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Sensitivity tests of tumors to cytostatic agents

Sensibilitätstestung von Tumoren gegenüber Cytostatica. II. Untersuchungen an menschlichen Tumoren

II. Investigation on human tumors

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Summary

Certain substances which influenced nucleic acid metabolism were found to have about the same cytostatic activity on human tumor cells when measured in tissue culture experiments (cell numbers) or in short-term cultures (3H-uridine incorporation in cell suspensions). By treatment with a dose of cytostatics corresponding to 10 times therapeutic dose, chemosensitive tumors can be distinguished from non-responsive tumors. By using this in vitro test system to investigate the sensitivities of 100 human tumors, it is shown that 28 of these tumors were responsive to adriamycin, daunomycin and Actinomycin D. Good agreement was observed between these in vitro results and the literature data on clinical therapy using these particular substances.

Zusammenfassung

Es wird gezeigt, daß verschiedene direkt auf den Nukleinsäurestoffwechsel wirkende Substanzen auf menschliche Tumorzellen in der Gewebekultur (Bestimmung der Zellzahl) und in der Kurzzeitkultur (Bestimmung des 3H-Uridin-Einbaus bei Zellsuspensionen) gleichermaßen wirksam sind. Bei einer 10fach therapeutischen Dosierung lassen sich chemoempflndliche von chemounempflndlichen Tumoren unterscheiden. Im beschriebenen in vitro-Test erweisen sich bei einer 10fach therapeutischen Dosierung 28 von 100 menschlichen Tumoren gegenüber den untersuchten Substanzen Adriamycin, Daunomycin und Actinomycin D als empfindlich. Ein Vergleich dieser in viro-Ergebnisse mit den in der Literatur beschriebenen Therapieerfolgen mit diesen Cytostatika zeigt eine gute Übereinstimmung.

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References

  • Cobb,U.P., Walker,D.G., Wright,J.C.: Comparative chemotherapy studies on primary shortterm cultures of human normal, benign and malignant tumor tissue —a five year study. Cancer Res. 21, 583–590 (1961)

    Google Scholar 

  • Barlow,J.J., Piver,M.S., Chuang,J.T., Cortes,E.P., Ohnuma,T., Holland,J.F.: Adriamycin and bleomycin, alone and in combination, in gynecologic cancers. Cancer 32, 735–743 (1973)

    Google Scholar 

  • Gottlieb,J.A., Rivkin,S.E., Spigel,S.C., Hoogstraten,B., O'Bryan,R.M., Delaney,F.C., Singhakowinta,A.: Superiority of adriamycin over oral nitrosoureas in patients with advanced breast carcinoma. Cancer 33, 519–526 (1974)

    Google Scholar 

  • Kenis,Y., Michel,J. Rimoldi,R., Israel,L., Levy,P.: Results of a clinical trial with intermittent doses of adriamycin in lung cancer. Europ. J. Cancer 8, 485–489 (1972)

    Google Scholar 

  • Limburg,H., Krahe,M.: Die Züchtung von menschlichem Krebsgewebe in der Gewebekultur und seine Sensibilitätstestung gegen neuere Cytostatika. Dtsch. med. Wschr. 89, 1938–1946 (1964)

    Google Scholar 

  • Livingston,R.B., Carter,S.K.: Single agents in cancer chermotherapy. New York: IFI/Plenum Press 1970

    Google Scholar 

  • O'Bryan,R.M., Luce,J.K., Talley,R.W., Gottlieb,J.A., Baker,L.H., Bonadonna,G.: Phase II evaluation of adriamycin in human neoplasia. Cancer 32, 1–8 (1973)

    Google Scholar 

  • Rozman,C., Camps,E.S., Mundo,M.R., Solsona,F., Dantart,I., Raichs,A., Giralt,M.: Clinical trial of Adriamycin. In: Carter,S.K., Di Marco,A., Ghione,M., Krakoff,I.H., Mathe,G. (Eds.): International symposium on adriamycin, pp. 188–194. Berlin-Heidelberg-New York: Springer 1972

    Google Scholar 

  • Selawry,O.S.: Monochemotherapy of bronchogenic carcinoma with special reference to cell type. Cancer chemother. Rep. 4, 177–188 (1973)

    Google Scholar 

  • Skipper,H.E., Hutschison,D.J., Schabel,F.M., Schmidt,L.H., Goldin,A., Brockman,R.W., Venditti,J.M., Wodinsky,I.: A quick reference chart on cross resistance between anticancer agents. Cancer chermother. Rep. 56, 493 (1972)

    Google Scholar 

  • Smith,J.P., Rutledge,F., Wharton,J.T.: Chemotherapy of ovarian cancer. Cancer 30, 1565–1571 (1972)

    Google Scholar 

  • Tanneberger St., Bacigalupo,G.: Die Benutzung von Zellkulturen zur Ermittlung der Sensibilität menschlicher Tumoren gegenüber Cytostatika. Dtsch. Gesundh.-Wes. 22, 11 (1967)

    Google Scholar 

  • Volm,M., Kaufmann,M., Wayss,K., Goerttler,K., Mattern,J.: Gezielte Tumor-Chemotherapie durch Onkobiogramme. Dtsch. med. Wschr. 99, 38–43 (1974)

    Google Scholar 

  • Wright,J.C., Cobb,J.P., Gumport,S.L., Colomb,F.M., Safadi,S.: Investigation of the relation between clinical and tissue-culture. Response to chemotherapeutic agents on human cancer. New Engl. J. Med. 257, 1207 (1957)

    Google Scholar 

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Authors and Affiliations

  1. Deutsches Krebsforschungszentrum, Institut für experimentelle Pathologie, Heidelberg

    J. Mattern, M. Kaufmann, H. Hinderer, K. Wayss & M. Volm

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  1. J. Mattern
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  2. M. Kaufmann
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  3. H. Hinderer
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  4. K. Wayss
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  5. M. Volm
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Additional information

We are grateful to the Chirurgisches Kinikum and Frauenklinik der Universität Heidelberg and the Krankenhaus Rohrbach (thoraxchirurgische Spezialklinik GmbH) for the supply of human tumor material.

Frauenklinik der Universität Heidelberg.

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Mattern, J., Kaufmann, M., Hinderer, H. et al. Sensitivity tests of tumors to cytostatic agents. Z. Krebsforsch. 83, 97–104 (1975). https://doi.org/10.1007/BF00282976

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  • DOI: https://doi.org/10.1007/BF00282976

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