Summary
Well-spread human pachytene spermatocyte bivalents were obtained allowing specific identification of each bivalent within its total complement according to its chromomere sequence combined with further staining of its centromeric heterochromatin. The total number of chromomeres was found to be related to the degree of bivalent contraction: 396 in condensed bivalents and 511 in decondensed bivalents. A striking correspondance between chromomeres and mitotic G-bands was observed; on account of the variability of bivalent contraction, condensed bivalents corresponded to prometaphase somatic chromosomes and decondensed bivalents to mid/late prophase chromosomes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Francke U, Oliver N (1978) Quantitative analysis of high resolution trypsin-Giemsa bands of human prometaphase chromosomes. Hum Genet 45:137–165
Hungerford DA, Hungerford AM (1978) Chromosome structure and function. IV. Pachytene chromomere maps of 16, 17 and 18; pachytene as a reference standard for metaphase banding. Cytogenet Cell Genet 21:212–230
Hungerford DA, La Badie GU, Balaban GB (1971) Chromosome structure and function in man. II. Provisional maps of the two smallest autosomes (chromosome 21 and 22) at pachytene in the male. Cytogenetics 10:33–37
ISCN (1981) An international system for human cytogenetic nomenclature: high resolution banding. Cytogenet Cell Genet 31:5–18
Jagiello GM, Fang JS (1982) Complete autosomal chromomere maps of human early and mid/late pachytene spermatocytes. Am J Hum Genet 34:112–124
Jhanwar SC, Burns JP, Alonso ML, Hew W, Chaganti RSK (1982) Mid-pachytene chromomere maps of human autosomes. Cytogenet Cell Genet 33:240–248
Luciani JM, Morazzani MR, Stahl A (1975) Identification of pachytene bivalents in human male meiosis using G-banding technique. Chromosoma 52:275–282
Luciani JM, Devictor M, Morazzani MR, Stahl A (1976) Meiosis of trisomy 21 in the human pachytene oocyte. Chromosoma 57: 155–163
Luciani JM, Devictor M, Morazzani MR, Stahl A (1977) Pachytene mapping of the C9 and acrocentric bivalents in the human oocyte. Hum Genet 36:197–204
Luciani JM, Devictor M, Boue J, Morazzani MR, Boue A, Stahl A (1978) Etude de la méiose ovocytaire chez un foetus trisomique 18. Comportement du chromosome surnuméraire et identification du bivalent 18. Ann Génét (Paris) 21:215–218
Oud JL, Reutlinger AHH (1981) Chromosome behaviour during early meiotic prophase of mouse primary spermatocytes. Chromosoma 83:395–407
Solari AJ (1980) Synaptonemal complexes and associated structures in microspread human spermatocytes. Chromosoma 81:315–337
Yunis JJ (1981) Mid-prophase human chromosomes. The attainment of 2000 bands. Hum Genet 56:293–298
Yunis JJ, Sawyer JR, Ball DW (1978) The characterization of high resolution G-banded chromosomes of man. Chromosoma 67: 293–307
Yunis JJ, Ball DW, Sawyer JR (1979) G-banding patterns of high resolution human chromosomes 6-22, X and Y. Hum Genet 49:291–306
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luciani, J.M., Guichaoua, M.R. & Morazzani, M.R. Complete pachytene chromomere karyotypes of human spermatocyte bivalents. Hum Genet 66, 267–271 (1984). https://doi.org/10.1007/BF00286614
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00286614