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QTL mapping of naturally-occurring variation in flowering time of Arabidopsis thaliana

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Abstract

A segregating F2 population of Arabidopsis thaliana derived from a cross between the late-flowering ecotype Hannover/Münden (HM) and the early-flowering ecotype Wassilewskija (WS) was analyzed for flowering time and other morphological traits. Two unlinked quantitative trait loci (QTLs) affecting days to first flower (DFF-a and DFF-b) mapped to chromosome 5. QTLs which affect node number (NN), leaf length at flowering (LLF), and leaf length at 35 days (LL35) also mapped to chromosome 5; LLF-a, LL35-a, NN-a map to the same region of chromosome 5 as DFF-a; LLF-b and LL35-bmap to the same region of chromosome 5 as DFF-b. Another QTL affecting leaf length at flowering (LLF-c) maps to chromosome 3. The proximity of DFF-a, LLF-a, LL35-a and NN-a, as well as the similarity in gene action among these QTLs (additivity), suggest that they may be pleiotropic consequences of a single gene at this locus. Similarly, LL35-b and LLF-b map near each other and both display recessive gene action, again suggesting the possibility of pleiotropy. DFF-b, which also maps near LL35-b and LLF-b, displays largely additive gene action (although recessive gene action could not be ruled out). This suggests that DFF-b may represent a different gene from LL35-b and/or LLF-b. DFF-a maps near two previously identified mutants: co (which also affects flowering time and displays gene action consistent with additivity) and flc. Similar map locations and gene actions of QTLs affecting the correlated traits DFF, LLF, LL35 and NN suggest that these genomic regions harbor naturally occurring allelic variants involved in the general transition of the plant from vegetative to reproductive growth.

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Authors and Affiliations

  1. Department of Soil and Crop Sciences, Texas A&M University, 77843-2474, College Station, TX, USA

    Stanley P. Kowalski, Tien-Hung Lan & Andrew H. Paterson

  2. Department of Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA

    Kenneth A. Feldmann

  3. Department of Plant and Soil Sciences, University of Delaware, 19711, Newark, DE, USA

    Andrew H. Paterson

Authors
  1. Stanley P. Kowalski
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  2. Tien-Hung Lan
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  3. Kenneth A. Feldmann
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  4. Andrew H. Paterson
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Communicated by H. Saedler

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Kowalski, S.P., Lan, TH., Feldmann, K.A. et al. QTL mapping of naturally-occurring variation in flowering time of Arabidopsis thaliana . Molec. Gen. Genet. 245, 548–555 (1994). https://doi.org/10.1007/BF00282217

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