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Two α-tubulin genes of Aspergillus nidulans encode divergent proteins

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Summary

We have isolated and analyzed the tubA and tubB α-tubulin genes of Aspergillus nidulans. The nucleotide sequences of these genes predict polypeptides of 447 amino acids for tubA and 450 for tubB. The predicted amino acid sequences exhibit 28% divergence between the two polypeptides. This is the second known case of such high divergence between α-tubulins within the same species. The tubB gene is unique in that it codes for an extra glycine residue between what are usually the second and third amino acids. RNA blot analysis demonstrates that the tubA and tubB transcripts are each 1.8 kb long. The level of tubA transcript remains the same throughout the cell cycle. The level of tubB transcript does not change at any particular stage in the cell cycle but increases continuously during spore germination. The tubA gene was previously mapped to linkage group eight, and we have now mapped the tubB gene to linkage group four. Gene disruption in heterokaryons suggests that the phenotypic consequences of disruption are different for the tubA and tubB genes. Molecular disruption of tubA results in a block in nuclear division whereas in tubB it gives rise to abnormal cell and nuclear morphology.

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References

  • Adachi YT, Toda T, Niwa O, Yanagida M (1986) Differential expression of essential and nonessential α-tubulin genes in Schizosaccharomyces pombe. Mol Cell Biol 6:2168–2178

    Google Scholar 

  • Behnke O, Forer A (1967) Evidence for four classes of microtubules in individual cells. J Cell Sci 2:169–192

    Google Scholar 

  • Benton WD, Davis RW (1977) Screening lambda gt recombinant clones by hybridization to single plaque in situ. Science 196:180–182

    Google Scholar 

  • Blattner FR, Williams B, Blech A, Dannison-Thompson K, Faber H, Furlong L, Grunwald D, Keifer D, Moore D, Schumann J, Sheldon E, Smithies O (1977) Charon phages: Safer derivative of bacteriophage lambda for DNA cloning. Science 196:161–169

    Google Scholar 

  • Bond JF, Fridovich-Keil JK, Pillus L, Mulligan RC, Solomon F (1986) A chicken-yeast chimeric β-tubulin protein is incorporated into mouse microtubules in vivo. Cell 4:461–468

    Google Scholar 

  • Brinkley BR, Cartwright J Jr (1970) Organization of microtubules in the mitotic spindle: differential effects of cold shock on microtubule stability. J Cell Biol 57: 25a

  • Brody H, Carbon J (1989) Electrophoretic karyotype of Aspergillus nidulans. Proc Natl Acad Sci USA 86:6260–6263

    Google Scholar 

  • Clements JM, Roberts CF (1986) Transcription and processing signals in the 3′ end of phosphoglycerate kinase gene from Aspergillus nidulans. Gene 44:97–105

    Google Scholar 

  • Cleveland DW, Sullivan KF (1985) Molecular biology and genetics of tubulin. Annu Rev Biochem 54:331–365

    Google Scholar 

  • Cleveland DW, Lopata MA, McDonald RJ, Cowan NJ, Rutter WJ, Kirschner MW (1980) Number and evolutionary conservation of α- and β-tubulins and cytoplasmic γ-actin genes using specific cloned cDNA probes. Cell 20:95–105

    Google Scholar 

  • Clutterbuck AJ (1974) Aspergillus nidulans. In: King RC (ed) Handbook of genetics, vol 1. Plenum, New York, pp 447–510

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  • Gambino J, Bergen LG, Morris NR (1984) Effects of mitotic and tubulin mutations on microtubule architecture in actively growing protoplasts of Aspergillus nidulans. J Cell Biol 99:830–838

    Google Scholar 

  • Gay DA, Yen TJ, Lau JTY, Cleveland DW (1987) Sequences that confer β-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a β-tubulin mRNA. Cell 50:671–679

    Google Scholar 

  • Goode D, Salmon E, Maugel TK, Bonar DB (1975) Microtubule disassembly and recovery from hydrostatic pressure treatment of mitotic HeLa cells. J Cell Biol 67: 138a

  • Huysmans E, Daws E, Van den Berghe A, DeWachter R (1983) The nucleotide sequences of the 5s rRNAs of four mushrooms and their use in studying the phylogenetic position of basidiomycetes among eukaryotes. Nucleic Acids Res 11:2871–2880

