Abstract
The gene fimU, located on a recombinant plasmid carrying the Salmonella typhimurium type 1 fimbrial gene cluster is closely related to the Escherichia coli tRNA gene argU. The fimU gene complements an E. coli argU mutant that is a P2 lysogen, thereby allowing the phage P4 to grow in this strain but preventing the growth of phage lambda. In addition, fimU was shown to be involved in fimbrial expression since transformants of the E. coli argU mutant could produce fimbriae only in the presence of fimU but not in its absence, whereas in an E. coli argU + strain fimbriation did not require the fimU gene.
References
Blomfield IC, Calie PJ, Eberhardt KJ, McClain MS, Eisenstein BI (1993) Lrp stimulates phase variation of type 1 fimbriation in Escherichia coli K-12. J Bacteriol 175:27–36
Clegg S, Swenson DL, Convery L (1993) Characterization of the genes encoding type 1 fimbriae of Salmonella typhimurium. Abstr D105. Abstr 93rd Annual Meeting Am Soc Microbiol p 114
Clegg S, Hull S, Hull R, Pruckler J (1985) Construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the Enterobacteriaceae. Infect Immun 48:275–279
Cohen P, Newman J, Laux D (1993) Characterization of genes which enhance intestinal colonization by Escherichia coli. Abstr D-54. Abstr 93rd Annual Meeting Am Soc Microbiol p 104
Dorman CJ, Higgins CF (1987) Fimbrial phase variation in Escherichia coli: dependence on integration host factor and homologies with other site-specific recombinases. J Bacteriol 169:3840–3843
Duguid, JP, Campbell I (1969) Antigens of the type-1 fimbriae of salmonellae and other enterobacteria. J Med Microbiol 2:535–553
Ghisotti D, Finkel S, Halling C, Dehoe G, Sironi G, Calender R (1990) Nonessential region of bacteriophage P4: DNA sequence, transcription, gene products, and function. J Virol 64:24–36
Ghisotti D, Zangrossi S, Sironi G (1983) An Escherichia coli gene required for bacteriophage P2-λ interference. J Virol 48:616–626
Lingquist BL, Lebenthal E, Lee PC, Stinson MW, Merrick JM (1987) Adherence of Salmonella typhimurium to small intestinal enterocytes of the rat. Infect Immun 55:3044–3050
Lu C-D, Abdelal AT (1993) The Salmonella typhimurium uracil-sensitive mutation use is in argU and encodes a minor arginine tRNA. J Bacteriol 175:3897–3899
McClain MS, Blomfield IC, Eisenstein BI (1991) Roles of fimB and fimE in site-specific DNA inversion associated with phase variation of type 1 fimbriae in Escherichia coli. J Bacteriol 173:5308–5314
Old DC, Duguid JP (1970) Selective outgrowth of fimbriate bacteria in static liquid media. J Bacteriol 103:447–456
Sanderson KE, Roth JR (1988) Linkage map of Salmonella typhimurium, edition VII. Microbiol Rev 52:485–532
Six EW, Klug CAC (1973) Bacteriophage P4: a satellite virus depending on a helper such as prophage P2. Virology 51: 327–344
Swenson DL, Clegg S (1992) Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium. J Bacteriol 174:7697–7704
Swenson DL, Clegg S (1994) Salmonella fimbriae. In: Klemm P (ed) Fimbriae: adhesion, biogenesis, genetics and vaccines. CRC Press, Boca Raton, Fla (in press)
Tavendale A, Jardine CKH, Old DC, Duguid JP (1983) Haemagglutinins and adhesion of Salmonella typhimurium to Hep2 and HeLa cells. J Med Microbiol 16:371–380
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Communicated by W. Goebel
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Swenson, D.L., Kim, KJ., Six, E.W. et al. The gene fimU affects expression of Salmonella typhimurium type 1 fimbriae and is related to the Escherichia coli tRNA gene argU . Molec. Gen. Genet. 244, 216–218 (1994). https://doi.org/10.1007/BF00283525
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DOI: https://doi.org/10.1007/BF00283525