Summary
The ptsG gene of Bacillus subtilis encodes Enzyme IIG1c of the phosphoenolpyruvate: glucose phosphotransferase system. The 3′ end of the gene was previously cloned and the encoded polypeptide found to resemble the Enzymes IIIGlc of Escherichia coli and Salmonella typhimurium. We report here cloning of the complete ptsG gene of B. subtilis and determination of the nucleotide sequence of the 5′ end. These results, combined with the sequence of the 3′ end of the gene, revealed that ptsG encodes a protein consisting of 699 amino acids and which is similar to other Enzymes II. The N-terminal domain contains two small additional fragments, which share no similarities with the closely related Enzymes IIGlc and IINag of E. coli but which are present in the IIG1c-like protein encoded by the E. coli malX gene.
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References
Blatch GL, Scholle RR, Woods DR (1990) Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region. Gene 95:17–23
Erni B, Zanolari B (1986) Glucose permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIG1c. J Biol Chem 262:16398–16403
Fouet A, Arnaud M, Klier A, Rapoport G (1987) Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: Expression in Escherichia coli and homology to enzymes II from enteric bacteria. Proc Natl Acad Sci USA 84:8773–8777
Gonzy-Tréboul G, Zagorec M, Rain-Guion M-C, Steinmetz M (1989) Phosphoenolpyruvate: sugar phosphotransferase system of Bacillus subtilis: nucleotide sequence of ptsX, ptsH and the 5′ end of pts1 and evidence for a ptsH1 operon. Mol Microbiol 3:103–112
Gonzy-Tréboul G, de Waard JH, Zagorec M, Postma PW (1991) The glucose permease of the phosphotransferase system of Bacillus subtilis: evidence for IIGlc and IIGlc domains. Mol Microbiol 5:1241–1249
Gonzy-Tréboul G, Karmazyn-Campelli C, Stragier P (1992) Developmental regulation of transcription of the Bacillus subtilis ftsAZ operon. J Mol Biol 224:967–979
Lengeler JW, Titgemeyer F, Vogler AP, Wöhrl BM (1990) Structures and homologies of carbohydrate: phosphotransferase system (PTS) proteins. Phil Trans R See Lend [B] 326:489–504
Lopilato J, Bortner S, Beckwith J (1986) Mutation of a new chromosomal gene of Escherichia coli K12, penB, reduces plasmid copy number of pBR322 and its derivatives. Mol Gen Genet 205:285–290
Postma PW, Lengeler JW (1985) Phosphoenolpyruvate: carbohydrate phosphotransferase system of bacteria. Microbiol Rev 49:232–269
Poth H, Youngman P (1988) A new system for Bacillus subtilis comprising elements of phage, plasmid and transposon vectors. Gene 73:215–226
Reidl J, Boos W (1991) The malX malY operon of Escherichia coli encodes a novel Enzyme 11 of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system. J Bacteriol 173:4862–4876
Rogers MJ, Ohgi T, Plumbridge J, Sö11 D (1988) Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate:sugar phosphotransferase system and for glucosamine-6-phosphate deaminase. Gene 62:197–207
Stragier P, Bonamy C, Karmazyn-Campelli C (1988) Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression. Cell 52:697–704
Sutrina SL, Reddy P, Saier MH, Reizer J (1990) The glucose permease of Bacillus subtilis is a single polypeptide chain that functions to energize the sucrose permease. J Biel Chem 265:18581–18589
Youngman P (1987) Plasmid vectors for recovering and exploiting Tn917 transpositions in Bacillus subtilis and other Grampositive bacteria. In: Hardy K (ed) Plasmds: a practical approach. IRL Press, Oxford, pp 79–103
Youngman P, Poth H, Green B, York K, Olmedo G, Smith K (1989) Methods for genetic manipulation, cloning and functional analysis of sporulation genes in Bacillus subtilis. In: Smith I, Slepecky R, Setlow P (eds) Regulation of prokaryotic development. American Society for Microbiology, Washington, pp 65–87
Zukowski MM, Miller L, Cogswell P, Chen K, Aymerich A, Steinmetz M (1990) Nucleotide sequence of the sacS locus of Bacillus subtilis reveals the presence of two regulatory genes. Gene 90:153–155
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Communicated by J. Lengeler
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Zagorec, M., Postma, P.W. Cloning and nucleotide sequence of the ptsG gene of Bacillus subtilis . Molec. Gen. Genet. 234, 325–328 (1992). https://doi.org/10.1007/BF00283853
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DOI: https://doi.org/10.1007/BF00283853