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Glucose repression in Streptomyces coelicolor A3(2): a likely regulatory role for glucose kinase

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Abstract

The glucose kinase gene (glkA-ORF3) of Streptomyces coelicolor A3(2) plays an essential role in glucose utilisation and in glucose repression of a variety of genes involved in the utilisation of alternative carbon sources. These genes include dagA, which encodes an extracellular agarase that permits agar utilisation. Suppressor mutants of glkA-ORF3 deletion strains capable of utilising glucose (Glc+) arise at a frequency of about 10−5 on prolonged incubation. The Glc+ phenotype of the mutants is reversible (at a frequency of about 10−3) and reflects either the activation of a normally silent glucose kinase gene or the modification of an existing sugar kinase. Although the level of glucose kinase activity in the Glc+ supressor mutants is similar to that in the glkA + parental strain, glucose repression of dagA remains defective. Expression of the glucose kinase gene of Zymomonas mobilis in glkA-ORF3 mutants restored glucose utilisation, but not glucose repression of dagA. Over-expression of glkA-ORF3 on a high-copy-number plasmid failed to restore glucose repression of dagA in glkA-ORF3 mutants and led to loss of glucose repression of dagA in a glkA + strain. These results suggest that glucose phosphorylation itself is not sufficient for glucose repression and that glkA-ORF3 plays a specific regulatory role in triggering glucose repression in S. coelicolor A3(2).

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Author notes

  1. Susan Angell & Cinzia G. Lewis

    Present address: The Sainsbury Laboratory, Norwich Research Park, NR47UH, Colney, Norwich, UK

Authors and Affiliations

  1. John Innes Centre, NR4 7UH, Colney, Norwich, UK

    Susan Angell, Cinzia G. Lewis, Mark J. Buttner & Mervyn J. Bibb

Authors
  1. Susan Angell
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  2. Cinzia G. Lewis
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  3. Mark J. Buttner
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  4. Mervyn J. Bibb
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Communicated by A. Kondorosi

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Angell, S., Lewis, C.G., Buttner, M.J. et al. Glucose repression in Streptomyces coelicolor A3(2): a likely regulatory role for glucose kinase. Molec. Gen. Genet. 244, 135–143 (1994). https://doi.org/10.1007/BF00283514

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  • DOI: https://doi.org/10.1007/BF00283514

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