 139 Lange Kleiweg, Rijswijk (ZH), The Netherlands

    Hans Beijersbergen van Henegouwen

  2. Laboratory for Molecular Genetics, State University Leiden, c/o P.O. Box 45, 2100, Rijswijk, The Netherlands

    Harry van Someren

Authors
  1. Harry van Someren
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hans Beijersbergen van Henegouwen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported in part by the Netherlands Foundation for Medical Research.

Rights and permissions

Reprints and permissions

About this article

Cite this article

van Someren, H., van Henegouwen, H.B. Independent loss of human hexosaminidases A and B in man-Chinese hamster somatic cell hybrids. Humangenetik 18, 171–174 (1973). https://doi.org/10.1007/BF00291485

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00291485

Keywords

Search

Navigation