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Completion of the Norwalk virus genome sequence

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Abstract

Norwalk virus (NV) is the prototype human calicivirus, and causes epidemic outbreaks of acute gastroenteritis. The sequence and predicted genome organization of NV and a NV-like virus [Southampton virus (SHV)] suggested they are similar viruses at the nucleotide and amino acid level, although SHV was reported to be antigenically distinct from NV. A recent review described the discovery of an additional 12 nucleotides at the 5′ end of SHV and prompted us to investigate the possibility of additional nucleotides at the 5′ end of the NV genome. The results obtained by homopolymeric tailing of NV cDNA with dCTP and dATP showed 12 additional nucleotides also are present on the NV genomic RNA. These data are important with respect to the biology of the virus, and make the genome sequence of NV complete.

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References

  1. Estes M.K. and Hardy M.E. in Blaser M., Smith P., Ravdin J., Greenberg H.B., and Guerrant R. (eds) Infections of the gastrointestinal tract. Raven Press, New York, 1995, pp. 1009–1034.

    Google Scholar 

  2. Kapikian A.Z., Estes M.K., and Chanock R.M., in Fields B.N., Knipe D.M., and Howley P.M. (eds) Fields Virology. Raven Press, New York, 1996, pp. 783–810.

    Google Scholar 

  3. Jiang X., Graham D.Y., Wang K.N., and Estes M.K., Science 250, 1580–1583. 1990.

    Google Scholar 

  4. Matsui S.M., Kim J.P., Greenberg H.B., Su W., Sun Q., Johnson P.C., Dupont H.L., Oshiro L.S., and Reyes G.R., J Clin Invest 87, 1456–1461, 1991.

    Google Scholar 

  5. Jiang X., Wang M., Wang K., and Estes M.K., Virology 195, 51–61, 1993.

    Google Scholar 

  6. Lambden P.R., Caul E.O., Ashley C.R., and Clarke I.N., Science 259, 516–519, 1993.

    Google Scholar 

  7. Palmenberg A.C. in Semler B.L., and Ehrenfeld E., (eds) Molecular aspects of picornavirus infection and detection. American Society for Microbiology. Washington, D.C. 1989, pp. 211–241.

    Google Scholar 

  8. Lambden P.R. and Clarke I.N., Trends in Microbiol 3, 261–265, 1995.

    Google Scholar 

  9. Lambden P.R., Liu B.L. and Clarke I.N., Virus Genes 10, 149–152, 1995.

    Google Scholar 

  10. Kozak M., Nucl Acids Res, 9, 5233–5252, 1981.

    Google Scholar 

  11. Neill J.D., Reardon I.M., and Heinrikson R.L., J Virol 65, 5440–5447, 1991.

    Google Scholar 

  12. Carter M.J. Arch Virol 114, 143–152, 1990.

    Google Scholar 

  13. Neill J.D. and Mengeling W.L., Virus Res 11, 59–72, 1988.

    Google Scholar 

  14. Meyers G., Wirblich C., and Thiel H.J., Virology, 184, 677–686, 1991.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Division of Molecular Virology, Baylor College of Medicine, One Baylor Plaza, 77030, Houston, TX, USA

    Michele E. Hardy & Mary K. Estes

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  1. Michele E. Hardy
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  2. Mary K. Estes
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Hardy, M.E., Estes, M.K. Completion of the Norwalk virus genome sequence. Virus Genes 12, 287–290 (1996). https://doi.org/10.1007/BF00284649

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  • DOI: https://doi.org/10.1007/BF00284649

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