Summary
Chinese hamster x American mink somatic cell hybrids were obtained and examined for chromosome content and expression of mink malate dehydrogenase, NADP (MOD-1; EC 1.1.1.40), malate dehydrogenase, NAD (MOR-1; EC 1.1.1.37), glucose-6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) and hypoxanthine phosphoribosyltransferase (HPRT; EC 2.4.2.8). All the hybrid clones examined were found to segregate mink chromosomes. A clone panel containing 25 clones was set up. The possibilities and limitations of this panel for mink gene mapping are analysed. Using this panel, it is feasible to rapidly map genes located on chromosomes 1–13 and to provisionally assign genes located on chromosome 14 and the X. Based on the data obtained, the genes for MOD-1 and MOR-1 were firmly assigned to mink chromosomes 1 and 11, respectively, and the genes for G6PD and HPRT were provisionally assigned to the X.
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Literature
Bakay, B.; Graf, M.; Carey, S.; Wissinen, E.; Nyhan, W.L. (1978): Reexpression of HPRT activity following cell fusion with polyethylene glycol. Biochem. Genet. 16, 227–237
Baker, W.W.; Mintz, B. (1969): Subunit structure and gene control of mouse NADP-malate dehydrogenase. Biochem. Genet. 2, 351–360
Cowmeadow, M.P.; Ruddle, F.H. (1978): Computer-assisted statistical procedures for somatic gene assignment. Cytogenet. Cell Genet. 22, 694–697
Giles, R.E.; Ruddle, F.H. (1973): Production and characterization of proliferating somatic cell hybrids. In: Tissue Culture: Methods and Applications (eds.: Kruse, P.F.; Patterson, M.K.), pp. 475–500. New York: Acad. Press
Hu, F.; Pastor, L.M.; Teramura, D.J. (1977): Somatic cell hybrids derived from terminally differentiated rhesus cells and established mouse cell lines. Mech. Ageing Develop. 6, 305–318
Littlefield, J.W. (1964): Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145, 709–710
Mandahl, N.; Fredga, K. (1975): Q, G and C-band patterns of the mink chromosomes. Hereditas 81, 211–220
Nichols, E.A.; Ruddle, F.H. (1979): Comparative sensitivies of electrophoretic assays for human enzymes. Biochem. Genet. 17, 123–132
Pearson, P.L.; Roderick, T.H.; Davisson, M.T.; Garver, J.J.; Warburton, D.; Lalley, P.A.; O'Brien, S.J. (1979): Report of the committee on comparative mapping. Cytogenet. Cell Genet. 25, 82–95
Petrova, O.N.; Khalisev, A.E.; Grigorjeva, G.A.; Shapiro, N.I. (1967): Studies of hereditary resistance to the analogues of purine bases in mammalian cells cultured in vitro. Genetika (Russ.) 3, 74–92
Porter, I.H.; Boyer, S.H.; Watson-Williams, E.J.; Adam, A.; Steinberg, A.; Siniscalco, M. (1964): Variation of glucose-6-phosphate dehydrogenase in different human populations. Lancet 1, 895–899
Radjabli, S.I.; Krukova, H.P. (1973): A comparison of banding patterns in two hamster species: Cricetulus barabensis and C. griseus. Tsitologia (Rss.) 15, 1527–1538
Reznik, L.G.; Reshetnikova, G.F.; Radjabli, S.I. Establishment and chromosome analysis of a new cell line of American mink (Mustek vison) (in press)
Serov, O.L.; Zakijan, S.M.; Khlebodarova, T.M.; Korochkin, L.I. (1976): Allelic expression in intergeneric fox hybrids (Alopex lagopus x Vulpes vulpes). I. Comparative electrophoretic study on blood enzymes and proteins in Arctic and silver foxes. Biochem. Genet. 14, 1091–1103
Serov, O.L.; Zakijan, S.M.; Kulichkov, V.A. (1978): Allelic expression in intergeneric fox hybrids (Alopex lagopus x Vulpes vulpes). III. Regulation of the expression of the parental alleles at the Gpd locus linked to the X chromosome. Biochem. Genet. 16, 145–157
Serov, O.L.; Belyaev, N.D.; Vakhrusheva, T.M.; Zakijan, S.M.; Pressman, E.K. (1979): Intracellular mechanisms of the formation of homo- and heteropolymeric isozymes. I. Kinetics of the formation of the heteropolymeric isozyme of malate dehydrogenase in parasexual hybrids of two Acetabularia species. Biochem. Genet. 17, 965–986
Shows, T.B.; Ruddle, F.H. (1968): Malate dehydrogenase: evidence for tetrameric structure in Mus musculus. Science 160, 1356–1357
Weiss, M.; Ephrussi, B. (1966): Studies of interspecific (rat x mouse) somatic hybrids. I. isolation, growth and evolution of the karyotype. Genetics 54, 1095–1109
Wijnen, L.M.M.; Grzeschik, K.H.; Pearson, P.L.; Meera Khan, P. (1977): The human PGM-2 and its chromosomal localization in man-mouse hybrids. Hum. Genet. 37, 271–278
Yerganian, G.; Leonard, M.J. (1961): Maintenance of normal in situ chromosomal features in long-term tissue cultures. Science 133, 1600–1601
Zakharov, A.F.; Kakpakova, E.S.; Egolina, N.A.; Pogosianz, H.E. (1964): Chromosomal variability in clonal populations of a Chinese hamster cell strain. J. Nat. Cancer Inst. 33, 935–956
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Rubtsov, N.B., Radjabli, S.I., Gradov, A.A. et al. Chinese hamster x American mink somatic cell hybrids: characterization of a clone panel and assignment of the mink genes for malate dehydrogenase, NADP-1 and malate dehydrogenase, NAD-1. Theoret. Appl. Genetics 60, 99–106 (1981). https://doi.org/10.1007/BF00282425
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DOI: https://doi.org/10.1007/BF00282425