Abstract
The dominant allele Gro1 confers on potato resistance to the root cyst nematode Globodera rostochiensis. The Gro1 locus has been mapped to chromosome VII on the genetic map of potato, using RFLP markers. This makes possible the cloning of Gro1 based on its map position. As part of this strategy we have constructed a high-resolution genetic map of the chromosome segment surrounding Gro1, based on RFLP, RAPD and AFLP markers. RAPD and RFLP markers closely linked to Gro1 were selected by bulked segregant analysis and mapped relative to the Gro1 locus in a segregating population of 1105 plants. Three RFLP and one RAPD marker were found to be inseparable from the Gro1 locus. Two AFLP markers were identified that flanked Gro1 at genetic distances of 0.6 cM and 0.8 cM, respectively. A genetic distance of 1 cM in the Gro1 region corresponds to a physical distance of ca. 100 kb as estimated by long-range restriction analysis. Marker-assisted selection for nematode resistance was accomplished in the course of constructing the high-resolution map. Plants carrying the resistance allele Gro1 could be distinguished from susceptible plants by marker assays based on the polymerase chain reaction (PCR).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barone A, Ritter E, Schachtschabel U, Debener T, Salamini F, Gebhardt C (1990) Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Mol Gen Genet 224:177–182
Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmid, R, Giraudat J, Leung J, Staskawicz BJ (1994) RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science 265:1856–1860
Burke D, Carte G, Olson G (1987) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236:806–812
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
Ganal MW, Young ND, Tanksley ST (1989) Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400
Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75
Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57
Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E (1993) Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato. Theor Appl Genet 85:541–544
Gebhardt C, Ritter E, Salamini F (1994) RFLP mapping of the potato. In: Phillips RL, Vasil IK (eds) DNA-based markers in plants. Kluwer Academic Publishers, Dordrecht pp 271–285
Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res 19:6553–6558
Kreike CM, de Koning JRA, Vinke JH, von Ooijen JW, Gebhardt C, Stiekema WJ (1993) Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Rol. Theor Appl Genet 87:464–470
Kreike CM, de Koning JRA, Vinke JH, von Ooijen JW, Stiekema WJ (1994) Quantitatively-inherited resistance to Globodera pallida is dominated by one major locus in Solanum spegazzinii. Theor Appl Genet 88:764–769
Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near isogenic lines. Proc Natl Acad Sci USA 88:2336–2340
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Mindrinos M, Katagiri F, Yu G-L, Ausubel FM (1994) The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell 78:1089–1099
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497
Meksem K, Leister D, Peleman J, Zabeau M, Salamini F, Gebhardt C. A high resolution map on potato chromosome V based on RFLP and AFLP markers. [of the vicinity of the R1 locus] Mol Gen Genet, this issue
Müller-Röber BT, Koßmann J, Hannah LC, Willmitzer L, Sonnewald U (1990) One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol Gen Genet 224:136–146
Niewöhner J, Salamini F, Gebhardt C (1995) Development of PCR assays diagnostic for RFLP marker alleles closely linked to alleles Gro1 and H1, conferring resistance to the root cyst nematode Globodera rostochiensis in potato. Mol Breed 1:65–78
Pineda O, Bonierbale MW, Plaisted RL, Brodie BB, Tanksley SD (1993) Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Genome 36:152–156
Ross H (1962) Über die Vererbung der Resistenz gegen den Kartoffelnematoden (Heterodera rostochiensis Wol.) in Kreuzungen von Solanum famatinae Bitt. et Wittm. mit Solamum tuberosum L. und mit S. chacoense Bitt. Der Züchter 32:74–80
Ross H (1986) Potato breeding — problems and perspectives. J Plant Breed Suppl 13
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, New York
Schumacher K, Ganal M, Theres K (1995) Genetic and physical mapping of the lateral suppressor (ls) locus in tomato. Mol Gen Genet, in press
Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I 2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185
Tanksley SD, Ganal MW, Prince JP, de Vincente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Vos P, Holgers R, Bleiker M, Reijans M, van der Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new concept for DNA fingerprinting. Nucleic Acids Res, in press
Whitham S, Dinesh-Kumar SP, Doil C, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to Toll and the interleukin-1 receptor. Cell 78:1101–1115
Williams JK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1991) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531–6535
Zabeau M, Vos P (1993) Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application, publication no: EP 0534858-A1
Author information
Authors and Affiliations
Additional information
Communicated by J. Schell
Rights and permissions
About this article
Cite this article
Ballvora, A., Hesselbach, J., Niewöhner, J. et al. Marker enrichment and high-resolution map of the segment of potato chromosome VII harbouring the nematode resistance gene Gro1 . Molec. Gen. Genet. 249, 82–90 (1995). https://doi.org/10.1007/BF00290239
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290239