Summary
Ag-staining of the nucleolus organizer region (NOR) was studied in the acrocentric chromosomes identified by Q-banding in repeated lymphocyte and skin fibroblast cultures from three different individuals. A similar pattern of Ag-stainability of NORs was found in the two tissues in each individual. Small differences concerning, in each case, only one of the acrocentric chromosomes were found between repeated lymphocyte cultures, as well as between lymphocyte and fibroblast cultures of the same individual without indication of any prevalence of one tissue type in a certain direction. The possibility that these differences are caused by different stages of NOR activation is discussed.
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Bloom, S. E., Goodpasture, C.: An improved technique for selective silver staining of nucleolar regions in human chromosomes. Hum. Genet. 34, 199–206 (1976)
Denton, T. E., Howell, W. M., Barret, J. K.: Human nucleolar organizer chromosomes: Satellite associations. Chromosoma 55, 81–84 (1976)
Evans, H. J., Buckland, R. A., Pardue, M. L.: Location of the genes coding for 18S and 28S ribosomal RNA in the human genome. Chromosoma 48, 405–426 (1974)
Goodpasture, C., Bloom, S. E.: Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53, 37–50 (1975)
Goodpasture, C., Bloom, S. E., Hsu, T. C., Arrighi, F. E.: Human nucleolar organizers: The satellites or the stalks? Am. J. Hum. Genet. 28, 559–566 (1976)
Henderson, A. S., Warburton, S., Atwood, K. C.: Localization of ribosomal DNA in the human chromosome complement. Proc. Natl. Acad. Sci. USA 69, 3394–3398(1972)
Howell, W. M., Denton, T. E., Diamond, J. R.: Differential staining of the satellite regions of human acrocentric chromosomes. Experientia 31, 260–262 (1975)
Mikelsaar, A.-V., Schmid, M., Krone, W., Schwarzacher, H. G., Schnedl, W.: Frequency of Ag-stained nucleolus organizer regions in the acrocentric chromosomes of man. Hum. Genet. 37, 73–77 (1977a)
Mikelsaar, A.-V., Schwarzacher, H. G., Schnedl, W., Wagenbichler, P.: Inheritance of Ag-stainability of nucleolus organizer regions. Investigations in 7 families with trisomy 21. Hum. Genet. 38, 183–188 (1977b)
Miller, D. A., Dev, V. G., Tantravahi, R., Miller, O. J.: Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp. Cell Res. 101, 235–243 (1976a)
Miller, O. J., Miller, A. D., Dev, V. G., Tantravahi, R., Croce, C. M.: Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. Proc. Natl. Acad. Sci. USA 73, 4531–4535 (1976b)
Münke, H., Schmiady, H.: Analyse des N-Bandenmusters von Interphasechromosomen mit unterschiedlicher RNA-Synthesereaktivität. Personal communication. Cited in: 15. Tag. Ges. Anthropol. Humangenetik, 1977
Schmid, M., Hofgärtner, F. J., Zenzes, M. T., Engel, W.: Evidence for post-meiotic expression of ribosomal RNA genes during male gametogenesis. Hum. Genet. (in press, 1978)
Schwarzacher, H. G., Mikelsaar, A.-V., Schnedl, W.: The nature of the Ag-staining of nucleolus organizer regions. Electron- and light-microscopic studies on human cells in interphase, mitosis and meiosis. Cytogenet. Cell Genet. (in press, 1978)
Varley, J. M.: Patterns of silver staining of human chromosomes. Chromosoma 61, 207–214 (1977)
Warburton, D., Henderson, A. S.: The relationship of number of rDNA copies per human acrocentric chromosome to satellite association and staining properties of the NO region. Personal communication. Cited in: Mammalian Chromosome Newsletter 18, 182 (1977)
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Mikelsaar, A.V., Schwarzacher, H.G. Comparison of silver staining of nucleolus organizer regions in human lymphocytes and fibroblasts. Hum Genet 42, 291–299 (1978). https://doi.org/10.1007/BF00291309
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DOI: https://doi.org/10.1007/BF00291309