Summary
The nucleotide sequence has been determined of a 1400 by fragment from the chromosome of Yersinia enterocolitica containing the gene for β-lactamase I. An ORF of 882 by was identified, which could code for a polypeptide of 294 amino acids, closely related to other β-lactamases of molecular class A. Amino acids 1–30 could constitute a signal peptide. The mature protein would be 264 amino acids long with a calculated pI of 6.2. Alignment of the amino acid sequence of the class A β-lactamases suggested the existence of two subgroups in the same class, and this is discussed in the context of the evolution of the enzymes.
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Communicated by W. Goebel
This sequence will appear in the EMBL Data Library under the accession number X57074
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Seoane, A., García Lobo, J.M. Nucleotide sequence of a new class A β-lactamase gene from the chromosome of Yersinia enterocolitica: Implications for the evolution of class A β-lactamases. Molec. Gen. Genet. 228, 215–220 (1991). https://doi.org/10.1007/BF00282468
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DOI: https://doi.org/10.1007/BF00282468