Summary
Five polymorphic DNA segments from human chromosome 3, that are frequently deleted in lung carcinoma were mapped by non-isotopic in situ hybridization to metaphase chromosomes. The DNA segment D3S3 mapped to 3p13–p14.2, D3S6 to 3p14.3–p14.5, D3S48 to distal 3p21–p22, ERBAß to 3p24.3 and ERBA2 to 3p24.3. The map location of ERBAß and ERBA2 was confirmed by re-mapping each probe in combination with D3S6 as a marker for 3p14.
Similar content being viewed by others
References
Albertson DG (1984) Localization of the ribosomal genes in Caenorhabditis elegans chromosomes by in situ hybridization using biotin-labeled probes. EMBO J 3:1227–1234
Albertson DG (1985) Mapping muscle protein genes by in situ hybridization using biotin labeled probes. EMBO J 4:2494–2498
Albertson DG, Fishpool R, Sherrington P, Nacheva E, Milstein C (1988) Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1. EMBO J 7:2801–2805
Bloomfield CD, Trent JM Berghe H van den (1987) Report of the committee on structural changes in neoplasia. (9th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 46:344–366
Brauch H, Johnson B, Hovis J, Yano T, Gazdar A, Pettengill OS, Graziano S, Sorenson GD, Poiesz BJ, Minna J, Linehan M, Zbar B (1987) Molecular analysis of the short arm of chromosome 3 in small-cell and non-small-cell carcinoma of the lung. N Engl J Med 317:1109–1113
Carritt B, Welch HM, Parry-Jones NJ (1986) Sequences homologous to the human D1S1 locus present on human chromosome 3. Am J Hum Genet 38:428–436
Dobrovic A, Houle B, Belouchi A, Bradley WEC (1988) erb-A-related sequence coding for DNA-binding hormone receptor localized to chromosome 3p21–3p25 and deleted in small cell lung carcinoma. Cancer Res 48:682–685
Donlon TA, Magenis RE (1984) Localization of the cloned segment λCh 4A-H3 to chromosome 1 band p36: a confirmation of locus D1S1. (7th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 37:454–455
Drabkin HA, Kao F-T, Weinberger C, Evans R (1987) Human c-erb-A is located at chromosome 3p21.33–p22. Am J Hum Genet 41:A25
Files JG, Hirsh D (1981) Ribosomal DNA of Caenorhabditis elegans. J Mol Biol 149:223–240
Gareau J-LP, Houle B, Leduc F, Bradley WEC, Dobrovic A (1988) A frequent HindIII RFLP on chromosome 3p21–3p25 detected by a genomic erbA sequence. Nucleic Acids Res 16:1223
Gerber MJ, Miller YE, Drabkin HA, Scoggin CH (1986) Regional assignment of the polymorphic probe D3S3 to 3p14 by molecular hybridization. Cytogenet Cell Genet 42:72–74
Goode ME, Tuinen P van, Ledbetter DH, Daiger SP (1986) The anonymous polymorphic DNA clone D1S1, previously mapped to human chromosome 1p36 by in situ hybridization, is from chromosome 3 and is duplicated on chromosome 1. Am J Hum Genet 38:437–446
Gosden JR, Middleton PG, Rout D, De Angelis C (1986) Chromosomal localization of the human oncogene ERBA2. Cytogenet Cell Genet 43:150–153
Gusella JF, Wasmuth JJ (1987) Report of the committee on the genetic constitution of chromosomes 3 and 4. (9th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 46:131–146
Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439
Icking D, Jandel AS, Kabisch R, Driesel AJ, Olek K, Grzeschik KH (1985) Human arbitrary fragments of genomic DNA detecting restriction fragment length polymorphisms on human chromosomes. (8th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 40:659
ISCN (1985) An international system for human cytogenetic nomenclature. Harnden DG, Klinger HP (eds) Published in collaboration with Cytogenet Cell Genet. Karger, Basel
Jansson M, Philipson L, Vennstrom B (1983) Isolation and characterization of multiple human genes homologous to the oncogenes of avian erythroblastosis virus. EMBO J 2:561–565
Kok K, Osinga J, Carritt B, Davis MB, Hout AH van der, Veen AY van der, Landsvater RM, Leij LFMH de, Berendsen HH, Postmus PE, Poppema S, Buys CHCM (1987) Deletion of a DNA sequence at the chromosomal region 3p21 in all major types of lung cancer. Nature 330:578–581
Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637
Martinville B de, Schafer M, White R, Francke U (1983) Chromosomal assignments of three random RFLP loci defined by basepair changes in MSPI sites. Mol Biol Med 1:415–424
Mattei M-G, The H de, Mattei J-F, Marchio A, Tiollais P, Dejean A (1988) Assignment of the human hap retinoic acid receptor RAR gene to the p24 band of chromosome 3. Hum Genet 80:189–190
Middleton PG, De Angelis CL, Weir-Thompson EM, Steel CM (1986) RFLP for the human erb A2 gene. Nucleic Acids Res 14:1925
Naylor SL, Johnson BE, Minna JD, Sakaguchi AY (1987) Loss of heterozygosity of chromosome 3p markers in small-cell lung cancer. Nature 329:451–454
Rider SH, Gorman PA, Shipley JM, Moore G, Vennstrom B, Solomon E, Sheer D (1987) Localization of the oncogene c-erbA2 to human chromosome 3. Ann Hum Genet 51:153–160
Weinberger C, Thompson CC, Ong ES, Lebo R, Gruol DJ, Evans RM (1986) The c-erb-A gene encodes a thyroid hormone receptor. Nature 324:641–646
Whang-Peng J, Bunn Jr PA, Kao-Shan CS, Lee EC, Carney DN, Gazdar A, Minna JD (1982) A non-random chromosomal abnormality del 3p(14–23), in human small cell lung cancer (SCLC). Cancer Genet Cytogenet 6:119–134
Wurster-Hill DH, Cannizzaro LA, Pettengill OS, Sorenson GD, Cate CC, Maurer LH (1984) Cytogenetics of small cell carcinoma of the lung. Cancer Genet Cytogenet 13:303–330
Yokota J, Wada M, Shimosato Y, Terada M, Sugimura T (1987) Loss of heterozygosity on chromosomes 3, 13, and 17 in small-cell carcinoma and on chromosome 3 in adenocarcinoma of the lung. Proc Natl Acad Sci USA 84:9252–9256
Yunis JJ, Sawyer JR, Ball DW (1978) The characterization of high-resolution G-banded chromosomes of man. Chromosoma 67: 293–307
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Albertson, D.G., Sherrington, P.D. & Rabbitts, P.H. Localization of polymorphic DNA probes frequently deleted in lung carcinoma. Hum Genet 83, 127–132 (1989). https://doi.org/10.1007/BF00286704
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00286704