Abstract
Differential staining of the core and RNP particles of RNP complexes in puff 2–48 BC in salivary gland chromosomes of Drosophila hydei was achieved with aqueous uranyl acetate (UA) at low pH, with UA in acetone, with phosphotungstic acid (PTA) in organic solvents, and with aqueous PTA at pH 5 and 6. A comparison of the results of UA and PTA staining under various conditions indicate that the proteins in the core region and in the RNP particles connected to it differ with respect to their amino-acid composition (arginine and lysine residues). — The staining mechanism of PTA and UA is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albrecht, C., Zijl, I. M. van: A comparative study of the protein components of ribonucleoprotein particles isolated from rat liver and hepatoma nuclei. Exp. Cell Res. 76, 8–14 (1973)
Beermann, W.: Chromomerenkonstanz und spezifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung von Chironomus tentans. Chromosoma (Berl.) 5, 139–198 (1952)
Beermann, W.: Nuclear differentiation and functional morphology of chromosomes. Cold Spr. Harb. Symp. quant. Biol. 21, 217–232 (1956)
Beermann, W., Bahr, G. F.: The submicroscopic structure of the Balbiani ring. Exp. Cell Res. 6, 195–201 (1954)
Berendes, H. D.: The control of puffing in Drosophila hydei. In: Developmental studies on giant chromosomes. Results and problems in cell differentiation, vol. 4 (W. Beermann, ed.), p. 181–207. Berlin-Heidelberg-New York: Springer 1972
Berendes, H. D., Meyer, G. F.: A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma (Berl.) 25, 184–197 (1968)
Bloom, F. E., Aghajanian, G. K.: Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid. J. Ultrastruct. Res. 22, 361–375 (1968)
Derksen, J.: The submicroscopic structure of synthetically active units in a puff of Drosophila hydei giant chromosomes. Chromosoma (Berl.) 50, 45–52 (1975)
Derksen, J., Berendes, H. D., Willart, E.: Production and release of a locus specific ribonucleoprotein product in polytene nuclei of Drosophila hydei. J. cell Biol. 59, 661–668 (1973)
Fujiwara, H., Kataoka, E.: Kritische Untersuchungen über die Folin-Denisschen kolorimetrischen Verfahren. Hoppe-Seylers Z. physiol. Chem. 216, 133–137 (1933)
Gabrusewycz-Garcia, N., Garcia, A. M.: Studies on the fine structure of puffs in Sciara coprophila. Chromosoma (Berl.) 47, 385–401 (1974)
Hamkalo, B. A., Miller, O. L. Jr.: Electron microscopy of genetic activity. Ann. Rev. Biochem. 42, 379–396 (1973)
Huxly, H. E., Zubay, G.: Electron microscope observations on the structure of microsomal particles from Escherichia coli, J. molec. Biol. 2, 10–18 (1960)
Leenders, H. J., Berendes, H. D.: The effect of changes in the respiratory metabolism upon genome activity in Drosophila. I. The induction of gene activity. Chromosoma (Berl.) 37, 433–444 (1972)
Leenders, H. J., Berendes, H. D., Helmsing, P. J., Derksen, J., Koninkx, J. P. J. G.: Nuclear mitochondrial interactions in the control of mitochondrial respiratory metabolism. Sub-Cell. Biochem. 3, 119–147 (1974)
Leenders, H. J., Derksen, J., Maas, P. M. J. M., Berendes, H. D.: Selective induction of a giant puff in Drosophila hydei by vitamin B6 and Derivates. Chromosoma (Berl.) 41, 447–460 (1973)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–41l4 (1961)
Martin, T., Billings, P., Levey, A., Ozarslan, S., Quinlan, T., Swift, H., Ulrbas, L.: Some properties of RNA; Protein complexes from the nucleus of eukaryotic cells. Cold Spr. Harb. Symp. quant. Biol. 38, 921–932 (1973)
Miller, O. L., Beatty, B. R.: Extrachromosomal nucleolar genes in amphibian oocytes. Genetics, Suppl. 61, 133–143 (1969)
Moelwyn-Hughes, E. A.: Physikalische Chemie. Stuttgart: Georg Thieme 1970
Monneron, A., Bernhard, W.: Fine structural organisation of the interphase nucleus in some mammalian cells. J. Ultrastruct. Res. 27, 266–288 (1969)
Palladini, G., Lauro, G., Basile, A.: Observations sur la spécificité de la coloration aux acides phosphotungstique et phosphomolybdique. Histochemie 24, 315–321 (1970)
Pease, D. C.: Polysaccharides associated with the exterior surface of epithelial cells: Kidney, intestine, brain. J. Ultrastruct. Res. 15, 555–588 (1966)
Poels, C. L. M.: Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity. Cell Differentiation 1, 63–78 (1972)
Quintarelli, G., Belloci, M., Geremia, R.: On phosphotungstic acid staining. IV. Selectivity of the staining reaction. J. Histochem. Cytochem. 21, 155–160 (1973)
Rambourg, A.: Staining of intracellular glycoproteins. In: Electron microscopy and cytochemistry (E. Wisse, W. Th. Daems, I. Molenaar and P. van Duyn, eds.), 245–247. Amsterdam, N. Y.: North Holland, American Elsevier 1973
Sarasin, A.: Particules ribonucléoproteiques 40 S des noyaux de foie de rat. Propriétés des protéins de ces particules. FEBS letters 4, 327–330 (1969)
Sheridan, W. E., Barnett, R. J.: Cytochemical studies on chromosome ultrastructure. J. Ultrastruct. Res. 27, 216–229 (1969)
Silverman, L., Glick, D.: The reactivity and staining of tissue proteins with phosphotungstic acid. J. Cell Biol. 40, 761–767 (1969)
Sommerville, J.: Ribonucleoprotein particles derived from the lampbrush chromosomes of newt oocytes. J. molec. Biol. 78, 487–503 (1973)
Stevens, B. J., Swift, H.: RNA transport from nucleus to cytoplasm in Chironomus salivary glands. J. Cell Biol. 31, 55–77 (1966)
Swift, H.: Nucleic acids and cell morphology in Dipteran salivary glands. In: The molecular control of cellular activity. (J. H. Allen, ed.), p. 73–125. New York: McGraw Hill Book Company 1962
Swift, H.: Cytochemical studies on nuclear fine structure. Exp. Cell Res. Suppl. 9, 54–67 (1963)
Swift, H.: Nuclear morphology of the chromosome. In Vitro 1, 26–49 (1965)
Vazquez-Nin, G., Bernhard, W.: Comparative ultrastructural study of perichromatin and Balbianiring granules. J. Ultrastruct. Res. 36, 842–860 (1971)
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965)
Zobel, C. R., Beer, M.: Electron stains. I. Chemical studies on the interactions of DNA with uranyl salts. J. Cell Biol. 10, 335–346 (1961)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Derksen, J., Willart, E. Cytochemical studies on RNP complexes produced by puff 2-48BC in Drosophila hydei . Chromosoma 55, 57–68 (1976). https://doi.org/10.1007/BF00288327
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00288327