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Isozyme patterns and cellular differentiation in Schizophyllum

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Summary

Isozyme patterns of four enzymes were examined from two types of differentiated cells of Schizophyllum commune. Previous studies (Wang and Raper, 1970) demonstrated dramatically different isozyme patterns for each of these enzymes; however, the studies reported here reveal no isozyme differences for these enzymes previously correlated to cellular differentiation. Possible explanations for thesediscrepancies are discussed. These results illustrate difficulties inherent in ascribing differences in cellular proteins or nucleic acids to differentiation.

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References

  • Adatthody, K.K., Racusen, D.: On the extraction of peroxidase isozymes from bean leaves. Canad. J. Botany 45, 2237–2242 (1967)

    Google Scholar 

  • Davis, B.J.: Disc electrophoresis—II Method and application to human serum proteins. Ann. N.Y. Acad.Sci. 121, 404–427 (1964)

    Google Scholar 

  • Fairbanks, G., Steck, T.L., Wallach, D.F.H.: Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochem. 10, 2606–2617 (1971)

    Google Scholar 

  • Fawole, M.O., Casselton, P.J.: Observations on the regulation of glutamate dehydrogenase activity in Coprinus lagopus. J. exp. Bot. 23, 530–551 (1972)

    Google Scholar 

  • Gornall, A.G., Bardawill, C.J., David, M.M.: Determination of serum proteins by means of the biuret reaction. J. biol. Chem. 177, 751–766 (1949)

    Google Scholar 

  • Hoffman, R.M., Raper, J.R.: Genetic restriction of energy conservation in Schizophyllum. Science 171, 418–419 (1971)

    Google Scholar 

  • Hoffman, R.M., Raper, J.R.: Lowered respiratory response to ADP of mitochondria isolated from a mutant-β strain of Schizophyllum commune. J. Bact. 110, 780–781 (1972)

    Google Scholar 

  • Kapoor, M., Grover, A.K.: Catabolite-controlled regulation of glutamate dehydrogenases of Neurospora crassa. Canad. J. Microbiol. 16, 33–40 (1970)

    Google Scholar 

  • Raper, J.R., Esser, K.: Antigenic differences due to the incompatibility factors in Schizophyllum commune. Z. Vererbungsl. 92, 439–444 (1961)

    Google Scholar 

  • Raper, J.R., Hyatt, E.A.: Modified press for disruption of microorganisms. J. Bact 85, 712–713 (1963)

    Google Scholar 

  • Ross, I.K., Martin, E.M., Thoman, M.: Changes in isozyme patterns between monokaryons and dikaryons of a bipolar Coprinus. J. Bact. 114, 1083–1089 (1973)

    Google Scholar 

  • Schwalb, M.N., Miles, P.G.: Morphogenesis of Schizophyllum commune. I. Morphological variation and mating behavior of the thin mutation. Amer. J. Bot 54, 440–446 (1967)

    Google Scholar 

  • Wang, C-S., Raper, J.R.: Protein specificity and sexual morphogenesis in Schizophyllum commune. J. Bact. 99, 291–297 (1969)

    Google Scholar 

  • Wang, C-S., Raper, J.R.: Isozyme patterns and sexual morphogenesis in Schizophyllum. Proc. nat. Acad. Sci. (Wash.) 66, 882–889 (1970)

    Google Scholar 

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Communicated by F. Kaudewitz

Vermont Agricultural Experiment Station Journal Article No. 375

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Ullrich, R.C. Isozyme patterns and cellular differentiation in Schizophyllum . Molec. Gen. Genet. 156, 157–161 (1977). https://doi.org/10.1007/BF00283488

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