Summary
We have demonstrated close linkage between the genes for apolipoprotein E (apoE) and apolipoprotein CII (apoCII). Families segregating for apoE protein variants were screened for a DNA restriction fragment length polymorphism close to the apoCII gene by using an apoCII cDNA clone. The maximum lod score is 4.52 (sexes combined) at a recombination frequency of zero. Given linkage, it may be assumed that no recombinations have happened in altogether 33 observed meioses. It is therefore evident that the apoCII gene is situated on chromosome 19, close to the apoE gene.
Similar content being viewed by others
References
Breckenridge WC, Little JA, Steiner G, Chow A, Poapst M (1978) Hypertriglyceridaemia associated with deficiency of apolipoprotein CII. N Engl J Med 298:1265–1272
Breslow JL, McPherson J, Karathanasis SK (1983) ApoE gene contains tandemly repeated 66 bp DNA sequences highly homologous to similar repeated sequences in the apoAI gene. Circulation 68: 16 (Suppl. III)
Cox DW, Breckenridge WC, Little JA (1978) Inheritance of apolipoprotein CII deficiency with hypertriglyceridaemia and pancreatitis. N Engl J Med 299:1421–1424
Gerald PS, Grzeschik KH (1984) Report of the committee on the genetic constitution of chromosomes 10, 11 and 12. Cytogenet Cell Genet 37:103–126
Gedde-Dahl T Jr, Olaisen B, Teisberg P, Wilhelmy MC, Mevåg B, Helland R (1984) The locus for apolipoprotein E (apoE) is close to the Lutheran (Lu) blood group locus on chromosome 19. Hum Genet (in press)
Humphries SE, Williams L, Myklebost O, Stalenhoef AFH, Casparie AF, Baggia G, Galton DJ, Williamson R (1984a) Familial apolipoprotein CII deficiency: a preliminary analysis of the gene defect in two independent individuals. (In preparation)
Humphries SE, Jowett NI, Williams L, Rees A, Vella M, Kessling A, Mykeblost O, Lydon A, Seed M, Galton DJ, Williamson R (1984b) A DNA polymorphism adjacent to the human apolipoprotein CII gene. (In preparation)
Karathanasis SK, McPherson J, Zannis VI, Breslow JL (1983) Linkage of human apolipoproteins AI and CIII genes. Nature 304:371–373
Kunkel LM, Smith KD, Boyer SH, Borgaonkar DS Wachtel SS, Miller OJ, Breg WR, Jones HW Jr, Rary JM (1977) Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc Natl Acad Sci USA 74:1245–1249
La Rosa JC, Levy RI, Herbert P, Lux SE, Fredrickson DS (1970) Specific apoprotein activator for lipoprotein lipase. Biochem Biophys Res Commun 41:57–62
Lusis AJ, Taylor BA, Wangenstein RW, LeBoeuf RC (1983) Genetic control of lipid transport in mice. II: Genetic variation of high density lipoproteins. J Biol Chem 258:5071–5078
Miller NE, Rao SN, Alaupovic P, Noble N, Slack J, Brunzell JB, Lewis B (1981) Familial apolipoprotein CII deficiency: plasma lipoproteins and apolipoproteins in heterozygous and homozygous subjects and the effects of plasma infusions. Eur J Clin Invest 11:69–76
Morton NE (1955) Sequential tests for the detection of linkage. Am J Hum Genet 7:277–318
Myklebost O, Williamson R, Markham AF, Myklebost SR, Rogers J, Woods DE, Humphries SE (1984) The isolation and characterisa tion of cDNA clones for human apolipoproteins CII. J Biol Chem 259:4401–4404
Olaisen B, Teisberg P, Gedde-Dahl T Jr (1982) The locus for apolipoprotein E (apoE) is linked to the complement component C3 (C3) locus on chromosome 19 in man. Hum Genet 62:233–236
Olaisen B, Teisberg P, Jonassen R, Gedde-Dahl T Jr, Moen T, Thorsby E (1981) Complement loci of the HLA complex. Studies on families with intra-HLA cross-overs and haplotype associations. Hum Immunol 2:247–254
Schamaun O, Olaisen B, Mevåg B, Gedde-Dahl T Jr, Enholm C, Teisberg P (1984) The two apolipoprotein loci apoAI and apoAIV are closely linked in man. Hum Genet (in press)
Skolnick MH, Willard HF, Menlove LA (1984) Report of the committee on human gene mapping by recombinant DNA techniques. Cytogenet Cell Genet 37:210–248
Southern E (1979) Gel electrophoresis of restriction fragments Meth Enzymol 68:152–175
Utermann G, Hees M, Steinmetz A (1977) Polymorphism of apolipoprotein E and occurrence of dysbetalipoproteinaemia in man. Nature 269:604–607
Yamamura T, Sudo H, Ishikawa K Yamamoto A (1979) Familial type I hyperlipoproteinemia caused by apolipoprotein CII deficiency. Atherosclerosis 34:53–65
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Myklebost, O., Rogne, S., Olaisen, B. et al. The locus for apolipoprotein CII is closely linked to the apolipoprotein E locus on chromosome 19 in man. Hum Genet 67, 309–312 (1984). https://doi.org/10.1007/BF00291359
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00291359