Summary
We have used a cDNA clone for human phosphoglycerate kinase (PGK) to examine the chromosomal localization of three members of the human PGK gene family. Using somatic cell hybrids segregating portions of several X-autosome translocations as well as a clone panel of hybrids segregating radiation-induced fragments of the human X chromosome, we assign a PGK pseudogene to the region Xq11–Xq13, proximal to the functional X-linked PGK gene located in Xq13. In addition, using a panel of 24 somatic cell hybrids, we assign an autosomal PGK-related DNA sequence to human chromosome 19.
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Becker MA, Yen RCK, Itkin P, Goss SJ, Seegmiller JE, Bakay B (1979) Regional localization of the gene for human phosphoribosylpyrophosphate synthetase on the X chromosome. Science 203:1016–1019
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331
Buck DW, Goss SJ, Bodmer WF (1976) Serological identification of an X-linked human cell surface antigen. Cytogenet Cell Genet 16:99–101
Chen SH, Donahue RP, Scott CR (1976) Characterization of phosphoglycerate kinase from human spermatozoa. Fertil Steril 27:699–701
Eicher EM, Cherry M, Flaherty L (1978) Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex. Mol Gen Genet 158:225–228
Goss SJ, Harris H (1975) New method for mapping genes in human chromosomes. Nature 255:680–684
Goss SJ, Harris H (1977) Gene transfer by means of cell fusion. I. Statistical mapping of the human X chromosome by analysis of radiation-induced gene segregation. J Cell Sci 25:17–37
Jolly DJ, Okayama H, Berg P, Esty AC, Filipula D, Bohlen P, Johnson GG, Shively JE, Hunkapillar T, Friedmann T (1983) Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyltransferase. Proc Natl Acad Sci USA 80:477–481
Korneluk RG, Quan F, lewis WH, Guise KS, Willard HF, Holmes MT, Gravel RA (1984) Isolation of human fibroblast catalase cDNA clones. J Biol Chem 259:13819–13823
Meera Khan P (1971) Enzyme electrophoresis on cellulose acetate gel: Zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybrids. Arch Biochem Biophys 145:470–483
Michaelson AM, Markham AF, Orkin SH (1983) Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc Natl Acad Sci USA 80:472–476
Michaelson AM, Bruns GAP, Morton CC, Orkin SH (1985) Human phosphoglycerate kinase multigene family: HLA-associated sequences and an X-linked locus containing a processed pseudogene and its functional counterpart. J Biol Chem 260:6982–6992
Miller OJ, Drayna D, Goodfellow P (1984) Report of the committee on the genetic constitution of the X and Y chromosomes. Cytogenet Cell Genet 37:176–204
Ricciuti F, Ruddle FH (1973) Assignment of nucleoside phosphorylase to D-14 and localization of X-linked loci im man by somatic cell genetics. Nature 241:180–182
Rubin JS, Prideaux VR, Willard HF, Dulhanty AM, Whimore GF, Bernstein A (1985) Molecular cloning and chromosomal localization of DNA sequences associated with a human DNA repair gene. Mol Cell Biol 5:398–405
Singer-Sam J, Simmer RL, Keith DH, Shively L, Teplitz M, Itakura K, Gartler SM, Riggs AD (1983) Isolation of a cDNA clone for human X-linked phosphoglycerate kinase by use of a mixture of synthetic oligodeoxyribonucleotides as a detection probe. Proc Natl Acad Sci USA 80:802–806
Szabo P, Grzeschik KH, Siniscalco M (1984) A human autosomal phosphoglycerate kinase locus maps near the HLA cluster. Proc Natl Acad Sci USA 81:3167–3169
Willard HF (1985) Chromosome-specific organization of human alpha satellite DNA. Am J Hum Genet 37:524–533
Willard HF, Holmes MT (1984) A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids. Hum Genet 66:272–275
Willard HF, Smith KD, Sutherland J (1983) Isolation and characterization of a major tandem repeat family from the human X chromosome. Nucleic Acids Res 11:2017–2033
Willard HF, Schmeckpeper BJ, Smith KD, Goss SJ (1984) High resolution regional mapping of the human X chromosome by radiation-induced segregation analysis of X-linked DNA probes. Cytogenet Cell Genet 37:609–610
Willard HF, Meakin S, Tsui LC, Breitman M (to be published) Assignment of the human gamma crystallin multigene family to chromosome 2. Somatic Cell Mol Genet
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Willard, H.F., Goss, S.J., Holmes, M.T. et al. Regional localization of the phosphoglycerate kinase gene and pseudogene on the human X chromosome and assignment of a related DNA sequence to chromosome 19. Hum Genet 71, 138–143 (1985). https://doi.org/10.1007/BF00283369
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DOI: https://doi.org/10.1007/BF00283369