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A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids

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Summary

A radioisotopic assay is described to distinguish between Chinese hamster and human steroid sulfatase activity in extracts prepared from hamsterxhuman somatic cell hybrids. This assay is based on different pH optima and provides a sensitive and unambiguous biochemical marker for the short arm of the human X chromosome and as well as for otherwise genetically inactivated X chromosomes in rodentxhuman hybrids.

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References

  • Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Bücher T, Bende W, Fundele R, Hofner H, Linke I (1980) Quantitative evaluation of electrophoretic allo-and isozyme patterns. FEBS Lett 115:319–324

    Google Scholar 

  • Chang PL, Davidson RG (1980) Complementation of arylsulfatase A in somatic hybrids of metachromatic leukodystrophy and multiple sulfatase deficiency disorder fibroblasts. Proc Natl Acad Sci USA 77:6166–6170

    Google Scholar 

  • Davidson RL, Gerald PS (1976) Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somatic Cell Genet 2:165–170

    Google Scholar 

  • Erickson RP, Harper K, Kramer JM (1983) Identification of an autosomal locus affecting steroid sulfatase activity among inbred strains of mice. Genetics 105:181–189

    Google Scholar 

  • Francke U, Oliver N (1978) Quantitative analysis of high resolution trypsin-Giemsa bands on numan prometaphase chromosomes. Hum Genet 45:137–165

    Google Scholar 

  • Gartler SM, Rivest M (1983) Evidence for X-linkage of steroid sulfatase in the mouse: steroid sulfatase levels in oocytes of XX and X0 mice. Genetics 103:137–141

    Google Scholar 

  • Grzeschik KH, Grzeschik AM, Banhof S, Romeo G, Siniscalco M, van Someran H, Meera Khan P, Westerveld A, Bootsma D (1972) X-linkage of human α-galactosidase. Nature 240:48–50

    Google Scholar 

  • Hameister H, Wolff G, Lavritzen CH, Lehmann WO, Hauser A, Ropers HH (1979) Clinical and biochemical investigations on patients with partial deficiency of placental steroid sulfatase. Hum Genet 46:199–207

    Google Scholar 

  • Migeon BR, Shapiro LJ, Norum RA, Mohandas T, Axelman J, Dabora RL (1982) Differential expression of steroid sulfatase locus on active and inactive human X chromosome. Nature 299: 838–841

    Google Scholar 

  • Mohandas T, Shapiro LJ, Sparkes RS, Sparkes MC (1979) Regional assignment of the steroid sulfatase-X-linked ichthyosis locus: implications for a noninactivated region on the short arm of human X chromosome. Proc Natl Acad Sci USA 76:5779–5783

    Google Scholar 

  • Mohandas T, Sparkes RS, Hellkuhl B, Grzeschik KH, Shapiro LJ (1980) Expression of an X-linked gene from an inactive human X chromosome in mouse-human hybrid cells: further evidence for the noninactivation of the steroid sulfatase locus in man. Proc Natl Acad Sci USA 77:6759–6763

    Google Scholar 

  • Müller CR, Westerveld A, Migl B, Franke W, Ropers HH (1980) Regional assignment of the gene locus for steroid sulfatase. Hum Genet 54:201–204

    Google Scholar 

  • Shapiro LJ (1983) Steroid sulfatase deficiency and X-linked ichthyosis. In: Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS (eds) The metabolic basis of inherited disease, 5th edn. McGraw-Hill Book Co, New York, pp 1027–1039

    Google Scholar 

  • Shapiro LJ, Mohandas T, Weiss R, Romeo G (1979) Noninactivation of an X-chromosome locus in man. Science 204:1224–1226

    Google Scholar 

  • Sparkes RS, Baluda MC, Townsend DE (1969) Cellulose acetate electrophoresis of human glucose-6-phosphate dehydrogenase. J Lab Clin Med 73:531–534

    Google Scholar 

  • Westerveld A, Visser RP, Meera Khan P, Bootsma D (1971) Loss of human genetic markers in man-Chinese hamster somatic cell hybrids. Nature 234:20–24

    Google Scholar 

  • Willard HF, Smith KD, Sutherland J (1983) Isolation and characterization of a major tandem repeat family from the human X chromosome. Nucleic Acids Res 11:2017–2033

    Google Scholar 

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Authors and Affiliations

  1. Department of Medical Genetics, University of Toronto, M5S 1A8, Toronto, Ontario, Canada

    H. F. Willard & Maria Tzetis Holmes

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  1. H. F. Willard
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  2. Maria Tzetis Holmes
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Willard, H.F., Holmes, M.T. A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids. Hum Genet 66, 272–275 (1984). https://doi.org/10.1007/BF00286615

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  • DOI: https://doi.org/10.1007/BF00286615

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