Summary
A molecular probe containing a 584 base pairs sequence corresponding to part of the human calcitonin mRNA was used for the chromosomal assignment of the calcitonin gene. Restriction endonuclease analysis of DNA from human-Chinese hamster and human-mouse somatic cell hybrids, including some containing a translocation of human chromosomes, placed the calcitonin gene in the p14→qter region of chromosome 11.
Analysis of human DNA showed that the calcitonin gene has a polymorphic site for restriction endonuclease TaqI.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amara SG, Jones V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298:240–244
Craig RK, Hall L, Edbrooke MR, Allison J, MacIntyre I (1982) Partial nucleotide sequence of human calcitonin precursor mRNA identifies flanking cryptic peptides. Nature 295:345–347
Foster GV, Baghdiantz A, Kumar MA, Slack E, Soliman HA, Mac-Intyre I (1964) Thyroid origin of calcitonin. Nature 202:1303–1305
Geurts van Kessel AHM, Tetteroo PAT, von dem Borne AEGKr, Hagemeijer A, Bootsma D (1983) Expression of human myeloidassociated surface antigens in human-mouse myeloid cell hybrids. Proc Natl Acad Sci USA 80:3748–3752
Gross-Bellard M, Oudet P, Chambon P (1973) Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem 36:32–38
Grzeschik K-H (1973) Syrian hamster-human somatic cell hybrids: Isolation and characterization. Hum Genet 20:211–218
Herbschleb-Voogt E, Grzeschik K-H, Pearson PL, Meera Khan P (1981a) Assignment of adenosine deaminase complexing protein (ADCP) gene(s) to human chromosome 2 in rodent-human somatic cell hybrids. Hum Genet 59:317–323
Herbschleb-Voogt E, Pearson PL, Vossen JM, Meera Khan P (1981b) Basic defect in the expression of ADA in ADA-SCID disease investigated through the cells of an obligate heterozygote. Hum Genet 56:379–386
MacIntyre I, Hillyard CJ, Murphy PK, Reynolds JJ, Gaines-Das RE, Craig RK (1982) A second plasma calcium-lowering peptide from the human calcitonin precursor. Nature 300:460–462
Pearson PL, van Egmond-Cowan AMM (1976) Further development in banding techniques for mammalian chromosome identification. In: Pain RH, Smith BJ (eds) New techniques in biophysics and cell biology. Wiley, London, p 213
Rosenfeld MG, Mermod J-J, Amara SG, Swanson LW, Sawchencko PE, Rivier J, Vale WW, Evans RM (1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature 304:129–135
Stevenson JC, Hillyard CJ, MacIntyre I, Cooper H, Whitehead MI (1979) A physiological role for calcitonin: protection of the maternal skeleton. Lancet ii:769–770
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Höppener, J.W.M., Steenbergh, P.H., Zandberg, J. et al. Localization of the polymorphic human calcitonin gene on chromosome 11. Hum Genet 66, 309–312 (1984). https://doi.org/10.1007/BF00287635
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00287635