Abstract
Subcellular distribution of 111In and 169Yb was quantitatively determined to evaluate the role of the lysosome in accumulation of these nuclides in malignant tumor tissue and in the liver using three different tumor models and the host liver. In Yoshida sarcoma and Ehrlich tumor, most of the radioactivity of these nuclides was localized in the supernatant fraction, and only a small amount of radioactivity was localized in the mitochondrial fraction, which contains lysosomes. In the liver, most of the radioactivity was concentrated in the mitochondrial fraction. The radioactivity of this fraction increased with time after the administration of these nuclides and reached approximately 50% of the total radioactivity within 24 h. In the case of hepatoma AH109A, radioactivity of the mitochondrial fraction increased with time after administration, and about 30% of the total radioactivity was concentrated in this fraction after 24 h. It is concluded that the lysosome does not play a major role in the tumor concentration of these nuclides, although it may play an important role in their liver concentration. In the case of hepatoma AH109A, it is presumed that lysosome plays a considerably important role in the tumor concentration of these nuclides, hepatoma AH109A possessing some residual features of the liver.
Similar content being viewed by others
References
Ando A, Doishita K, ando I, Sanada S, Hiraki T, Midsukami M, Hisada K (1978) Mechanism of tumor affinity of 169Yb, 67Ga and 111In-comparison of accumulation between viable tumor tissue and inflammatory infiltration around tumor by macroautoradiography. Abstracts of the second international congress of world federation of nuclear medicine and biology pp. 49. Washington DC, USA
Brown DH, Swartzendruber DC, Carlton JE, Byrd BL, Hayes RL (1973) The isolation and characterization of gallium-binding granules from soft tissue tumors. Cancer Res 33:2063–2066
Brown DH, Byrd BL, Carlton JE, Swartzendruber DC, Hayes RL (1976) A quantitative study of the subcellular localization of 67Ga. Cancer Res. 36:956–963
Deckner K, Becker G, Langowski U, Schwering H, Hornung G, Schmidt CG (1971) Die subcelluläre Bindung von 67-Gallium in Ascites-Tumorzellen. Z Krebsforsch 76:293–298
Edwards CL, Hayes RL (1969) Tumor scaning with 67Ga citrate. J Nucl Med 10:103–105
Hagan PL, Chauncey DM Jr, Halpern SE, McKegney ML (1977) Comparison of viable and nonviable tumor uptake of Sc-46, Mn-54, Zn-65, In-111 and Au-195 with Ga-67 citrate in a hepatoma model. Eur J Nucl Med 2:225–230
Hisada K, Ando A (1973) Radiolanthanides as promising tumor scanning agents. J Nucl Med 14:615–617
Hunter WW Jr, Dekock HW (1969) 111In for tumor localization (Abstract). J Nucl Med 10:343
Hunter WW Jr, Riccobono XJ (1970) Clinical evaluation of 111In for localization of recognized neoplastic disease (Abstract). J Nucl Med 11:328–329
Ito Y, Okuyama S, Sato K, Takahashi K, Sato T, Kanno I (1971) 67Ga tumor scanning and its mechanisms studied in rabbits. Radiology 100:357–362
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Orii H (1972) Tumor scanning with gallium (67Ga) and its mechanism studied in rats. Strahlentherapie 144:192–200
Swartzendruber DC, Nelson B, Hayes RL (1971) Gallium-67 localization in lysosomal-like granules of leukemic and nonleukemic murine tissues. J Natl Cancer Inst 46:941–952
Takeda S, Uchida T, Matsuzawa T (1977) A comparative study on lysosomal accumulation of gallium-67 and indium-111 in Morris hepatoma 7316A. J Nucl Med 18:835–839
Takeda S, Okuyama S, Takusagawa K, Matsuzawa T (1978) Lysosomal accumulation of gallium-67 in Morris hepatoma-7316A and Shionogi mammary carcinoma-115. Gann 69:267–271
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ando, A., Ando, I., Takeshita, M. et al. Subcellular distribution of 111In and 169Yb in tumor and liver. Eur J Nucl Med 6, 221–226 (1981). https://doi.org/10.1007/BF00290567
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00290567