    Google Scholar 

  • Joshi HC, Yen TJ, Cleveland DW (1987) In vivo coassembly of a divergent β-tubulin subunit (cβ6) into microtubules of different function. J Cell Biol 105:2179–2190

    Google Scholar 

  • Käfer E (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19:33–131

    Google Scholar 

  • Kaufer NF, Simanis V, Nurse P (1985) Fission yeast Schizosaccharomyces pombe correctly excises a mammalian RNA transcript intervening sequence. Nature 318:78–80

    Google Scholar 

  • Kemphues KJ, Kaufman T, Raff RA, Raff EC (1982) The testis specific β-tubulin subunit in Drosophila melanogaster has multiple functions in spermatogenesis. Cell 31:655–670

    Google Scholar 

  • Kennedy JR, Zimmermann AM (1970) The effects of hydrostatic pressure on microtubles of Tetrahymena pyriformis. J Cell Biol 47:568–576

    Google Scholar 

  • Lewis SA, Gu W, Cowan NJ (1987) Free intermingling of mammalian β-tubulin isotypes among functionally distinct microtubules. Cell 49:539–548

    Google Scholar 

  • Lopata MA, Cleveland DW (1987) In vivo microtubules are copolymers of available β-tubulin isotypes: localization of each of six vertebrate β-tubulin isotypes using polyclonal antibodies elicited by synthetic peptide antigens. J Cell Biol 105:1707–1720

    Google Scholar 

  • Maniatis T, Fritch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • May GS (1989) The highly divergent β-tubulins of Aspergillus nidulans are functionally interchangeable. J Cell Biol 109: 2267–2274

    Google Scholar 

  • May GS, Gambino J, Weatherbee JA, Morris NR (1985) Identification and functional analysis of β-tubulin genes by site-specific integrative transformation in Aspergillus nidulans. J Cell Biol 101:712–719

    Google Scholar 

  • May GS, Tsang ML-S, Smith H, Fidel S, Morris NR (1987) Aspergillus nidulans β-tubulin genes are unusually divergent. Gene 55:231–243

    Google Scholar 

  • May GS, Waring RW, Osmani SA, Morris NR, Denison SH (1989) The coming age of molecular biology in Aspergillus nidulans. Proc EMBO Alko workshop on molecular biology of filamentous fungi, Helsinski 1989. H Nevalainenh, Penttila M (eds) Foundation for Biotechnical and Industrial Fermentation Research 6:11–20

  • Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78

    Google Scholar 

  • Messing J, Crea R, Seaburg H (1981) A system for shotgun DNA sequencing. Nucleic Acids Res 9:309–321

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Moore KC (1972) Pressure induced regression of oral apparatus microtubules in synchronized Tetrahymena. J Ultrastr Res 41:499–518

    Google Scholar 

  • Morris NR, Lai MH, Oakley EC (1979) Identification of a gene for α-tubulin in Aspergillus nidulans. Cell 16:437–442

    Google Scholar 

  • Mount DW, Conrad B (1984) Microcomputer program for graphic analysis of nucleic acid, protein sequences. Nucleic Acids Res 12:811–817

    Google Scholar 

  • Oakley BR, Morris NR (1981b) A β-tubulin mutation in Aspergillus nidulans blocks microtubule function without blocking assembly. Cell 24:837–845

    Google Scholar 

  • Oakley EC, Morris NR (1981b) Phenotype and mapping of tubA1. Aspergillus Newletter 15:39

    Google Scholar 

  • Oakley EC, Oakley BR (1989) Identification of γ-tubulin, a new number of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans. Nature 338:662–664

    Google Scholar 

  • O'Connor TM, Houston LL, Samson F (1974) Stability of neuronal microtubules to high pressure in vivo and in vitro. Proc Natl Acad Sci USA 71:4198–4202

    Google Scholar 

  • Orr WC, Timberlake WE (1982) Clustering of spore specific genes in Aspergillus nidulans. Proc Natl Acad Sci USA 79:5976–5980

    Google Scholar 

  • Osmani SA, May GS, Morris NR (1987) Regulation of the mRNA levels of nimA, a gene required for the G2-M transition in Aspergillus nidulans. J Cell Biol 104:1495–1504

    Google Scholar 

  • Osmani SA, Engle DB, Doonan JH, Morris NR (1988) Spindle formation and chromatin condensation in cells blocked at interphase by mutation of a negative cell cycle control gene. Cell 52:241–251

    Google Scholar 

  • Overbeek PA, Merlino GT, Peters NK, Cohn VH, Moore GP, Kleinsmith LJ (1981) Characterization of five members of the actin gene family in the sea urchin Biochim. Biophys. Acta 656:195–205

    Google Scholar 

  • Ponstingl H, Kraus E, Little M, Kempf T (1981) Complete amino acid sequence of α-tubulin from porcine brain. Proc Natl Acad Sci USA 78:2757–2761

    Google Scholar 

  • Rave N, Crkvenjokov R, Boedtker H (1979) Identification of pro-collagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde gels. Nucleic Acids Res 6:3559–3567

    Google Scholar 

  • Sanger F, Nicklan S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  • Schatz PJ, Pillus L, Grisafi P, Solomon F, Botstein D (1986a) Two functional α-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol Cell Biol 6:3711–3721

    Google Scholar 

  • Schatz PJ, Solomon F, Botstein D (1986b) Genetically essential and non-essential α-tubulin genes specify functionally interchangeable proteins. Mol Cell Biol 6:3722–3733

    Google Scholar 

  • Theurkauf WE, Baum H, Bo J, Wensink PC (1986) Tissue specific and constitutive α-tubulin genes of Drosophila melanogaster code for structurally different proteins. Proc Natl Acad Sci USA 83:8477–8481

    Google Scholar 

  • Toda T, Adachi Y, Hiraoka Y, Yanagida M (1984) Identification of the pleiotropic cell division cycle gene NDA2 as one of the two different α-tubulin genes in S. pombe. Cell 37:233–242

    Google Scholar 

  • Valenzuela P, Quiroga M, Zaldivar J, Rutter WJ, Kirschner MW, Cleveland DW (1981) Nucleotide and corresponding amino acid sequence encoded by α- and β-tubulin mRNAs. Nature 289:650–655

    Google Scholar 

  • Waring RW, May GS, Morris NR (1989) Shuttle vectors and inducible expression vectors using alcA in Aspergillus nidulans. Gene 79:119–130

    Google Scholar 

  • Weatherbee JA, Morris NR (1984) Aspergillus contains multiple tubulin genes. J Biol Chem 259:15452–15459

    Google Scholar 

  • Wilson L, Bamburg JR, Mizel SB, Grisham LM, Creswell KM (1974) Interaction of drugs with microtubule proteins. Fed Proc 33:158–166

    Google Scholar 

  • Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: Nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103–119

    Google Scholar 

  • Yen TJ, Machalin PS, Cleveland DW (1988) Autoregulated instability of β-tubulin mRNAs by recognition of the nascent amino terminus β-tubulin mRNA. Nature 334:580–585

    Google Scholar 

  • Zhengy Y, Oakley BR (1989) Cloning and sequencing of a γ-tubulin cDNA from Drosophila melanogaster. J Cell Biol 109 (4):1856a

    Google Scholar 

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Author notes

  1. Parul Doshi

    Present address: Department of Biology, University of Rochester, 14627, Rochester, NY, USA

  2. John H. Doonan

    Present address: John Innes Institute, NR4 UH, Colney Lane, Norwich, UK

  3. Gregory S. Mays

    Present address: Department of Cell Biology, Baylor School of Medicine, 1200 Moursund Ave, 77030, Houston, TX, USA

Authors and Affiliations

  1. Department of Pharmacology, UMDNJ/Robert Wood Johnson Medical School, 08854, Piscataway, NJ, USA

    Parul Doshi, Cynthia A. Bossie, John H. Doonan, Gregory S. Mays & N. Ronald Morris

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  1. Parul Doshi
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  2. Cynthia A. Bossie
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  3. John H. Doonan
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  4. Gregory S. Mays
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  5. N. Ronald Morris
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Communicated by C. van den Hondel

formerly Rutgers Medical School

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Doshi, P., Bossie, C.A., Doonan, J.H. et al. Two α-tubulin genes of Aspergillus nidulans encode divergent proteins. Molec. Gen. Genet. 225, 129–141 (1991). https://doi.org/10.1007/BF00282651

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  • DOI: https://doi.org/10.1007/BF00282651

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